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Effect of promethazine against Staphylococcus aureus and its preventive action in the formation of biofilms on silicone catheters. 异丙嗪对金黄色葡萄球菌的影响及其在硅胶导管上形成生物膜的预防作用。
IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-04-04 DOI: 10.1080/08927014.2025.2486250
Érica Rayanne Mota da Costa, Vitória Pessoa de Farias Cabral, Daniel Sampaio Rodrigues, Thais Lima Ferreira, Maria Janielly Castelo Branco Silveira, Leilson Carvalho de Oliveira, Lívia Gurgel do Amaral Valente Sá, Cecília Rocha da Silva, João Batista de Andrade Neto, Sorele Facundo da Silva, Bruno Coêlho Cavalcanti, Manoel Odorico de Moraes, Hélio Vitoriano Nobre Júnior

Urinary infections caused by Staphylococcus aureus are commonly associated with urinary catheterization and often result in severe complications. Given this problem, the objective of the study was to investigate the preventive action of promethazine (PMT) against the formation of methicillin-resistant Staphylococcus aureus (MRSA) biofilms when impregnated in urinary catheters. For this purpose, techniques such as broth microdilution, checkerboard, impregnation on urinary catheter fragments, flow cytometry assays and scanning electron microscopy were employed. PMT exhibited antimicrobial activity with Minimum Inhibitory Concentration (MIC) values ranging from 171 to 256 µg/mL, predominantly additive interaction in combination with oxacillin (OXA) and vancomycin (VAN), and a reduction in cell viability of biofilms formed and forming by methicillin-sensitive and -resistant S. aureus. Morphological alterations, damage to the membrane, and genetic material of cells treated with promethazine were also observed. The results demonstrated that PMT can be classified as a promising antimicrobial agent for use in the antibacterial coating of long-term urinary devices.

{"title":"Effect of promethazine against <i>Staphylococcus aureus</i> and its preventive action in the formation of biofilms on silicone catheters.","authors":"Érica Rayanne Mota da Costa, Vitória Pessoa de Farias Cabral, Daniel Sampaio Rodrigues, Thais Lima Ferreira, Maria Janielly Castelo Branco Silveira, Leilson Carvalho de Oliveira, Lívia Gurgel do Amaral Valente Sá, Cecília Rocha da Silva, João Batista de Andrade Neto, Sorele Facundo da Silva, Bruno Coêlho Cavalcanti, Manoel Odorico de Moraes, Hélio Vitoriano Nobre Júnior","doi":"10.1080/08927014.2025.2486250","DOIUrl":"https://doi.org/10.1080/08927014.2025.2486250","url":null,"abstract":"<p><p>Urinary infections caused by <i>Staphylococcus aureus</i> are commonly associated with urinary catheterization and often result in severe complications. Given this problem, the objective of the study was to investigate the preventive action of promethazine (PMT) against the formation of methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) biofilms when impregnated in urinary catheters. For this purpose, techniques such as broth microdilution, checkerboard, impregnation on urinary catheter fragments, flow cytometry assays and scanning electron microscopy were employed. PMT exhibited antimicrobial activity with Minimum Inhibitory Concentration (MIC) values ranging from 171 to 256 µg/mL, predominantly additive interaction in combination with oxacillin (OXA) and vancomycin (VAN), and a reduction in cell viability of biofilms formed and forming by methicillin-sensitive and -resistant <i>S. aureus</i>. Morphological alterations, damage to the membrane, and genetic material of cells treated with promethazine were also observed. The results demonstrated that PMT can be classified as a promising antimicrobial agent for use in the antibacterial coating of long-term urinary devices.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"1-18"},"PeriodicalIF":2.6,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Considerations for testing anti-fouling coatings designed for implementation into Earth-based and spacecraft water systems.
IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-03-26 DOI: 10.1080/08927014.2025.2479692
Madelyn K Mettler, Erika J Espinosa-Ortiz, Darla M Goeres, Brent M Peyton

Biofilms are common in water systems and can lead to mechanical failure or illness of water system users. Methods for evaluating anti-fouling coatings have largely been informed by the medical industry and have not been tailored to industrial or spacecraft water systems. The goal of the paper is to help guide researchers in designing experiments to evaluate coatings that accurately represent the system under investigation. This review identified eight experimental design considerations when evaluating coatings in water systems: biofilm reactor operation, microorganism selection, reinoculation, coating surface area, liquid medium, experiment duration, coating performance evaluation, and the use of microgravity. The impact of each decision made within each of these considerations is presented. Further, the methods featured in eight studies investigating coatings for Earth-based or spacecraft water systems are discussed. This review serves to guide researchers toward improved experimental design to enable successful technology transfer from the lab bench to Earth and beyond.

