Histological evaluation of tissue reaction and new bone formation of different calcium silicate-based cements in rats

IF 1.9 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Australian dental journal Pub Date : 2023-09-16 DOI:10.1111/adj.12980

K Bilge, O Ataş, Ş Yildiz, İ Çalik, S Dündar, A Gezer Ataş

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Abstract

Background

The purpose of this study was to evaluate the local reactions and new bone formation of rat subcutaneous and bone tissue to different calcium silicate cements.

Methods

In this study, 80 rats were divided into five groups as control, BIOfactor MTA (BIO), NeoMTA Plus (NEO), MTA Repair Hp (REP), Biodentine (DENT) and then into two subgroups according to sacrification times (7, 30 days; n = 8). Polyethylene tubes filled with appropriate materials (test groups); empty tubes (control group) were implanted into the dorsum of each rat subcutaneously. For intraosseous implantation, materials were placed in the cavities created in tibia of rats. Subcutaneous tissue and tibia samples were stained with haematoxylin-eosin and subjected to histopathological analysis. A score (0–3) was used to grade inflammatory reaction and new bone formation. Data were analysed by Kruskal–Wallis and Mann–Whitney U tests (P < 0.05).

Results

Inflammatory reaction observed in subcutaneous and intraosseous tissues for 7 days decreased significantly in all groups over time (P < 0.05). It was determined that there was significant increase in new bone formation in REP, BIO, DENT groups over time (P < 0.05).

Conclusion

Four contemporary bioceramic materials induced local inflammation and tissues changes shortly after subcutaneous implantation, which were reduced over time. In ıntraosseous implantation, all materials induced new bone formation over time.

Registration number

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不同硅酸钙基水泥对大鼠组织反应和新骨形成的组织学评估

背景：本研究旨在评估大鼠皮下和骨组织对不同硅酸钙水门汀的局部反应和新骨形成：本研究的目的是评估大鼠皮下和骨组织对不同硅酸钙水门汀的局部反应和新骨形成：本研究将 80 只大鼠分为 5 组，分别为对照组、BIOfactor MTA (BIO)、NeoMTA Plus (NEO)、MTA Repair Hp (REP)、Biodentine (DENT)，然后根据骨化时间（7 天、30 天；n = 8）分为两个亚组。将装有适当材料的聚乙烯管（试验组）和空管（对照组）植入每只大鼠的背部皮下。骨内植入时，将材料放入大鼠胫骨上的空腔中。皮下组织和胫骨样本经血苏素-伊红染色后进行组织病理学分析。对炎症反应和新骨形成进行评分（0-3 分）。数据分析采用 Kruskal-Wallis 和 Mann-Whitney U 检验（P 结果）：在皮下和骨内组织中观察到的 7 天炎症反应在所有组别中都随着时间的推移而明显减少（P 结论：四种当代生物陶瓷材料都能诱导骨形成：四种当代生物陶瓷材料在皮下植入后不久会引起局部炎症和组织变化，但随着时间的推移，炎症和组织变化会逐渐减轻。在骨外植入中，所有材料都能随着时间的推移诱导新骨形成：注册编号：ADJ-03-23-0134.© 2023 澳大利亚牙科协会。

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来源期刊

Australian dental journal 医学-牙科与口腔外科

CiteScore

4.20

自引率

4.80%

发文量

审稿时长

6-12 weeks

期刊介绍： The Australian Dental Journal provides a forum for the exchange of information about new and significant research in dentistry, promoting the discipline of dentistry in Australia and throughout the world. It comprises peer-reviewed research articles as its core material, supplemented by reviews, theoretical articles, special features and commentaries.