Local BMP2 hydrogel therapy for robust bone regeneration in a porcine model of Legg-Calvé-Perthes disease.

IF 6.4 1区 医学 Q1 CELL & TISSUE ENGINEERING npj Regenerative Medicine Pub Date : 2023-09-14 DOI:10.1038/s41536-023-00322-2
Chi Ma, Min Sung Park, Felipe Alves do Monte, Vishal Gokani, Olumide O Aruwajoye, Yinshi Ren, Xiaohua Liu, Harry K W Kim
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引用次数: 1

Abstract

Legg-Calvé-Perthes disease is juvenile idiopathic osteonecrosis of the femoral head (ONFH) that has no effective clinical treatment. Previously, local injection of bone morphogenetic protein-2 (BMP2) for ONFH treatment showed a heterogeneous bone repair and a high incidence of heterotopic ossification (HO) due to the BMP2 leakage. Here, we developed a BMP2-hydrogel treatment via a transphyseal bone wash and subsequential injection of BMP2-loaded hydrogel. In vitro studies showed that a hydrogel of gelatin-heparin-tyramine retained the BMP2 for four weeks. The injection of the hydrogel can efficiently prevent leakage. With the bone wash, the injected hydrogel had a broad distribution in the head. In vivo studies on pigs revealed that the BMP2-hydrogel treatment produced a homogeneous bone regeneration without HO. It preserved the subchondral contour and restored the subchondral endochondral ossification, although it increased growth plate fusions. In summary, the study demonstrated a promising BMP2-hydrogel treatment for ONFH treatment, especially for teenagers.

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局部BMP2水凝胶治疗Legg-Calvé-Perthes病猪模型中强健的骨再生。
Legg-Calvé-Perthes病是一种幼年特发性股骨头坏死(ONFH),目前尚无有效的临床治疗方法。先前,局部注射骨形态发生蛋白-2(BMP2)用于ONFH治疗显示出异质性骨修复和由于BMP2渗漏导致的异位骨化(HO)的高发生率。在这里,我们开发了一种BMP2水凝胶治疗方法,通过经骨关节冲洗和随后注射负载BMP2的水凝胶。体外研究表明,明胶-肝素-酪胺的水凝胶将BMP2保留了四周。水凝胶的注射可以有效地防止渗漏。通过骨清洗,注射的水凝胶在头部有广泛的分布。对猪的体内研究表明,BMP2水凝胶处理在没有HO的情况下产生了均匀的骨再生。它保留了软骨下轮廓并恢复了软骨下软骨内骨化,尽管它增加了生长板融合。总之,该研究证明了BMP2水凝胶治疗ONFH的前景,尤其是对青少年。
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来源期刊
npj Regenerative Medicine
npj Regenerative Medicine Engineering-Biomedical Engineering
CiteScore
10.00
自引率
1.40%
发文量
71
审稿时长
12 weeks
期刊介绍: Regenerative Medicine, an innovative online-only journal, aims to advance research in the field of repairing and regenerating damaged tissues and organs within the human body. As a part of the prestigious Nature Partner Journals series and in partnership with ARMI, this high-quality, open access journal serves as a platform for scientists to explore effective therapies that harness the body's natural regenerative capabilities. With a focus on understanding the fundamental mechanisms of tissue damage and regeneration, npj Regenerative Medicine actively encourages studies that bridge the gap between basic research and clinical tissue repair strategies.
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