{"title":"Considerations for testing anti-fouling coatings designed for implementation into Earth-based and spacecraft water systems.","authors":"Madelyn K Mettler, Erika J Espinosa-Ortiz, Darla M Goeres, Brent M Peyton","doi":"10.1080/08927014.2025.2479692","DOIUrl":"https://doi.org/10.1080/08927014.2025.2479692","url":null,"abstract":"<p><p>Biofilms are common in water systems and can lead to mechanical failure or illness of water system users. Methods for evaluating anti-fouling coatings have largely been informed by the medical industry and have not been tailored to industrial or spacecraft water systems. The goal of the paper is to help guide researchers in designing experiments to evaluate coatings that accurately represent the system under investigation. This review identified eight experimental design considerations when evaluating coatings in water systems: biofilm reactor operation, microorganism selection, reinoculation, coating surface area, liquid medium, experiment duration, coating performance evaluation, and the use of microgravity. The impact of each decision made within each of these considerations is presented. Further, the methods featured in eight studies investigating coatings for Earth-based or spacecraft water systems are discussed. This review serves to guide researchers toward improved experimental design to enable successful technology transfer from the lab bench to Earth and beyond.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"1-19"},"PeriodicalIF":2.6,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of adhesion and fouling-release properties of non-toxic fouling-release coatings applied to oil boom materials.
IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-03-26 DOI: 10.1080/08927014.2025.2480175
Jackson Benda, Madhura Pade, Ihor Tarnavchyk, Ryan Burgett, Jonas Sahouani, James Bahr, Kailey Richard, Kelli Z Hunsucker, Geoffrey Swain, Shane Stafslien, Dean C Webster

Oil booms are used in the containment of oil leaking from ships moored in harbors around the world. However, marine biofouling quickly accumulates on these materials. The application of coatings offers a potential solution by preventing the growth of these organisms, but issues with adhesion of coatings to the oil boom materials remain a large barrier to success. This work is focused on testing the adhesion of marine coatings systems to oil boom fabrics after use of surface treatments or adhesion promoters. Surface analysis of fabrics showed a large variation in surface energy, contributing to the adhesion of coatings to these substrates. A laboratory water-jet adhesion-screening test identified several combinations of surface treatments and adhesion promoters that improved adhesion of coatings to various oil boom substrates. Large-scale field-testing of these candidates displayed improved cleanability, which translated into an increase in service life for coated oil boom fabrics.

{"title":"Evaluation of adhesion and fouling-release properties of non-toxic fouling-release coatings applied to oil boom materials.","authors":"Jackson Benda, Madhura Pade, Ihor Tarnavchyk, Ryan Burgett, Jonas Sahouani, James Bahr, Kailey Richard, Kelli Z Hunsucker, Geoffrey Swain, Shane Stafslien, Dean C Webster","doi":"10.1080/08927014.2025.2480175","DOIUrl":"https://doi.org/10.1080/08927014.2025.2480175","url":null,"abstract":"<p><p>Oil booms are used in the containment of oil leaking from ships moored in harbors around the world. However, marine biofouling quickly accumulates on these materials. The application of coatings offers a potential solution by preventing the growth of these organisms, but issues with adhesion of coatings to the oil boom materials remain a large barrier to success. This work is focused on testing the adhesion of marine coatings systems to oil boom fabrics after use of surface treatments or adhesion promoters. Surface analysis of fabrics showed a large variation in surface energy, contributing to the adhesion of coatings to these substrates. A laboratory water-jet adhesion-screening test identified several combinations of surface treatments and adhesion promoters that improved adhesion of coatings to various oil boom substrates. Large-scale field-testing of these candidates displayed improved cleanability, which translated into an increase in service life for coated oil boom fabrics.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"1-15"},"PeriodicalIF":2.6,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An improved method for quantitatively measuring antifouling coating performance using a mussel single thread tensile adhesion test.
IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-03-18 DOI: 10.1080/08927014.2025.2476491
Samuel M Pennell, Thomas B LeFevre, Jonathan Bennett, Wilaiwan Chouyyok, Joseph D Daddona, Raymond S Addleman, Curtis J Larimer, George T Bonheyo

