Nicotine promotes Mycobacterium tuberculosis H37Rv growth and overexpression of virulence genes

IF 1.9 4区医学 Q4 IMMUNOLOGY Microbiology and Immunology Pub Date : 2023-06-28 DOI:10.1111/1348-0421.13085

Bruno Rivas-Santiago, Jeny de Haro-Acosta, Adrian Rodríguez Carlos, Mariana H. Garcia-Hernandez, Carmen J. Serrano, Irma Gonzalez-Curiel, Cesar Rivas-Santiago

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Abstract

Tobacco consumption increases the susceptibility to develop infectious diseases such as tuberculosis (TB). Nicotine (Nc) is the main component of cigarette smoke with immunomodulatory properties, however, its effect on Mycobacterium tuberculosis (Mtb) has been scarcely investigated. The present study evaluated the effect of nicotine on the growth of Mtb and on the induction of virulence-related genes. Mycobacteria were exposed to different concentrations of nicotine then Mtb growth was evaluated. Subsequently, the expression of the virulence-related genes lysX, pirG, fad26, fbpa, ompa, hbhA, esxA, esxB, hspx, katG, lpqh, and caeA was evaluated by RT-qPCR. The effect of nicotine on intracellular Mtb was also evaluated. The results showed that nicotine promotes the growth of Mtb both extracellularly and intracellularly and increases the expression of genes related to virulence. In summary, nicotine promotes the growth of Mtb and the expression of virulence-related genes that could be correlated with the increased the risk of smokers developing TB.

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尼古丁促进结核分枝杆菌H37Rv的生长和毒力基因的过表达

吸烟会增加患结核病等传染病的易感性。尼古丁是香烟烟雾中的主要成分，具有免疫调节作用，但对其对结核分枝杆菌（Mtb）的作用研究较少。本研究评估了尼古丁对结核分枝杆菌生长和毒力相关基因诱导的影响。将分枝杆菌暴露于不同浓度的尼古丁中，然后评估结核分枝杆菌的生长。随后，通过RT-qPCR评估毒力相关基因lysX、pirG、fad26、fbpa、ompa、hbhA、esxA、esxD、hspx、katG、lpqh和caeA的表达。还评估了尼古丁对细胞内Mtb的影响。结果表明，尼古丁在细胞外和细胞内都能促进结核分枝杆菌的生长，并增加与毒力相关的基因的表达。总之，尼古丁促进结核分枝杆菌的生长和毒力相关基因的表达，这些基因可能与吸烟者患结核病的风险增加有关。

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来源期刊

Microbiology and Immunology 医学-免疫学

CiteScore

5.20

自引率

3.80%

发文量

审稿时长

1 months

期刊介绍： Microbiology and Immunology is published in association with Japanese Society for Bacteriology, Japanese Society for Virology, and Japanese Society for Host Defense Research. It is peer-reviewed publication that provides insight into the study of microbes and the host immune, biological and physiological responses. Fields covered by Microbiology and Immunology include:Bacteriology|Virology|Immunology|pathogenic infections in human, animals and plants|pathogenicity and virulence factors such as microbial toxins and cell-surface components|factors involved in host defense, inflammation, development of vaccines|antimicrobial agents and drug resistance of microbes|genomics and proteomics.