azyx-1 is a new gene that overlaps with zyxin and affects its translation in C. elegans, impacting muscular integrity and locomotion.

IF 7.8 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY PLoS Biology Pub Date : 2023-09-15 eCollection Date: 2023-09-01 DOI:10.1371/journal.pbio.3002300
Bhavesh S Parmar, Amanda Kieswetter, Ellen Geens, Elke Vandewyer, Christina Ludwig, Liesbet Temmerman
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Abstract

Overlapping genes are widely prevalent; however, their expression and consequences are poorly understood. Here, we describe and functionally characterize a novel zyx-1 overlapping gene, azyx-1, with distinct regulatory functions in Caenorhabditis elegans. We observed conservation of alternative open reading frames (ORFs) overlapping the 5' region of zyxin family members in several animal species, and find shared sites of azyx-1 and zyxin proteoform expression in C. elegans. In line with a standard ribosome scanning model, our results support cis regulation of zyx-1 long isoform(s) by upstream initiating azyx-1a. Moreover, we report on a rare observation of trans regulation of zyx-1 by azyx-1, with evidence of increased ZYX-1 upon azyx-1 overexpression. Our results suggest a dual role for azyx-1 in influencing zyx-1 proteoform heterogeneity and highlight its impact on C. elegans muscular integrity and locomotion.

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azyx-1是一个与zyxin重叠的新基因,影响其在秀丽隐杆线虫中的翻译,影响肌肉的完整性和运动。
重叠基因普遍存在;然而,人们对它们的表达和后果知之甚少。在这里,我们描述了一种新的zyx-1重叠基因azyx-1,并对其进行了功能表征,该基因在秀丽隐杆线虫中具有不同的调节功能。我们在几个动物物种中观察到了与zyxin家族成员5'区域重叠的选择性开放阅读框(ORF)的保守性,并在秀丽隐杆线虫中发现了azyx-1和zyxin蛋白形式表达的共享位点。与标准核糖体扫描模型一致,我们的结果支持通过上游启动azyx-1a对zyx-1长同种型的顺式调节。此外,我们报道了一个罕见的观察到的azyx-1对zyx-1的反式调节,有证据表明在azyx--1过表达时zyx-1增加。我们的研究结果表明,azyx-1在影响zyx-1蛋白型异质性方面具有双重作用,并强调了其对秀丽隐杆线虫肌肉完整性和运动的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
PLoS Biology
PLoS Biology 生物-生化与分子生物学
CiteScore
14.40
自引率
2.00%
发文量
359
审稿时长
3 months
期刊介绍: PLOS Biology is an open-access, peer-reviewed general biology journal published by PLOS, a nonprofit organization of scientists and physicians dedicated to making the world's scientific and medical literature freely accessible. The journal publishes new articles online weekly, with issues compiled and published monthly. ISSN Numbers: eISSN: 1545-7885 ISSN: 1544-9173
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