Surface biofouling reduces the efficiency and lifespan of equipment across many industries. The development of high-performance antifouling surfaces, such as foul release coatings, benefits from test methods that can quickly identify superior antifouling surfaces in the laboratory during material development. Existing test methods poorly discriminate between different foul release coatings. Here is presented a method to assess the ability of surfaces to resist mussel adhesion using a quantitative, controlled single thread adhesion test (STAT) method, allowing for meaningful comparisons between low adhesion foul release surfaces. This method provides greater accuracy and finer resolution than push-based mussel shear adhesion methods without the difficulties associated with mussel size, thread attachment angle, or harming the mussels. The single thread tensile method is demonstrated on a variety of standard and high-performance coatings, and it is shown that the method detects differentiation between commercial foul release coatings that could not be resolved using other methods.

{"title":"An improved method for quantitatively measuring antifouling coating performance using a mussel single thread tensile adhesion test.","authors":"Samuel M Pennell, Thomas B LeFevre, Jonathan Bennett, Wilaiwan Chouyyok, Joseph D Daddona, Raymond S Addleman, Curtis J Larimer, George T Bonheyo","doi":"10.1080/08927014.2025.2476491","DOIUrl":"10.1080/08927014.2025.2476491","url":null,"abstract":"<p><p>Surface biofouling reduces the efficiency and lifespan of equipment across many industries. The development of high-performance antifouling surfaces, such as foul release coatings, benefits from test methods that can quickly identify superior antifouling surfaces in the laboratory during material development. Existing test methods poorly discriminate between different foul release coatings. Here is presented a method to assess the ability of surfaces to resist mussel adhesion using a quantitative, controlled single thread adhesion test (STAT) method, allowing for meaningful comparisons between low adhesion foul release surfaces. This method provides greater accuracy and finer resolution than push-based mussel shear adhesion methods without the difficulties associated with mussel size, thread attachment angle, or harming the mussels. The single thread tensile method is demonstrated on a variety of standard and high-performance coatings, and it is shown that the method detects differentiation between commercial foul release coatings that could not be resolved using other methods.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"1-12"},"PeriodicalIF":2.6,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The influence of surface materials on microbial biofilm formation in aviation fuel systems.
IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-03-10 DOI: 10.1080/08927014.2025.2471366
Juan F Mujica-Alarcon, Jaime Gomez-Bolivar, James Barnes, Myrsini Chronopoulou, Jesus J Ojeda, Steven F Thornton, Stephen A Rolfe

The ability of different microbes to form biofilms on materials found in aviation fuel systems was assessed using both individual isolates and complex microbial communities. Biofilm formation by the Gram-negative bacterium, Pseudomonas putida, the fungus Amorphotheca resinae and the yeast, Candida tropicalis, was influenced by material surface properties although this differed between isolates. Biofilm formation was greatest at the fuel-water interface. The Gram-positive bacterium Rhodococcus erythropolis, in contrast, was able to grow on most surfaces. When a subset of materials was exposed to complex microbial communities, the attached microbial community structure was influenced by surface properties and selected for different genera best able to form biofilms on a specific surface. Distinct sub-populations of Pseudomonads were identified, which favoured growth on aluminium or painted surfaces, with a different subpopulation favouring growth on nitrile.

