Time progression and regional expression of brain oxidative stress induced by obstructive jaundice in rats.

IF 2.7 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Laboratory Animal Research Pub Date : 2022-11-24 DOI:10.1186/s42826-022-00146-z

Konstantinos Lilimpakis, Aidona Tsepelaki, Electra Kalaitzopoulou, Dimitrios Zisimopoulos, Polyxeni Papadea, Marianna Skipitari, Athina Varemmenou, Apostolos Aggelis, Constantine Vagianos, Constantine Constantoyannis, Christos D Georgiou

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Abstract

Background: Obstructive jaundice induces oxidative changes in the brain parenchyma and plays significant role in clinical manifestations of hepatic encephalopathy. We aim to study the progression of the brain oxidative status over time and the differences of its pattern over the hemispheres, the brainstem and the cerebellum. We use an experimental model in rats and measuring the oxidative stress (OS) specific biomarkers protein malondialdehyde (PrMDA) and protein carbonyls (PrC = O).

Results: Hyperbilirubinemia has been confirmed in all study groups as the result of common bile duct obstruction. We confirmed increase in both PrMDA and PrC = O biomarkers levels with different type of changes over time. We also confirmed that the oxidative process develops differently in each of the brain areas in study.

Conclusions: The present study confirms the progressive increase in OS in all brain areas studied using markers indicative of cumulative protein modification.

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梗阻性黄疸大鼠脑氧化应激的时间进展及区域表达。

背景:梗阻性黄疸引起脑实质氧化改变，在肝性脑病临床表现中起重要作用。我们的目的是研究大脑氧化状态随时间的进展及其在半球、脑干和小脑上的模式差异。我们使用大鼠实验模型，测量氧化应激(OS)特异性生物标志物蛋白丙二醛(PrMDA)和蛋白羰基(PrC = O)。结果:所有研究组均证实胆总管梗阻导致高胆红素血症。我们证实了PrMDA和PrC = O生物标志物水平随时间的不同类型的变化而增加。我们还证实，在研究中，氧化过程在每个大脑区域的发展是不同的。结论:本研究证实了使用指示累积蛋白修饰的标记物研究的所有脑区OS的进行性增加。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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