scRepli-Seq: A Powerful Tool to Study Replication Timing and Genome Instability.

IF 1.7 4区生物学 Q4 CELL BIOLOGY Cytogenetic and Genome Research Pub Date : 2022-01-01 DOI:10.1159/000527168

Megumi Sakamoto, Sakino Hori, Asahi Yamamoto, Taiki Yoneda, Kenji Kuriya, Shin-Ichiro Takebayashi

引用次数: 0

Abstract

Advances in "omics" technology have made it possible to study a wide range of cellular phenomena at the single-cell level. Recently, we developed single-cell DNA replication sequencing (scRepli-seq) that measures replication timing (RT) by copy number differences between replicated and unreplicated genomic DNA in replicating single mammalian cells. This method has been used to reveal previously unrecognized static and dynamic natures of several hundred kilobases to a few megabases-scale chromosomal units called RT domains. Because RT domains are highly correlated to A/B compartments detected by Hi-C, scRepli-seq data can be used to predict the 3D organization of the genome in the nuclear space. scRepli-seq, which essentially measures the copy number, can also be applied to study genome instability.

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scRepli-Seq:研究复制时间和基因组不稳定性的强大工具。

“组学”技术的进步使得在单细胞水平上研究广泛的细胞现象成为可能。最近，我们开发了单细胞DNA复制测序(scRepli-seq)，通过复制单个哺乳动物细胞中复制和未复制基因组DNA的拷贝数差异来测量复制时间(RT)。该方法已被用于揭示以前未被识别的数百个碱基到几兆碱基规模的染色体单位(称为RT结构域)的静态和动态特性。由于RT结构域与Hi-C检测到的A/B区室高度相关，因此scRepli-seq数据可用于预测基因组在核空间中的三维组织。scRepli-seq，本质上是测量拷贝数，也可以用于研究基因组的不稳定性。

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来源期刊

Cytogenetic and Genome Research 生物-细胞生物学

CiteScore

3.10

自引率

5.90%

发文量

审稿时长

1 months

期刊介绍： During the last decades, ''Cytogenetic and Genome Research'' has been the leading forum for original reports and reviews in human and animal cytogenetics, including molecular, clinical and comparative cytogenetics. In recent years, most of its papers have centered on genome research, including gene cloning and sequencing, gene mapping, gene regulation and expression, cancer genetics, comparative genetics, gene linkage and related areas. The journal also publishes key papers on chromosome aberrations in somatic, meiotic and malignant cells. Its scope has expanded to include studies on invertebrate and plant cytogenetics and genomics. Also featured are the vast majority of the reports of the International Workshops on Human Chromosome Mapping, the reports of international human and animal chromosome nomenclature committees, and proceedings of the American and European cytogenetic conferences and other events. In addition to regular issues, the journal has been publishing since 2002 a series of topical issues on a broad variety of themes from cytogenetic and genome research.