3D printed fluidic swab for COVID-19 testing with improved diagnostic yield and user comfort

IF 13.4 2区材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY Nano Convergence Pub Date : 2023-09-16 DOI:10.1186/s40580-023-00393-3

Joochan Kim, Jaehyung Jeon, Hyowon Jang, Youngkwang Moon, Abdurhaman Teyib Abafogi, Danny van Noort, Jinkee Lee, Taejoon Kang, Sungsu Park

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Abstract

The current standard method of diagnosing coronavirus disease 2019 (COVID-19) involves uncomfortable and invasive nasopharyngeal (NP) sampling using cotton swabs (CS), which can be unsuitable for self-testing. Although mid-turbinate sampling is an alternative, it has a lower diagnostic yield than NP sampling. Nasal wash (NW) has a similar diagnostic yield to NP sampling, but is cumbersome to perform. In this study, we introduce a 3D printed fluidic swab (3DPFS) that enables easy NW sampling for COVID-19 testing with improved diagnostic yield. The 3DPFS comprises a swab head, microchannel, and socket that can be connected to a syringe containing 250 µL of NW solution. The 3DPFS efficiently collects nasal fluid from the surface of the nasal cavity, resulting in higher sensitivity than CS for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This was confirmed by both reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and lateral flow assays (LFA) in virus-spiked nasal samples and clinical samples. Additionally, users reported greater comfort when using the 3DPFS compared to CS. These findings suggest that the 3DPFS can improve the performance of COVID-19 testing by facilitating efficient and less painful nasal sample collection.

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用于新冠肺炎检测的3D打印流体拭子，提高了诊断效率和用户舒适度。

目前诊断2019冠状病毒病（新冠肺炎）的标准方法包括使用棉签（CS）进行不舒适和侵入性鼻咽（NP）采样，这可能不适合自我检测。尽管中鼻甲取样是一种替代方法，但其诊断率低于NP取样。鼻腔冲洗（NW）具有与NP采样相似的诊断效果，但执行起来很麻烦。在这项研究中，我们介绍了一种3D打印流体拭子（3DPFS），该拭子能够轻松进行NW采样，用于新冠肺炎检测，并提高诊断率。3DPFS包括一个拭子头、微通道和插座，可以连接到含有250µL NW溶液的注射器。3DPFS有效地从鼻腔表面收集鼻腔液体，在检测严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）方面比CS具有更高的灵敏度。这一点通过逆转录定量聚合酶链式反应（RT-qPCR）和侧流分析（LFA）在掺入病毒的鼻腔样本和临床样本中得到了证实。此外，与CS相比，用户报告使用3DPFS时更舒适。这些发现表明，3DPFS可以通过促进高效和更少疼痛的鼻腔样本采集来提高新冠肺炎检测的性能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nano Convergence Engineering-General Engineering

CiteScore

15.90

自引率

2.60%

发文量

审稿时长

13 weeks

期刊介绍： Nano Convergence is an internationally recognized, peer-reviewed, and interdisciplinary journal designed to foster effective communication among scientists spanning diverse research areas closely aligned with nanoscience and nanotechnology. Dedicated to encouraging the convergence of technologies across the nano- to microscopic scale, the journal aims to unveil novel scientific domains and cultivate fresh research prospects. Operating on a single-blind peer-review system, Nano Convergence ensures transparency in the review process, with reviewers cognizant of authors' names and affiliations while maintaining anonymity in the feedback provided to authors.