Molecular detection of dengue and chikungunya viruses in surveillance of wild-caught Aedes mosquitoes in Punjab, North India.

IF 1.9 4区医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Transactions of The Royal Society of Tropical Medicine and Hygiene Pub Date : 2024-02-01 DOI:10.1093/trstmh/trad054

Taruna Kaura, Subhabrata Sarkar, Vikrant Sharma, Abhishek Mewara, Seema Devi, NazatInder Singh, Kanwalpreet Kaur, Rakesh Sehgal, R K Ratho, Gagandeep Singh Grover

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Abstract

Background: Dengue (DENV), Zika (ZIKV) and chikungunya (CHIKV) viruses are transmitted mainly by Aedes mosquitoes and are responsible for a significant global healthcare burden. The current study aimed to detect arboviruses in the Aedes mosquitoes in close proximity of patients during the transmission season.

Methods: Both immature and adult mosquitoes were collected from in and around the patients' houses. Mosquito pools were homogenized and extracted RNA was subjected to reverse transcription polymerase chain reaction for arboviral detection. Transovarian transmission (TOT) was assessed by screening F0 adults. Mosquito positivity was correlated with the aetiological agents identified in patients.

Results: Of 46 pools, 19 consisted of wild Aedes, with arboviral positivity in 53% (10/19) of pools. Among wild A. aegypti pools, positivity of DENV mono-infection, CHIKV mono-infection and DENV+CHIKV co-infection was noted in four, two and three pools, respectively. One wild pool of Aedes albopictus was positive for DENV-1. Similarly, A. aegypti F0 (adult Aedes developed from immatures) pools showed 59.2% (16/27) positivity for arboviruses. F0 Aedes showed positivity in three, six and seven pools for DENV-2, CHIKV and DENV+CHIKV, respectively, suggestive of TOT. DENV serotypes and CHIKV from 24 patients' serum samples were matched with strains isolated from Aedes and correlation was observed in four instances.

Conclusions: The study detected DENV and CHIKV from wild-caught Aedes and found evidence of DENV and CHIKV TOT in F0 adults.

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在印度北部旁遮普省对野生伊蚊的监测中进行登革热和基孔肯雅病毒的分子检测。

背景：登革热病毒（DENV）、寨卡病毒（ZIKV）和基孔肯雅病毒（CHIKV）主要由伊蚊传播，给全球医疗保健造成了巨大负担。本研究的目的是在传播季节检测患者附近伊蚊中的虫媒病毒：方法：从患者住所内和周围收集未成年蚊子和成蚊。对蚊子池进行均质，提取的 RNA 进行反转录聚合酶链反应，以检测虫媒病毒。通过筛选 F0 成蚊来评估经卵巢传播（TOT）。蚊子阳性与在患者身上发现的病原体相关：结果：在 46 个水池中，19 个水池中有野生伊蚊，53%（10/19）的水池中虫媒病毒呈阳性。在野生埃及伊蚊池中，发现单感染 DENV、单感染 CHIKV 和 DENV+CHIKV 合并感染阳性的分别有 4 个、2 个和 3 个。一个野生白纹伊蚊池对 DENV-1 呈阳性。同样，埃及伊蚊 F0（由未成年伊蚊发育而成的成年伊蚊）池对虫媒病毒的阳性率为 59.2%（16/27）。F0 伊蚊池中分别有 3 个、6 个和 7 个对 DENV-2、CHIKV 和 DENV+CHIKV 呈阳性，表明存在 TOT。24 名患者血清样本中的 DENV 血清型和 CHIKV 与从伊蚊中分离出的菌株进行了比对，发现其中 4 例存在相关性：该研究从野生捕获的伊蚊中检测到了 DENV 和 CHIKV，并在 F0 成年伊蚊中发现了 DENV 和 CHIKV TOT 的证据。

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来源期刊

Transactions of The Royal Society of Tropical Medicine and Hygiene 医学-公共卫生、环境卫生与职业卫生

CiteScore

4.00

自引率

9.10%

发文量

115

审稿时长

4-8 weeks

期刊介绍： Transactions of the Royal Society of Tropical Medicine and Hygiene publishes authoritative and impactful original, peer-reviewed articles and reviews on all aspects of tropical medicine.