Pub Date : 2026-01-30DOI: 10.1186/s42826-025-00251-9
Kyuho Kim, Ye-Jee Lee, Jae-Seung Yun, Yu-Bae Ahn, Seung-Hyun Ko
{"title":"Effects of the FXR agonist GW4064 on metabolic disorders in db/db mice.","authors":"Kyuho Kim, Ye-Jee Lee, Jae-Seung Yun, Yu-Bae Ahn, Seung-Hyun Ko","doi":"10.1186/s42826-025-00251-9","DOIUrl":"10.1186/s42826-025-00251-9","url":null,"abstract":"","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"42 1","pages":"5"},"PeriodicalIF":2.9,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12857054/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146093378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-29DOI: 10.1186/s42826-026-00266-w
Tohru Kimura
{"title":"Retraction Note: Case report on successful treatment for brain abscess in a Japanese monkey (Macaca fuscata).","authors":"Tohru Kimura","doi":"10.1186/s42826-026-00266-w","DOIUrl":"10.1186/s42826-026-00266-w","url":null,"abstract":"","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"42 1","pages":"4"},"PeriodicalIF":2.9,"publicationDate":"2026-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12853691/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146086359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Mice are useful small animal models to study the pathogenesis of SARS-CoV-2 infection. As the ancestral SARS-CoV-2 strains did not utilize murine Ace2 as a receptor, wild-type mice were not susceptible to the SARS-CoV-2 infection. Infection of human ACE2-expressing transgenic mice with SARS-CoV-2 induces fatal encephalitis, which is not commonly observed in humans. We and others have previously demonstrated the ability of the SARS-CoV-2 Beta variant to productively infect wild-type mice. Herein, we employed RNA-seq to investigate the transcriptomic landscapes in the lungs after the infection of wild-type mice with SARS-CoV-2 Beta variant.
Methods: We intranasally infected 6-week-old wild-type C57BL/6J mice with the SARS-CoV-2 (B.1.351 strain) and collected lungs at 3- and 6-days post-infection for RNA-sequencing. We used the Limma-Voom package to identify differentially expressed genes (DEGs) and the fgsea package for pathway enrichment analysis. We used Cytoscape to identify hub genes and gene networks. Lastly, we employed RT-qPCR and multiplex assay to validate the RNA-seq data.
Results: Using a cutoff of an adjusted p-value below 0.05 and an absolute log2 fold change value greater than 0.75, we identified 285 DEGs on day 3 and 46 DEGs on day 6. The canonical pathways analysis showed that several key pathways such as apoptosis and cytokine response were upregulated in the infected lungs. Protein-protein interaction analyses identified innovative target genes such as Kif11, Ccna2, and Aurkb. We also identified the top 10 hub genes that included Prc1, Ube2c, Ccnb2, Ncapg, Aurkb, Cep55, Mki67, Dlgap5, Ccna2, and Kif11. RT-qPCR analysis for Tnfa, Il6, Ccl2, and Ccl3 further validated the RNA-seq analysis. Consistent with gene expression results, we detected significantly increased protein levels of various inflammatory mediators such as IL-6, CCL2, CXCL2, and CXCL10 in the infected lungs.
Conclusions: This is the first transcriptomic analysis of the lungs of wild-type mice infected with a clinical isolate of SARS-CoV-2. Our findings provide a further understanding of the pathogenic events that occur in this mouse model of SARS-CoV-2 infection.