{"title":"The influence of surface materials on microbial biofilm formation in aviation fuel systems.","authors":"Juan F Mujica-Alarcon, Jaime Gomez-Bolivar, James Barnes, Myrsini Chronopoulou, Jesus J Ojeda, Steven F Thornton, Stephen A Rolfe","doi":"10.1080/08927014.2025.2471366","DOIUrl":"https://doi.org/10.1080/08927014.2025.2471366","url":null,"abstract":"<p><p>The ability of different microbes to form biofilms on materials found in aviation fuel systems was assessed using both individual isolates and complex microbial communities. Biofilm formation by the Gram-negative bacterium, <i>Pseudomonas putida</i>, the fungus <i>Amorphotheca resinae</i> and the yeast, <i>Candida tropicalis</i>, was influenced by material surface properties although this differed between isolates. Biofilm formation was greatest at the fuel-water interface. The Gram-positive bacterium <i>Rhodococcus erythropolis</i>, in contrast, was able to grow on most surfaces. When a subset of materials was exposed to complex microbial communities, the attached microbial community structure was influenced by surface properties and selected for different genera best able to form biofilms on a specific surface. Distinct sub-populations of <i>Pseudomonads</i> were identified, which favoured growth on aluminium or painted surfaces, with a different subpopulation favouring growth on nitrile.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"1-18"},"PeriodicalIF":2.6,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A modified formulation of Zoono GermFree24 antiseptic liquid has enhanced efficacy against biofilms of Staphylococcus aureus and Pseudomonas aeruginosa.
IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-03-09 DOI: 10.1080/08927014.2025.2467078
Samuel J M Hale, Christian A Lux, Raymond Kim, Kristi Biswas, Brett Wagner Mackenzie, Richard G Douglas

Zoono GermFree24 is a quaternary ammonium compound-based hand sanitiser. Its efficacy against planktonic bacteria is well established, but efficacy against biofilms has not been tested. We investigated the antibiofilm efficacy of Zoono GermFree24 hand sanitiser and a modified formulation against biofilms of Staphylococcus aureus ATCC 6538 and Pseudomonas aeruginosa ATCC 27853 grown in vitro using the Centers for Disease Control (CDC) biofilm reactor and 96-pin lids. Biofilms were immersed in Zoono GermFree24 or Zoono B22-1402A (modified formulation) for 5 min, 1 h, 6 h or overnight (22 ± 2 h). The antiseptic was neutralised and the bacteria remaining after treatment were cultured and quantified. Zoono GermFree24 and Zoono B22-1402A caused time-dependent reductions in viable biofilms of both species with both methods of culture and testing, with more rapid biofilm eradication observed for Zoono B22-1402A. Biofilms grown on 96-pin lids were more quickly eradicated than those grown in the CDC biofilm reactor.

{"title":"A modified formulation of <i>Zoono GermFree24</i> antiseptic liquid has enhanced efficacy against biofilms of <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i>.","authors":"Samuel J M Hale, Christian A Lux, Raymond Kim, Kristi Biswas, Brett Wagner Mackenzie, Richard G Douglas","doi":"10.1080/08927014.2025.2467078","DOIUrl":"10.1080/08927014.2025.2467078","url":null,"abstract":"<p><p><i>Zoono GermFree24</i> is a quaternary ammonium compound-based hand sanitiser. Its efficacy against planktonic bacteria is well established, but efficacy against biofilms has not been tested. We investigated the antibiofilm efficacy of <i>Zoono GermFree24</i> hand sanitiser and a modified formulation against biofilms of <i>Staphylococcus aureus</i> ATCC 6538 and <i>Pseudomonas aeruginosa</i> ATCC 27853 grown <i>in vitro</i> using the Centers for Disease Control (CDC) biofilm reactor and 96-pin lids. Biofilms were immersed in <i>Zoono GermFree24</i> or <i>Zoono</i> B22-1402A (modified formulation) for 5 min, 1 h, 6 h or overnight (22 ± 2 h). The antiseptic was neutralised and the bacteria remaining after treatment were cultured and quantified. <i>Zoono GermFree24</i> and <i>Zoono</i> B22-1402A caused time-dependent reductions in viable biofilms of both species with both methods of culture and testing, with more rapid biofilm eradication observed for <i>Zoono</i> B22-1402A. Biofilms grown on 96-pin lids were more quickly eradicated than those grown in the CDC biofilm reactor.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"1-9"},"PeriodicalIF":2.6,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of morin and carvacrol loaded-nanoparticles on oral polymicrobial biofilm control.
IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-03-03 DOI: 10.1080/08927014.2025.2471975
Luciana Solera Sales, Amanda Letícia Polli Silvestre, Marlus Chorilli, Andréia Bagliotti Meneguin, Hernane da Silva Barud, Fernanda Lourenção Brighenti

