Meta-Analysis of Antioxidant Mutants Reveals Common Alarm Signals for Shaping Abiotic Stress-Induced Transcriptome in Plants.

IF 5.9 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Antioxidants & redox signaling Pub Date : 2024-07-01 Epub Date: 2023-11-24 DOI:10.1089/ars.2023.0361
Shefali Mishra, Thumballi Ramabhatta Ganapathi, Girdhar Kumar Pandey, Christine Helen Foyer, Ashish Kumar Srivastava
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抗氧化突变体的荟萃分析揭示了植物非生物胁迫诱导转录组形成的共同报警信号。
目的:活性氧(Reactive oxygen species, ROS)是植物生长发育和抗逆性的关键调控因子。应激诱导的ROS水平变化触发多水平信号传导。然而,ROS信号被翻译成基因表达变化的确切机制仍然不清楚。针对六种关键的抗氧化酶,我们对公共数据库中可用的转录组数据进行了荟萃分析,以分析ros介导的核基因表达控制。结果:开发了一个信息导向的管道,确定了19个假定的转录因子(tf),作为感知ROS水平变化后的“共同报警信号级联”途径的组成部分。至关重要的是,30%-35%的非生物胁迫转录组特征在其启动子区域具有共同报警信号转录因子(cas - tf)的结合位点。此外,韧皮部早期Dof 2 (PEAR2)、DNA结合单指5.8 (DOF5.8)和obf结合蛋白3 (OBP3)被确定为顶级TFs,基于DNA亲和纯化测序(DNA亲和纯化测序)对选定基因启动子的累积DAPseq (DNA亲和纯化测序)评分,这些基因调节盐、干旱、热和冷胁迫耐受的核心途径。创新:本研究确定了一组cas - tf,它们可能在形成非生物应激诱导的ROS信号的转录组中发挥重要作用。排序分析发现,PEAR2、DOF5.8和OBP3是调节已知非生物胁迫耐受性标记的顶级cas - tf。结论:目前的研究结果表明ROS在非生物胁迫信号传导中起主要作用,并确定了一组参与信号传导的tf。综上所述,这些发现表明,共同的报警信号级联支撑了对多应力条件的大范围耐受性。鉴定相关的ros响应型cas - tf可能为作物改良提供新的靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Antioxidants & redox signaling
Antioxidants & redox signaling 生物-内分泌学与代谢
CiteScore
14.10
自引率
1.50%
发文量
170
审稿时长
3-6 weeks
期刊介绍: Antioxidants & Redox Signaling (ARS) is the leading peer-reviewed journal dedicated to understanding the vital impact of oxygen and oxidation-reduction (redox) processes on human health and disease. The Journal explores key issues in genetic, pharmaceutical, and nutritional redox-based therapeutics. Cutting-edge research focuses on structural biology, stem cells, regenerative medicine, epigenetics, imaging, clinical outcomes, and preventive and therapeutic nutrition, among other areas. ARS has expanded to create two unique foci within one journal: ARS Discoveries and ARS Therapeutics. ARS Discoveries (24 issues) publishes the highest-caliber breakthroughs in basic and applied research. ARS Therapeutics (12 issues) is the first publication of its kind that will help enhance the entire field of redox biology by showcasing the potential of redox sciences to change health outcomes. ARS coverage includes: -ROS/RNS as messengers -Gaseous signal transducers -Hypoxia and tissue oxygenation -microRNA -Prokaryotic systems -Lessons from plant biology
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