{"title":"Transcriptomic insights into the immune dynamics of wild-type mice challenged with SARS-CoV-2 Beta variant.","authors":"Hamid Reza Jahantigh, Amany Elsharkawy, Komal Arora, Chinonye Dim, Mukesh Kumar","doi":"10.1186/s42826-025-00264-4","DOIUrl":"10.1186/s42826-025-00264-4","url":null,"abstract":"<p><strong>Background: </strong>Mice are useful small animal models to study the pathogenesis of SARS-CoV-2 infection. As the ancestral SARS-CoV-2 strains did not utilize murine Ace2 as a receptor, wild-type mice were not susceptible to the SARS-CoV-2 infection. Infection of human ACE2-expressing transgenic mice with SARS-CoV-2 induces fatal encephalitis, which is not commonly observed in humans. We and others have previously demonstrated the ability of the SARS-CoV-2 Beta variant to productively infect wild-type mice. Herein, we employed RNA-seq to investigate the transcriptomic landscapes in the lungs after the infection of wild-type mice with SARS-CoV-2 Beta variant.</p><p><strong>Methods: </strong>We intranasally infected 6-week-old wild-type C57BL/6J mice with the SARS-CoV-2 (B.1.351 strain) and collected lungs at 3- and 6-days post-infection for RNA-sequencing. We used the Limma-Voom package to identify differentially expressed genes (DEGs) and the fgsea package for pathway enrichment analysis. We used Cytoscape to identify hub genes and gene networks. Lastly, we employed RT-qPCR and multiplex assay to validate the RNA-seq data.</p><p><strong>Results: </strong>Using a cutoff of an adjusted p-value below 0.05 and an absolute log2 fold change value greater than 0.75, we identified 285 DEGs on day 3 and 46 DEGs on day 6. The canonical pathways analysis showed that several key pathways such as apoptosis and cytokine response were upregulated in the infected lungs. Protein-protein interaction analyses identified innovative target genes such as Kif11, Ccna2, and Aurkb. We also identified the top 10 hub genes that included Prc1, Ube2c, Ccnb2, Ncapg, Aurkb, Cep55, Mki67, Dlgap5, Ccna2, and Kif11. RT-qPCR analysis for Tnfa, Il6, Ccl2, and Ccl3 further validated the RNA-seq analysis. Consistent with gene expression results, we detected significantly increased protein levels of various inflammatory mediators such as IL-6, CCL2, CXCL2, and CXCL10 in the infected lungs.</p><p><strong>Conclusions: </strong>This is the first transcriptomic analysis of the lungs of wild-type mice infected with a clinical isolate of SARS-CoV-2. Our findings provide a further understanding of the pathogenic events that occur in this mouse model of SARS-CoV-2 infection.</p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"42 1","pages":"3"},"PeriodicalIF":2.9,"publicationDate":"2026-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12849661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146093380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-23DOI: 10.1186/s42826-025-00263-5
Ji-Hun Lee, Eun-Seon Yoo, Na-Won Kim, Han-Bi Jeong, Ah-Reum Kang, Sun-Min Seo, Young-Jun Park, Byeong-Cheol Kang, Yang-Kyu Choi
{"title":"Eradication of Aspiculuris tetraptera in various immunodeficient mouse models using ivermectin: a case report.","authors":"Ji-Hun Lee, Eun-Seon Yoo, Na-Won Kim, Han-Bi Jeong, Ah-Reum Kang, Sun-Min Seo, Young-Jun Park, Byeong-Cheol Kang, Yang-Kyu Choi","doi":"10.1186/s42826-025-00263-5","DOIUrl":"10.1186/s42826-025-00263-5","url":null,"abstract":"","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"42 1","pages":"2"},"PeriodicalIF":2.9,"publicationDate":"2026-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12829027/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146030368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
<p><strong>Background: </strong>In the field of neuroscience research, executive functions (EFs) are of great significance. Patients with Parkinson's disease (PD) often encounter the problem of EFs impairment, which severely affects their lives and health. However, currently, the methods for evaluating EFs in experimental mice are limited, and there is a lack of effective evaluation indicators for touchscreen behavioral tests in different disease model mice. This study aims to establish a paradigm process and an evaluation baseline for touchscreen behavioral analysis in PD model mice, deeply explore the mechanisms of EFs impairment in PD, and provide a crucial foundation for subsequent research and treatment.</p><p><strong>Methods: </strong>Thirty clean - grade SNCA*A53T transgenic mice and forty clean - grade C57BL/6J wild - type mice were selected. For A53T mice, genetic identification was carried out. Molecular biology techniques such as PCR were used to determine their genetic characteristics. Protein detection was conducted through methods like Western blot to clarify the expression of relevant proteins. In terms of behavioral tests, the five - choice serial reaction time task (5 - CSRT) was adopted, and various behavioral data of mice in this task were recorded. Four groups were set up: the control group, the MPTP group. Different groups of mice were given specific treatments. For example, the MPTP group of mice was injected with MPTP to construct a drug - induced PD model. Principal component analysis (PCA) and receiver operating characteristic (ROC) curves were used to analyze the obtained touchscreen behavioral baseline indicators of mice, and key indicators were screened and evaluated.