Cattle killer immunoglobulin-like receptor expression on leukocyte subsets suggests functional divergence compared to humans

IF 1.4 3区农林科学 Q4 IMMUNOLOGY Veterinary immunology and immunopathology Pub Date : 2023-09-01 DOI:10.1016/j.vetimm.2023.110646

Abigail L. Hay , James Birch , Shirley Ellis , Daniel Burns , Salah Mansour , Salim I. Khakoo , John A. Hammond

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引用次数: 1

Abstract

Cattle, sheep, and goats are the only species outside primates known to have an expanded and diversified family of killer immunoglobulin-like receptors (KIR). Primate KIR are expressed on the surface of NK and T cells and bind MHC-I to control activation. However, the surface expression, ligands and function of bovid KIR remain unknown. Cattle botaKIR2DL1 is the only functional KIR of the same DL-lineage as the expanded KIR in primates and we examined if leukocyte expression patterns were consistent with human. We raised a specific mouse anti-botaKIR2DL1 monoclonal antibody and assessed its utility in flow cytometry, ELISA, and western blot. Unlike primates, cattle DL-lineage KIR (botaKIR2DL1) is present on B cells and monocytes in addition to T cells and low-level expression on NK cells. Expression decreases after in vitro PBMC stimulation with IL-2. This suggests that botaKIR2DL1 has different functions, and potentially ligands, compared to primate KIR.

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牛杀伤免疫球蛋白样受体在白细胞亚群上的表达表明与人类相比功能上存在差异

牛、绵羊和山羊是灵长类动物之外唯一已知的拥有杀手免疫球蛋白样受体（KIR）家族的物种。灵长类KIR在NK和T细胞表面表达，并与MHC-I结合以控制激活。然而，牛KIR的表面表达、配体和功能仍然未知。牛botaKIR2DL1是与灵长类动物中扩增的KIR具有相同DL谱系的唯一功能性KIR，我们检查了白细胞表达模式是否与人类一致。我们提出了一种特异性小鼠抗botaKIR2DL1单克隆抗体，并评估了其在流式细胞术、ELISA和蛋白质印迹中的实用性。与灵长类动物不同，牛DL谱系KIR（botaKIR2DL1）除了存在于T细胞和NK细胞上的低水平表达外，还存在于B细胞和单核细胞上。用IL-2刺激体外PBMC后，表达降低。这表明，与灵长类KIR相比，botaKIR2DL1具有不同的功能和潜在的配体。

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来源期刊

Veterinary immunology and immunopathology 农林科学-免疫学

CiteScore

3.40

自引率

5.60%

发文量

审稿时长

70 days

期刊介绍： The journal reports basic, comparative and clinical immunology as they pertain to the animal species designated here: livestock, poultry, and fish species that are major food animals and companion animals such as cats, dogs, horses and camels, and wildlife species that act as reservoirs for food, companion or human infectious diseases, or as models for human disease. Rodent models of infectious diseases that are of importance in the animal species indicated above,when the disease requires a level of containment that is not readily available for larger animal experimentation (ABSL3), will be considered. Papers on rabbits, lizards, guinea pigs, badgers, armadillos, elephants, antelope, and buffalo will be reviewed if the research advances our fundamental understanding of immunology, or if they act as a reservoir of infectious disease for the primary animal species designated above, or for humans. Manuscripts employing other species will be reviewed if justified as fitting into the categories above. The following topics are appropriate: biology of cells and mechanisms of the immune system, immunochemistry, immunodeficiencies, immunodiagnosis, immunogenetics, immunopathology, immunology of infectious disease and tumors, immunoprophylaxis including vaccine development and delivery, immunological aspects of pregnancy including passive immunity, autoimmuity, neuroimmunology, and transplanatation immunology. Manuscripts that describe new genes and development of tools such as monoclonal antibodies are also of interest when part of a larger biological study. Studies employing extracts or constituents (plant extracts, feed additives or microbiome) must be sufficiently defined to be reproduced in other laboratories and also provide evidence for possible mechanisms and not simply show an effect on the immune system.