Sparing and enhancing dose protraction effects for radiation damage to the aorta of wild-type mice.

IF 2.1 4区 医学 Q2 BIOLOGY International Journal of Radiation Biology Pub Date : 2024-01-01 Epub Date: 2023-08-07 DOI:10.1080/09553002.2023.2242939
Nobuyuki Hamada, Ki-Ichiro Kawano, Seiko Hirota, Yusuke Saito, Farina Mohamad Yusoff, Tatsuya Maruhashi, Makoto Maeda, Takaharu Nomura, Ayumu Nakashima, Shinji Yoshinaga, Yukihito Higashi
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引用次数: 1

Abstract

Purpose: Our previous work indicated the greater magnitude of damage to the thoracic aorta at 6 months after starting 5 Gy irradiation in descending order of exposure to X-rays in 25 fractions > acute X-rays > acute γ-rays > X-rays in 100 fractions ≫ chronic γ-rays, in which the limitations of the study included a lack of data for fractionated γ-ray exposure. To better understand effects of dose protraction and radiation quality, the present study examined changes after exposure to γ-rays in 25 fractions, and compared its biological effectiveness with five other irradiation regimens.

Materials and methods: Male C57BL/6J mice received 5 Gy of 137Cs γ-rays delivered in 25 fractions spread over six weeks. At 6 months after starting irradiation, mice were subjected to echocardiography, followed by tissue sampling. The descending thoracic aorta underwent scanning electron microscopy, immunofluorescence staining and histochemical staining. The integrative analysis of multiple aortic endpoints was conducted for inter-regimen comparisons.

Results: Exposure to γ-rays in 25 fractions induced vascular damage (evidenced by increases in endothelial detachment and vascular endothelial cell death, decreases in endothelial waviness, CD31, endothelial nitric oxide synthase and vascular endothelial cadherin), inflammation (evidenced by increases in tumor necrosis factor α, CD68 and F4/80) and fibrosis (evidenced by increases in transforming growth factor β1, alanine blue stain and intima-media thickness). The integrative analysis revealed biological effectiveness in descending order of exposure to X-rays in 25 fractions > acute X-rays > γ-rays in 25 fractions > acute γ-rays > X-rays in 100 fractions ≫ chronic γ-rays.

Conclusions: The results suggest that dose protraction effects on aortic damage depend on radiation quality, and are not a simple function of dose rate and the number of fractions.

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野生型小鼠主动脉辐射损伤的减量和增量效应
目的:我们之前的研究表明,在开始接受 5 Gy 照射 6 个月后,胸主动脉受到的损伤程度依次为 25 分段 X 射线照射 > 急性 X 射线照射 > 急性 γ 射线照射 > 100 分段 X 射线照射 ≫ 慢性 γ 射线照射,其中研究的局限性包括缺乏分段γ 射线照射的数据。为了更好地了解剂量缩短和辐射质量的影响,本研究考察了25次分段γ射线照射后的变化,并将其生物有效性与其他五种照射方案进行了比较:雄性 C57BL/6J 小鼠在六周内接受 5 Gy 的 137Cs γ 射线照射,共分 25 次照射。开始照射 6 个月后,对小鼠进行超声心动图检查,然后进行组织取样。对降胸主动脉进行扫描电子显微镜检查、免疫荧光染色和组织化学染色。对主动脉的多个终点进行了综合分析,以便在不同研究组之间进行比较:结果:暴露于 25 分量的 γ 射线会诱发血管损伤(表现为内皮脱落和血管内皮细胞死亡的增加,内皮波浪度、CD31、内皮一氧化氮合酶和血管内皮粘连蛋白的减少)、炎症(表现为肿瘤坏死因子α、CD68 和 F4/80 的增加)和纤维化(表现为转化生长因子 β1、丙氨酸蓝染色和血管内膜厚度的增加)。综合分析表明,生物效应从大到小的顺序为:暴露于 25 分段 X 射线 > 急性 X 射线 > 25 分段 γ 射线 > 急性 γ 射线 > 100 分段 X 射线 ≫ 慢性 γ 射线:结果表明,剂量缩短对主动脉损伤的影响取决于辐射质量,而不是剂量率和分段数的简单函数。
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来源期刊
CiteScore
5.00
自引率
11.50%
发文量
142
审稿时长
3 months
期刊介绍: The International Journal of Radiation Biology publishes original papers, reviews, current topic articles, technical notes/reports, and meeting reports on the effects of ionizing, UV and visible radiation, accelerated particles, electromagnetic fields, ultrasound, heat and related modalities. The focus is on the biological effects of such radiations: from radiation chemistry to the spectrum of responses of living organisms and underlying mechanisms, including genetic abnormalities, repair phenomena, cell death, dose modifying agents and tissue responses. Application of basic studies to medical uses of radiation extends the coverage to practical problems such as physical and chemical adjuvants which improve the effectiveness of radiation in cancer therapy. Assessment of the hazards of low doses of radiation is also considered.
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