</p><p><strong>Results: </strong>In the 5 - CSRT, the optimal stage for wild - type C57 mice to achieve an accuracy rate of ≥ 80% was from the 11th to the 13th cycle, while for A53T mice, it was the 11th cycle. The EFs of A53T mice were impaired, with abnormalities in the accuracy rate, trace number, and number of punished times in the 5 - CSRT. When identifying drug - induced PD models, the 13th cycle of the 5 - CSRT was more effective; when identifying transgenic PD models, the 11th cycle was more suitable. Interventions could be carried out after the baseline accuracy rate reached 80%. Through the "genetic - environmental" dual - axis drive, the "chronic - acute" time - dimension complementarity, and the "mechanism - transformation" multi - level verification, the A53T and MPTP dual models were used to comprehensively cover the pathological cycle of PD EFs impairment.</p><p><strong>Conclusions: </strong>This study has successfully established a paradigm process and an evaluation baseline for touchscreen behavioral analysis in PD model mice. This provides an important basis for a deep understanding of the mechanisms of EFs impairment in PD patients, has potential guiding significance for the development of intervention strategies and treatment methods fo
{"title":"Investigate the differences in executive functions and behavioral baseline indicators of Parkinson's disease model mice based on the five - choice serial reaction time task.","authors":"Heng Gu, Zihan Liao, Zihang Zhou, Zhiyuan Liu, Mengying Gu, Xinyu Liang, Hong Pan, Chuanxi Tang","doi":"10.1186/s42826-025-00262-6","DOIUrl":"10.1186/s42826-025-00262-6","url":null,"abstract":"<p><strong>Background: </strong>In the field of neuroscience research, executive functions (EFs) are of great significance. Patients with Parkinson's disease (PD) often encounter the problem of EFs impairment, which severely affects their lives and health. However, currently, the methods for evaluating EFs in experimental mice are limited, and there is a lack of effective evaluation indicators for touchscreen behavioral tests in different disease model mice. This study aims to establish a paradigm process and an evaluation baseline for touchscreen behavioral analysis in PD model mice, deeply explore the mechanisms of EFs impairment in PD, and provide a crucial foundation for subsequent research and treatment.</p><p><strong>Methods: </strong>Thirty clean - grade SNCA*A53T transgenic mice and forty clean - grade C57BL/6J wild - type mice were selected. For A53T mice, genetic identification was carried out. Molecular biology techniques such as PCR were used to determine their genetic characteristics. Protein detection was conducted through methods like Western blot to clarify the expression of relevant proteins. In terms of behavioral tests, the five - choice serial reaction time task (5 - CSRT) was adopted, and various behavioral data of mice in this task were recorded. Four groups were set up: the control group, the MPTP group. Different groups of mice were given specific treatments. For example, the MPTP group of mice was injected with MPTP to construct a drug - induced PD model. Principal component analysis (PCA) and receiver operating characteristic (ROC) curves were used to analyze the obtained touchscreen behavioral baseline indicators of mice, and key indicators were screened and evaluated.</p><p><strong>Results: </strong>In the 5 - CSRT, the optimal stage for wild - type C57 mice to achieve an accuracy rate of ≥ 80% was from the 11th to the 13th cycle, while for A53T mice, it was the 11th cycle. The EFs of A53T mice were impaired, with abnormalities in the accuracy rate, trace number, and number of punished times in the 5 - CSRT. When identifying drug - induced PD models, the 13th cycle of the 5 - CSRT was more effective; when identifying transgenic PD models, the 11th cycle was more suitable. Interventions could be carried out after the baseline accuracy rate reached 80%. Through the \"genetic - environmental\" dual - axis drive, the \"chronic - acute\" time - dimension complementarity, and the \"mechanism - transformation\" multi - level verification, the A53T and MPTP dual models were used to comprehensively cover the pathological cycle of PD EFs impairment.</p><p><strong>Conclusions: </strong>This study has successfully established a paradigm process and an evaluation baseline for touchscreen behavioral analysis in PD model mice. This provides an important basis for a deep understanding of the mechanisms of EFs impairment in PD patients, has potential guiding significance for the development of intervention strategies and treatment methods fo","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"41 1","pages":"31"},"PeriodicalIF":2.9,"publicationDate":"2025-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12673794/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145661452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-28DOI: 10.1186/s42826-025-00260-8
