β-Estradiol and progesterone enhance biofilm development and persister cell formation in monospecies and microcosms biofilms derived from vulvovaginal candidiasis.

IF 2 3区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biofouling Pub Date : 2023-08-01 Epub Date: 2023-09-12 DOI:10.1080/08927014.2023.2256674

Ana Raquel Colares de Andrade, Mônica Dantas Sampaio Rezende, Fernando Victor Monteiro Portela, Livia Maria Galdino Pereira, Bruno Nascimento da Silva, Reginaldo Gonçalves de Lima-Neto, Marcos Fábio Gadelha Rocha, José Júlio Costa Sidrim, Débora Souza Collares Maia Castelo-Branco, Rossana de Aguiar Cordeiro

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Abstract

The present study aimed to: (1) evaluate the influence of the steroid hormones (SH) on biofilm development; (2) investigate the formation of persister cells (PC) in biofilms; and (3) investigate the influence of SH on PC formation. Biofilms were derived from vulvovaginal candidiasis (VVC) samples and evaluated by three models: microcosm biofilms grown in Vaginal Fluid Simulator Medium (MiB-VFSM); monospecies biofilms grown in VFSM (MoB-VFSM) and RPMI media (MoB-RPMI). SH altered cell counting and biomass of biofilms grown in VSFM; MoB-RPMI were negatively affected by SH. SH stimulated the formation of PC in MiB-VFSM but not MoB-VFSM; MoB-RPMI showed a lower number of PC in the presence of SH. The results showed that SH altered the dynamics of biofilm formation and development, depending on the study model. The data suggest the influence of hormones on the physiology of Candida biofilms and reinforce the importance of PC in the pathogenesis of VVC.

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β-雌二醇和黄体酮可促进外阴阴道念珠菌病单种和微种生物膜中生物膜的发育和持久细胞的形成。

本研究旨在：（1）评价类固醇激素（SH）对生物膜发育的影响；（2）研究生物膜中持久细胞（PC）的形成；（3）研究SH对PC形成的影响。生物膜来源于外阴阴道念珠菌病（VVC）样本，并通过三个模型进行评估：在阴道液模拟培养基（MiB-VFSM）中生长的微宇宙生物膜；在VFSM（MoB-VFSM）和RPMI培养基（MoB-RPMI）中生长的单物种生物膜。SH改变了VSFM中生长的生物膜的细胞计数和生物量；SH可刺激MiB-VFSM中PC的形成，但不刺激MoB-VFSM；在SH存在的情况下，MoB-RPMI显示出较低的PC数量。结果表明，SH改变了生物膜形成和发育的动力学，这取决于研究模型。这些数据表明激素对念珠菌生物膜生理的影响，并加强了PC在VVC发病机制中的重要性。

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来源期刊

Biofouling 生物-海洋与淡水生物学

CiteScore

5.00

自引率

7.40%

发文量

审稿时长

1.7 months

期刊介绍： Biofouling is an international, peer-reviewed, multi-discliplinary journal which publishes original articles and mini-reviews and provides a forum for publication of pure and applied work on protein, microbial, fungal, plant and animal fouling and its control, as well as studies of all kinds on biofilms and bioadhesion. Papers may be based on studies relating to characterisation, attachment, growth and control on any natural (living) or man-made surface in the freshwater, marine or aerial environments, including fouling, biofilms and bioadhesion in the medical, dental, and industrial context. Specific areas of interest include antifouling technologies and coatings including transmission of invasive species, antimicrobial agents, biological interfaces, biomaterials, microbiologically influenced corrosion, membrane biofouling, food industry biofilms, biofilm based diseases and indwelling biomedical devices as substrata for fouling and biofilm growth, including papers based on clinically-relevant work using models that mimic the realistic environment in which they are intended to be used.