This study aimed to develop and characterize sodium alginate (SA)/chitosan (CS) based nanoparticles (NPs), with or without morin or carvacrol, and to evaluate the antimicrobial and antibiofilm activity against polymicrobial oral biofilms. Three different NPs (0.15:1; 0.3:1; 0.5:1 CS:SA) whether or not containing morin or carvacrol were developed and characterized by particle size, zeta potential, scanning electron microscope (SEM), encapsulation efficiency, and in vitro drug release. NPs antibiofilm and antimicrobial activity were evaluated using polymicrobial oral biofilms by means of quantifying the biomass, assessment of viable microorganisms (CFU/mL), and acidogenicity of the biofilm by pH readings. The NPs presented nanometric size (<500 nm), with spherical shape and smooth surface. Encapsulation efficiency of the samples containing morin ranged from 46.17 to 55.15% and for carvacrol from 55.30 to 90.15%. Total release of carvacrol and morin occurred within 15 min. The NPs significantly reduced biofilm biomass and microbial viability compared to the control. However, did not significantly increase the biofilm pH. The NPs were effectively synthesized and showed antimicrobial and antibiofilm effect against oral biofilm and the addition of natural substances morin or carvacrol increased this effect. Combination of chitosan and sodium alginate and addition of morin or carvacrol in NPs can be a promising strategy for oral use, fighting biofilm and consequently biofilm dependent diseases.

{"title":"Evaluation of morin and carvacrol loaded-nanoparticles on oral polymicrobial biofilm control.","authors":"Luciana Solera Sales, Amanda Letícia Polli Silvestre, Marlus Chorilli, Andréia Bagliotti Meneguin, Hernane da Silva Barud, Fernanda Lourenção Brighenti","doi":"10.1080/08927014.2025.2471975","DOIUrl":"https://doi.org/10.1080/08927014.2025.2471975","url":null,"abstract":"<p><p>This study aimed to develop and characterize sodium alginate (SA)/chitosan (CS) based nanoparticles (NPs), with or without morin or carvacrol, and to evaluate the antimicrobial and antibiofilm activity against polymicrobial oral biofilms. Three different NPs (0.15:1; 0.3:1; 0.5:1 CS:SA) whether or not containing morin or carvacrol were developed and characterized by particle size, zeta potential, scanning electron microscope (SEM), encapsulation efficiency, and <i>in vitro</i> drug release. NPs antibiofilm and antimicrobial activity were evaluated using polymicrobial oral biofilms by means of quantifying the biomass, assessment of viable microorganisms (CFU/mL), and acidogenicity of the biofilm by pH readings. The NPs presented nanometric size (<500 nm), with spherical shape and smooth surface. Encapsulation efficiency of the samples containing morin ranged from 46.17 to 55.15% and for carvacrol from 55.30 to 90.15%. Total release of carvacrol and morin occurred within 15 min. The NPs significantly reduced biofilm biomass and microbial viability compared to the control. However, did not significantly increase the biofilm pH. The NPs were effectively synthesized and showed antimicrobial and antibiofilm effect against oral biofilm and the addition of natural substances morin or carvacrol increased this effect. Combination of chitosan and sodium alginate and addition of morin or carvacrol in NPs can be a promising strategy for oral use, fighting biofilm and consequently biofilm dependent diseases.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"1-17"},"PeriodicalIF":2.6,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Carbon dioxide infusion reduces invasive mussel biofouling (quagga mussel; Dreissena rostriformis bugensis) in raw water systems.
IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-02-25 DOI: 10.1080/08927014.2025.2468282
Matthew T Barbour, Todd J Severson, Jeremy K Wise, Matthew J Muelemans, Kevin Kelly, Sherri Pucherelli, Diane L Waller