Wen-Yu Chang, Tzong-Shyuan Tai, Yu-Chun Lin, Po-Han Chen, Chih-Yang Chang, Yue-Chiu Su, Ying-Hsien Kao
{"title":"Characterization of Ets-1 deficiency-induced depigmentation in a mouse model: insights into vitiligo pathogenesis.","authors":"Wen-Yu Chang, Tzong-Shyuan Tai, Yu-Chun Lin, Po-Han Chen, Chih-Yang Chang, Yue-Chiu Su, Ying-Hsien Kao","doi":"10.1186/s42826-025-00260-8","DOIUrl":"https://doi.org/10.1186/s42826-025-00260-8","url":null,"abstract":"","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"41 1","pages":"29"},"PeriodicalIF":2.9,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12661662/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145635052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-16DOI: 10.1186/s42826-025-00257-3
Hyeon Jeong Na, Yeon Su Lee, Da Eun Jung, Ji Won Seo, Jeong Su Park, Jin Woo Hong, Jae-Ho Shin
{"title":"Gastrodia elata Blume extract suppresses lipid accumulation in high-fat diet-fed rats: a biochemical and histopathological evaluation.","authors":"Hyeon Jeong Na, Yeon Su Lee, Da Eun Jung, Ji Won Seo, Jeong Su Park, Jin Woo Hong, Jae-Ho Shin","doi":"10.1186/s42826-025-00257-3","DOIUrl":"10.1186/s42826-025-00257-3","url":null,"abstract":"","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"41 1","pages":"28"},"PeriodicalIF":2.9,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12529806/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145308530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-15DOI: 10.1186/s42826-025-00258-2
Jimin Lee, Na Ahn, Sangho Roh
Background: Postapproval monitoring (PAM) is a critical component of Institutional Animal Care and Use Committee (IACUC) oversight, ensuring compliance with approved protocols and ethical animal research practices. While the PAM is not explicitly mandated under U.S. federal regulations, it has been widely recognized as an essential mechanism for verifying adherence to animal welfare standards. In contrast, Korea has formally integrated the PAM into its legal framework, making it a mandatory function of IACUCs.
Results: This study examines the implementation and perception of the PAM in Korean institutions through a survey of relevant professionals. These findings indicate that while awareness of the PAM is high, challenges such as limited manpower and institutional support hinder its effective execution. Additionally, the pandemic highlighted the potential for online remote monitoring as a supplemental method, although concerns remain regarding its effectiveness in assessing real-time animal welfare conditions. The study suggests that a hybrid PAM model, that combines onsite and remote monitoring, could improve oversight efficiency while addressing resource constraints. Strengthening administrative support, increasing professional staffing, and enhancing researcher training are crucial steps for optimizing PAM operations in Korea.
Conclusions: These insights contribute to the broader discourse on the evolution of animal research oversight and the need for adaptable monitoring strategies in diverse regulatory environments.
{"title":"The status of postapproval monitoring operation by the Institutional Animal Care and Use Committee in Korea.","authors":"Jimin Lee, Na Ahn, Sangho Roh","doi":"10.1186/s42826-025-00258-2","DOIUrl":"10.1186/s42826-025-00258-2","url":null,"abstract":"<p><strong>Background: </strong>Postapproval monitoring (PAM) is a critical component of Institutional Animal Care and Use Committee (IACUC) oversight, ensuring compliance with approved protocols and ethical animal research practices. While the PAM is not explicitly mandated under U.S. federal regulations, it has been widely recognized as an essential mechanism for verifying adherence to animal welfare standards. In contrast, Korea has formally integrated the PAM into its legal framework, making it a mandatory function of IACUCs.</p><p><strong>Results: </strong>This study examines the implementation and perception of the PAM in Korean institutions through a survey of relevant professionals. These findings indicate that while awareness of the PAM is high, challenges such as limited manpower and institutional support hinder its effective execution. Additionally, the pandemic highlighted the potential for online remote monitoring as a supplemental method, although concerns remain regarding its effectiveness in assessing real-time animal welfare conditions. The study suggests that a hybrid PAM model, that combines onsite and remote monitoring, could improve oversight efficiency while addressing resource constraints. Strengthening administrative support, increasing professional staffing, and enhancing researcher training are crucial steps for optimizing PAM operations in Korea.</p><p><strong>Conclusions: </strong>These insights contribute to the broader discourse on the evolution of animal research oversight and the need for adaptable monitoring strategies in diverse regulatory environments.</p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"41 1","pages":"27"},"PeriodicalIF":2.9,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12522620/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145301741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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