Bacteriophages potentiate the effect of antibiotics by eradication of persister cells and killing of biofilm-forming cells

IF 2.5 4区生物学 Q3 MICROBIOLOGY Research in microbiology Pub Date : 2023-09-01 DOI:10.1016/j.resmic.2023.104083

Javiera Vera-Mansilla , Cecilia A. Silva-Valenzuela , Patricio Sánchez , Roberto C. Molina-Quiroz

引用次数: 0

Abstract

Persister cells and biofilms are associated with chronic urinary infections which are more critical when generated by multi-drug resistant bacteria. In this context, joint administration of phages and antibiotics has been proposed as an alternative approach, since it may decrease the probability to generate resistant mutants to both agents. In this work, we exposed cultures of uropathogenic Escherichia coli conjunctly to antibiotics and phages. We determined that MLP2 combined with antibiotics eradicates persister cells. Similarly, MLP1 and MLP3 impact viability of biofilm-forming cells when administered with ampicillin. Our findings suggest a feasible prophylactic and therapeutic use of these non-transducing phages.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

噬菌体通过根除持久性细胞和杀死形成生物膜的细胞来增强抗生素的作用。

持久性细胞和生物膜与慢性尿路感染有关，当由耐多药细菌产生时，这种感染更为严重。在这种情况下，噬菌体和抗生素的联合给药被认为是一种替代方法，因为这可能会降低产生对这两种药物的耐药性突变体的可能性。在这项工作中，我们将尿路致病性大肠杆菌的培养物同时暴露于抗生素和噬菌体中。我们确定MLP2与抗生素联合使用可根除持久性细胞。类似地，当用氨苄青霉素给药时，MLP1和MLP3影响生物膜形成细胞的生存能力。我们的研究结果表明，使用这些非转导噬菌体进行预防和治疗是可行的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Research in microbiology 生物-微生物学

CiteScore

4.10

自引率

3.80%

发文量

审稿时长

16 days

期刊介绍： Research in Microbiology is the direct descendant of the original Pasteur periodical entitled Annales de l''Institut Pasteur, created in 1887 by Emile Duclaux under the patronage of Louis Pasteur. The Editorial Committee included Chamberland, Grancher, Nocard, Roux and Straus, and the first issue began with Louis Pasteur''s "Lettre sur la Rage" which clearly defines the spirit of the journal:"You have informed me, my dear Duclaux, that you intend to start a monthly collection of articles entitled "Annales de l''Institut Pasteur". You will be rendering a service that will be appreciated by the ever increasing number of young scientists who are attracted to microbiological studies. In your Annales, our laboratory research will of course occupy a central position, but the work from outside groups that you intend to publish will be a source of competitive stimulation for all of us."That first volume included 53 articles as well as critical reviews and book reviews. From that time on, the Annales appeared regularly every month, without interruption, even during the two world wars. Although the journal has undergone many changes over the past 100 years (in the title, the format, the language) reflecting the evolution in scientific publishing, it has consistently maintained the Pasteur tradition by publishing original reports on all aspects of microbiology.

期刊最新文献

Targeting MurG enzyme in Klebsiella pneumoniae: An in silico approach to novel antimicrobial discovery. Editorial board The genomic approach of antimicrobial resistance of Salmonella Typhimurium isolates from guinea pigs in Lima, Peru Myxobacteria: biology and bioactive secondary metabolites Archaea: current and potential biotechnological applications