Reduction of NrF2 as coadjuvant during the development of persistent periapical lesions.

C Guerrero-Bobadilla, I Yáñez-Sánchez, T Franco-Ávila, A-B Martínez-Rizo, A Domínguez-Rosales, B-A Alvarez-Rodríguez, M-E Vázquez-Sánchez, R Arias-Gómez, F-J Gálvez-Gastélum
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Abstract

Background: Persistent periapical lesions (PPL) are the result of pulpar necrosis induced by bacterial infection resulting in bone degradation and culminating with the loss of dental piece. Pathological changes in the peripapice are associated with the presence of free radicals. The transcription factor Nrf2 is the main regulator of the endogenous antioxidant response against oxidative stress and has been implicated in the regulation of osteoclastogenesis.The aim is to determine the oxidative condition in samples from patients with Persistent Periapical Injuries as a detonating factor of tissue damage.

Material and methods: An observational, descriptive, cross-sectional study was carried out in samples with PPL (cases) and samples by removal of third molars (controls) obtained in the clinic of the specialty in endodontics, University of Guadalajara. Samples were submitted to histological staining with Hematoxylin-Eosin, lipoperoxide analysis, Superoxide Dismutase (SOD), Glutathione-Peroxidase (GPx) and Catalase (CAT) activities were determined by immunoenzymatic assays and NrF2 by Western Blot analysis.

Results: Samples from PPL patients histologically showed an increased presence of lymphocytes, plasma cells, and eosinophils, as well as a decrease in extracellular matrix proteins and fibroblast cells. There was a rise in lipid peroxidation, GPx and SOD activities, but an important decline (36%) in Catalase activity was observed (p<0.005); finally, NrF2-protein was diminished at 10.41%. All comparisons were between cases vs controls.

Conclusions: The alterations in antioxidants endogenous NrF2-controlled are related to osseous destruction in patients with PPL.

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在持续性根尖周围病变的发展过程中,NrF2作为辅助剂的减少。
背景:持续性根尖周围病变(PPL)是由细菌感染引起的牙髓坏死导致骨降解并最终导致牙块丢失的结果。外皮的病理改变与自由基的存在有关。转录因子Nrf2是抗氧化应激的内源性抗氧化反应的主要调节因子,并与破骨细胞发生的调节有关。目的是确定氧化条件的样品从患者的持久性根尖周围损伤作为引爆因素的组织损伤。材料和方法:对在瓜达拉哈拉大学牙髓学专业诊所获得的PPL样本(病例)和移除第三磨牙样本(对照组)进行了一项观察性、描述性、横断面研究。样品经苏木精-伊红染色,脂过氧化物分析,免疫酶法测定超氧化物歧化酶(SOD)、谷胱甘肽-过氧化物酶(GPx)和过氧化氢酶(CAT)活性,Western Blot法测定NrF2活性。结果:PPL患者的组织学样本显示淋巴细胞、浆细胞和嗜酸性粒细胞增加,细胞外基质蛋白和成纤维细胞减少。脂质过氧化、GPx和SOD活性升高,过氧化氢酶活性明显下降(36%)(p)。结论:内源性nrf2控制的抗氧化剂的改变与PPL患者骨破坏有关。
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来源期刊
CiteScore
4.50
自引率
0.00%
发文量
52
期刊介绍: 1. Oral Medicine and Pathology: Clinicopathological as well as medical or surgical management aspects of diseases affecting oral mucosa, salivary glands, maxillary bones, as well as orofacial neurological disorders, and systemic conditions with an impact on the oral cavity. 2. Oral Surgery: Surgical management aspects of diseases affecting oral mucosa, salivary glands, maxillary bones, teeth, implants, oral surgical procedures. Surgical management of diseases affecting head and neck areas. 3. Medically compromised patients in Dentistry: Articles discussing medical problems in Odontology will also be included, with a special focus on the clinico-odontological management of medically compromised patients, and considerations regarding high-risk or disabled patients. 4. Implantology 5. Periodontology
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