The efficacy of carbon dioxide (CO2) to reduce biofouling by quagga mussels (Dreissena rostriformis bugensis) in raw water systems was investigated. Experiments were conducted in a mobile laboratory located at Bureau of Reclamation Davis Dam Hydropower Facility and supplied with raw water from Lake Mohave, a reservoir of the Colorado River, USA. Incoming water was split between five chambers, each infused with CO2 at a different rate. Raw reservoir water containing quagga larvae (veligers) was mixed with CO2 chamber outflows and delivered to tanks containing settlement plates. Two experiments were conducted. Experiment 1 tested continuous infusion at target concentrations of 30, 45, 60, 75, and 100 mg L-1 dCO2 (dissolved CO2). Experiment 2 evaluated intermittent infusion schedules: 24 h on/off with 50, 75, and 100 mg L-1 dCO2 and 24 h once/week with 100 mg L-1 dCO2. In Experiment 1, the percent settlement decreased with mean CO2 concentration, ranging from 5.0% to < 0.1% in 28.7 and 92.2 mg L-1 dCO2, respectively. In Experiment 2, the efficacy of 24 h on/off at dCO2 > 72.2 mg L-1 was similar to continuous treatment. The least effective treatment was 24 h once weekly at 95 mg L-1 dCO2. These results demonstrate that CO2 treatment may reduce mussel biofouling in raw water systems.

{"title":"Carbon dioxide infusion reduces invasive mussel biofouling (quagga mussel; <i>Dreissena rostriformis bugensis</i>) in raw water systems.","authors":"Matthew T Barbour, Todd J Severson, Jeremy K Wise, Matthew J Muelemans, Kevin Kelly, Sherri Pucherelli, Diane L Waller","doi":"10.1080/08927014.2025.2468282","DOIUrl":"https://doi.org/10.1080/08927014.2025.2468282","url":null,"abstract":"<p><p>The efficacy of carbon dioxide (CO<sub>2</sub>) to reduce biofouling by quagga mussels (<i>Dreissena rostriformis bugensis</i>) in raw water systems was investigated. Experiments were conducted in a mobile laboratory located at Bureau of Reclamation Davis Dam Hydropower Facility and supplied with raw water from Lake Mohave, a reservoir of the Colorado River, USA. Incoming water was split between five chambers, each infused with CO<sub>2</sub> at a different rate. Raw reservoir water containing quagga larvae (veligers) was mixed with CO<sub>2</sub> chamber outflows and delivered to tanks containing settlement plates. Two experiments were conducted. Experiment 1 tested continuous infusion at target concentrations of 30, 45, 60, 75, and 100 mg L<sup>-1</sup> dCO<sub>2</sub> (dissolved CO<sub>2</sub>). Experiment 2 evaluated intermittent infusion schedules: 24 h on/off with 50, 75, and 100 mg L<sup>-1</sup> dCO<sub>2</sub> and 24 h once/week with 100 mg L<sup>-1</sup> dCO<sub>2</sub>. In Experiment 1, the percent settlement decreased with mean CO<sub>2</sub> concentration, ranging from 5.0% to < 0.1% in 28.7 and 92.2 mg L<sup>-1</sup> dCO<sub>2</sub>, respectively. In Experiment 2, the efficacy of 24 h on/off at dCO<sub>2</sub> > 72.2 mg L<sup>-1</sup> was similar to continuous treatment. The least effective treatment was 24 h once weekly at 95 mg L<sup>-1</sup> dCO<sub>2</sub>. These results demonstrate that CO<sub>2</sub> treatment may reduce mussel biofouling in raw water systems.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"1-12"},"PeriodicalIF":2.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of epigallocatechin gallate on the development of matrix-rich Streptococcus mutans biofilm. 表没食子儿茶素没食子酸酯对富基质变形链球菌生物膜发育的影响。
IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-02-01 Epub Date: 2025-01-02 DOI: 10.1080/08927014.2024.2446932
Maria Gerusa Brito Aragão, Carolina Patricia Aires, Silmara Aparecida Milori Corona, Xuesong He

