Re-identification of Colletotrichum acutatum Species Complex in Korea and Their Host Plants.

IF 1.8 3区 农林科学 Q2 PLANT SCIENCES Plant Pathology Journal Pub Date : 2023-08-01 DOI:10.5423/PPJ.OA.05.2023.0078
Le Dinh Thao, Hyorim Choi, Yunhee Choi, Anbazhagan Mageswari, Daseul Lee, Seung-Beom Hong
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Abstract

Colletotrichum acutatum species complex is one of the most important groups in the genus Colletotrichum with a high species diversity and a wide range of host plants. C. acutatum and related species have been collected from different plants and locations in Korea and deposited into the Korean Agricultural Culture Collection (KACC), National Institute of Agricultural Sciences since the 1990s. These fungal isolates were previously identified based mainly on morphological characteristics, and a limitation of molecular data was provided. To confirm the identification of species, 64 C. acutatum species complex isolates in KACC were used in this study for DNA sequence analyses of six loci: nuclear ribosomal internal transcribed spacers (ITS), betatubulin 2 (TUB2), histone-3 (HIS3), glyceraldehyde3-phosphate dehydrogenase (GAPDH), chitin synthase 1 (CHS-1), and actin (ACT). The molecular analysis revealed that they were identified in six different species of C. fioriniae (24 isolates), C. nymphaeae (21 isolates), C. scovillei (12 isolates), C. chrysanthemi (three isolates), C. lupini (two isolates), and C. godetiae (one isolate), and a novel species candidate. We compared the hosts of KACC isolates with "The List of Plant Diseases in Korea", previous reports in Korea and global reports and found that 23 combinations between hosts and pathogens could be newly reported in Korea after pathogenicity tests, and 12 of these have not been recorded in the world.

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重新鉴定韩国的 Colletotrichum acutatum 复合菌种及其寄主植物。
C. acutatum 种群是 Colletotrichum 属中最重要的种群之一,具有高度的物种多样性和广泛的寄主植物。自 20 世纪 90 年代以来,人们从韩国不同的植物和地点采集了 C. acutatum 及其相关种,并将其保存在韩国农业科学研究所的韩国农业培养物保藏中心(KACC)。以前主要根据形态特征对这些真菌分离物进行鉴定,提供的分子数据有限。为了确认物种的鉴定,本研究使用了 KACC 中的 64 个 C. acutatum 种复合体分离物进行六个位点的 DNA 序列分析:核糖体内部转录间隔(ITS)、betatubulin 2(TUB2)、组蛋白-3(HIS3)、甘油醛-3-磷酸脱氢酶(GAPDH)、几丁质合成酶 1(CHS-1)和肌动蛋白(ACT)。分子分析表明,它们在六种不同的 C. fioriniae(24 个分离株)、C. nymphaeae(21 个分离株)、C. scovillei(12 个分离株)、C. chrysanthemi(3 个分离株)、C. lupini(2 个分离株)、C. godetiae(1 个分离株)以及一个候选新种中被鉴定出来。我们将 KACC 分离物的寄主与 "韩国植物病害名录"、韩国以前的报告和全球报告进行了比较,发现经过致病性测试后,有 23 种寄主与病原体的组合可能是韩国新报告的,其中有 12 种在世界上没有记录。
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来源期刊
Plant Pathology Journal
Plant Pathology Journal 生物-植物科学
CiteScore
4.90
自引率
4.30%
发文量
71
审稿时长
12 months
期刊介绍: Information not localized
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