Anti-inflammatory, anticholinesterase, antioxidant, and memory enhancement potential of Phyllanthus amarus in potassium-dichromate induced neurotoxicity of male Wistar rats

IF 2.7 4区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of chemical neuroanatomy Pub Date : 2023-10-01 DOI:10.1016/j.jchemneu.2023.102308
Kingsley Afoke Iteire , Tolulope Judah Gbayisomore , Olalekan Marvelous Olatuyi
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Abstract

This study investigated the protective effect of aqueous Phyllanthus amarus leaf extract (APALE) in Potassium dichromate (PDc)-induced neurotoxicity. Seventy young adult male, Wistar rats with a weight of 130–150 g, were randomised into seven groups (n = 10): Group 1; distilled water; Group 2: 300 mg/kg APALE; Group 3: 17 mg/kg PDc; Group 4: 5 mg/kg Donepezil (DPZ); Group 5: 17 mg/kg PDc + 400 mg/kg APALE; Group 6:17 mg/kg PDc + 200 mg/kg APALE; Group 7: 17 mg/kg PDc + 5 mg/kg DPZ. All administrations were given once daily via an orogastric cannula for 28 consecutive days. Cognitive assessment tests were employed to ascertain the treatments' effects on the rats' cognitive function. At the end of the experiment, the rats were sacrificed, morphometric analysis was done, and the brains were dissected for histology, enzyme, and other biochemical analysis. Findings from this study showed that APALE significantly improved locomotive activity, recognition memory sensitivity, protection against fear and anxiety, enhanced decision-making, and improved memory function in a dose-dependent manner comparably to DPZ. In addition, APALE significantly increased antioxidants level, reducing oxidative stress in PDc-induced neurotoxic rats and significantly reducing brain acetylcholinesterase (AchE) activity by regulating gamma amino butyric acid (GABA) levels in PDc-induced neurotoxic rats compared to DPZ. Furthermore, APALE alleviated neuroinflammatory responses via maintaining histoarchitecture and down-regulation of IBA1 and Tau in PDc-induced rats. In conclusion, APALE protected against PDc-induced neurotoxicity via a combination of anti-inflammatory, anticholinergic, and antioxidant effects on the prefrontal cortex of rats.

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甘菊抗炎、抗胆碱酯酶、抗氧化和增强记忆对重铬酸钾诱导的雄性Wistar大鼠神经毒性的影响
本研究探讨了苦叶水提取物(APALE)对重铬酸钾(PDc)诱导的神经毒性的保护作用。70只体重为130–150 g的年轻成年雄性Wistar大鼠被随机分为七组(n=10):第1组;蒸馏水;第2组:300mg/kg APALE;第3组:17 mg/kg PDc;第4组:多奈哌齐5mg/kg;第5组:17 mg/kg PDc+400 mg/kg APALE;组:17 mg/kg PDc+200 mg/kg APALE;第7组:17 mg/kg PDc+5 mg/kg DPZ。所有给药均通过口胃插管每天给药一次,连续28天。采用认知评估测试来确定治疗对大鼠认知功能的影响。实验结束时,处死大鼠,进行形态计量学分析,解剖大脑进行组织学、酶和其他生化分析。这项研究的结果表明,与DPZ相比,APALE以剂量依赖的方式显著改善了机车活动、识别记忆敏感性、对恐惧和焦虑的保护、增强了决策能力,并改善了记忆功能。此外,与DPZ相比,APALE显著提高了抗氧化剂水平,降低了PDc诱导的神经毒性大鼠的氧化应激,并通过调节PDc诱导神经毒性大白鼠的γ-氨基丁酸(GABA)水平显著降低了脑乙酰胆碱酯酶(AchE)活性。此外,APALE通过维持PDc诱导的大鼠的组织结构和下调IBA1和Tau来减轻神经炎症反应。总之,APALE通过对大鼠前额叶皮层的抗炎、抗胆碱能和抗氧化作用来保护其免受PDc诱导的神经毒性。
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来源期刊
Journal of chemical neuroanatomy
Journal of chemical neuroanatomy 医学-神经科学
CiteScore
4.50
自引率
3.60%
发文量
87
审稿时长
62 days
期刊介绍: The Journal of Chemical Neuroanatomy publishes scientific reports relating the functional and biochemical aspects of the nervous system with its microanatomical organization. The scope of the journal concentrates on reports which combine microanatomical, biochemical, pharmacological and behavioural approaches. Papers should offer original data correlating the morphology of the nervous system (the brain and spinal cord in particular) with its biochemistry. The Journal of Chemical Neuroanatomy is particularly interested in publishing important studies performed with up-to-date methodology utilizing sensitive chemical microassays, hybridoma technology, immunocytochemistry, in situ hybridization and receptor radioautography, to name a few examples. The Journal of Chemical Neuroanatomy is the natural vehicle for integrated studies utilizing these approaches. The articles will be selected by the editorial board and invited reviewers on the basis of their excellence and potential contribution to this field of neurosciences. Both in vivo and in vitro integrated studies in chemical neuroanatomy are appropriate subjects of interest to the journal. These studies should relate only to vertebrate species with particular emphasis on the mammalian and primate nervous systems.
期刊最新文献
Editorial Board Retraction notice to “Astrocyte response to melatonin treatment in rats under high-carbohydrate high-fat diet” [J. Chem. Neuroanat. 136 (2024) 102389] Retraction notice to “Coenzyme Q10 attenuates neurodegeneration in the cerebellum induced by chronic exposure to tramadol” [J. Chem. Neuroanat. 135 (2024) 102367] Retraction notice to “Maternal diabetes-induced alterations in the expression of brain-derived neurotrophic factor in the developing rat hippocampus” [J. Chem. Neuroanat. 114(2021) 101946] Retraction notice to “Aqueous leaf extract of Phyllanthus amarus protects against oxidative stress and misfiring of dopaminergic neurons in Paraquat-induced Parkinson's disease-like model of adult Wistar rats” [J. Chem. Neuroanat. 135 (2024) 102365]
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