In this study, we evaluated the impact of Epigalocatechin-3-gallate (EGCG) on S. mutans biofilm development for 24 and 46 h using high-resolution confocal laser scanning microscopy. EGCG treatment led to the formation of interspaced exopolysaccharide (EPS)-microcolony complexes unevenly distributed on the surface of hydroxyapatite disc, forming a thinner and less complex biofilm structure with significantly reduced biomass, matrix volume, and thickness compared to the NaCl treated group (negative control). At 46 h, the biofilm of the EGCG-treatment group failed to form the bacterial-EPS superstructures which is characteristic of the biofilm in the negative control group. EGCG treatment seems to significantly delay biofilm development, with the 46 h biofilm in the EGCG treatment group resembling the negative control group at 24 h. EGCG topical treatments impaired S. mutans biofilm initial growth and maturation, suggesting its potential to be used as a preventive agent against dental caries.

在这项研究中,我们使用高分辨率共聚焦激光扫描显微镜评估了表没食子儿茶素-3-没食子酸酯(EGCG)对变形链球菌生物膜发育24和46 h的影响。EGCG处理导致间隙外多糖(EPS)-微菌落复合物不均匀分布在羟基磷灰石盘表面,形成更薄、更不复杂的生物膜结构,生物量、基质体积和厚度均显著低于NaCl处理组(阴性对照)。46 h时,egcg处理组生物膜未形成阴性对照组生物膜特有的细菌- eps超结构。EGCG处理似乎显著延缓了生物膜的发育,EGCG处理组46 h的生物膜与阴性对照组24 h的相似。EGCG外用治疗会损害变形链球菌生物膜的初始生长和成熟,提示其可能被用作预防龋齿的药物。
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引用次数: 0
Anti-biofilm effect of ferulic acid against Enterobacter hormaechei and Klebsiella pneumoniae: in vitro and in silico investigation. 阿魏酸对霍氏肠杆菌和肺炎克雷伯菌的抗生物膜作用:体外和计算机实验研究。
IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-02-01 Epub Date: 2025-01-05 DOI: 10.1080/08927014.2024.2446927
Parvathi Vaikkathillam, Minsa Mini, Aparna Mohan, Devi Jayakumar, Pooja P Rajan, Sneha Asha, Praveen Kumar

Enterobacter hormaechei and Klebsiella pneumoniae, key members of the ESKAPE group of hospital-acquired pathogens, are driving forces behind numerous infections due to their potent biofilm formation and the growing threat of antimicrobial resistance. Ferulic acid (FA) is known for its strong antioxidant properties and is recognized for its numerous physiological benefits, including anti-inflammatory, antimicrobial, anticancer, and antidiabetic effects. The current investigation delves into the antimicrobial and antibiofilm ability of FA against E. hormaechei and K. pneumoniae. Using different assays, we confirmed that FA inhibits the biofilm formation of these pathogens. Through computational studies involving molecular docking and molecular dynamics simulations, it was found that FA exhibits a strong affinity for binding with MrkB in E. hormaechei and MrkH in K. pneumoniae, crucial proteins involved in biofilm formation. We hypothesise that FA might interfere with adhesion-associated molecules and inhibit biofilms through the c-di-GMP pathway and proves as an effective antibiofilm compound.

霍氏肠杆菌和肺炎克雷伯菌是医院获得性病原体ESKAPE组的主要成员,由于其强有力的生物膜形成和日益增长的抗菌素耐药性威胁,它们是许多感染背后的驱动力。阿魏酸(FA)以其强大的抗氧化特性而闻名,并被认为具有许多生理益处,包括抗炎、抗菌、抗癌和抗糖尿病作用。目前的研究深入探讨了FA对荷马氏大肠杆菌和肺炎克雷伯菌的抗菌和抗菌膜能力。通过不同的测定,我们证实FA抑制这些病原体的生物膜形成。通过分子对接和分子动力学模拟的计算研究发现,FA与E. hormaechei的MrkB和K. pneumoniae的MrkH具有很强的结合亲和力,这是参与生物膜形成的关键蛋白。我们假设FA可能通过c-di-GMP途径干扰粘附相关分子并抑制生物膜,并被证明是一种有效的抗生物膜化合物。
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Biofouling
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