Multimodal fluorescence imaging and spectroscopic techniques for oral cancer screening: a real-time approach.

IF 2.4 3区 化学 Q3 CHEMISTRY, ANALYTICAL Methods and Applications in Fluorescence Pub Date : 2023-09-13 DOI:10.1088/2050-6120/acf6ac
Pramila Thapa, Veena Singh, Sunil Bhatt, Kiran Maurya, Virendra Kumar, Vivek Nayyar, Kiran Jot, Deepika Mishra, Anurag Shrivastava, Dalip Singh Mehta
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Abstract

The survival rate of oral squamous cell carcinoma (OSCC) patients is very poor, but it can be improved using highly sensitive, specific, and accurate techniques. Autofluorescence and fluorescence techniques are very sensitive and helpful in cancer screening; being directly linked with the molecular levels of human tissue, they can be used as a quantitative tool for cancer detection. Here, we report the development of multi-modal autofluorescence and fluorescence imaging and spectroscopic (MAF-IS) smartphone-based systems for fast and real-time oral cancer screening. MAF-IS system is indigenously developed and offers the advantages of being a low-cost, handy, non-contact, non-invasive, and easily operable device that can be employed in hospitals, including low-resource settings. In this study, we report the results of 43 individuals with 28 OSCC and 15 oral potentially malignant disorders (OPMDs), i.e., epithelial dysplasia and oral submucous fibrosis, using the developed devices. We observed a red shift in fluorescence emission spectrain vivo. We found red-shift of 7.72 ± 6 nm, 3 ± 4.36 nm, and 1.33 ± 0.47 nm in the case of OSCC, epithelial dysplasia, and oral submucous fibrosis, respectively, compared to normal. The results were compared with histopathology and found to be consistent. Further, the MAF-IS system provides results in real-time with higher accuracy and sensitivity compared to devices using a single modality. Our system can achieve an accuracy of 97% with sensitivity and specificity of 100% and 94.7%, respectively, even with a smaller number of patients (28 patients of OSCC). The proposed MAF-IS device has great potential for fast screening and diagnosis of oral cancer in the future.

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口腔癌筛查的多模态荧光成像和光谱技术:一种实时方法。
口腔鳞状细胞癌(OSCC)患者的生存率很低,但使用高灵敏度、特异性和准确性的技术可以提高生存率。自体荧光和荧光技术非常敏感,有助于癌症筛查;由于与人体组织的分子水平直接相关,它们可以用作癌症检测的定量工具。在这里,我们报告了基于智能手机的多模态自体荧光和荧光成像和光谱(MAF-IS)系统的发展,用于快速和实时口腔癌筛查。MAF-IS系统是自主开发的,具有低成本、方便、非接触、非侵入性和易于操作的优点,可用于医院,包括资源匮乏的环境。在这项研究中,我们报告了43例患有28例OSCC和15例口腔潜在恶性疾病(OPMDs)(即上皮发育不良和口腔黏膜下纤维化)的患者使用该装置的结果。我们在体内观察到荧光发射光谱的红移。我们发现,与正常人相比,OSCC、上皮发育不良和口腔黏膜下纤维化的红移分别为7.72±6 nm、3±4.36 nm和1.33±0.47 nm。结果与组织病理学比较,发现是一致的。此外,与使用单一模态的设备相比,MAF-IS系统提供的实时结果具有更高的精度和灵敏度。即使在较少的患者(28例OSCC)中,我们的系统也能达到97%的准确率,100%的灵敏度和94.7%的特异性。本文提出的MAF-IS装置在口腔癌的快速筛查和诊断方面具有很大的潜力。
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来源期刊
Methods and Applications in Fluorescence
Methods and Applications in Fluorescence CHEMISTRY, ANALYTICALCHEMISTRY, PHYSICAL&n-CHEMISTRY, PHYSICAL
CiteScore
6.20
自引率
3.10%
发文量
60
期刊介绍: Methods and Applications in Fluorescence focuses on new developments in fluorescence spectroscopy, imaging, microscopy, fluorescent probes, labels and (nano)materials. It will feature both methods and advanced (bio)applications and accepts original research articles, reviews and technical notes.
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