The effect of extracorporeal shock wave on joint capsule fibrosis in rats with knee extension contracture: a preliminary study.

IF 2.8 4区医学 Q3 CELL BIOLOGY Connective Tissue Research Pub Date : 2023-09-01 DOI:10.1080/03008207.2023.2217254

Chao Hu, Quan Bing Zhang, Feng Wang, Hua Wang, Yun Zhou

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Abstract

The purpose of this study was to observe the therapeutic effect of extracorporeal shock wave (ESW) on extensional joint contracture of knee joint in rats and its mechanism on articular capsule fibrosis. Thirty-two SD rats were randomly divided into blank control, immobilization, natural recovery, and ESW intervention groups. Except for the control group, the left knee joints of other rats were fixed with external fixation brace for 4 weeks when they were fully extended to form joint contracture. The effect of intervention was assessed by evaluating joint contracture, total cell count and collagen deposition in joint capsule, and protein expression levels of TGF-β1, p-Smad2/3, Smad2/3, p-JNK, JNK, I and III collagen in joint capsule. ESW can effectively reduce arthrogenic contracture, improve the histopathological changes of anterior joint capsule, inhibit the high expression of target protein and the excessive activation of TGF-β1/Smad2/3/JNK signal pathway. Inhibition of excessive activation of TGF-β1/Smad2/3/JNK pathway may be one of the potential molecular mechanisms by which extracorporeal shock wave can play a role.

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体外冲击波对膝关节挛缩大鼠关节囊纤维化影响的初步研究。

本研究旨在观察体外冲击波(ESW)对大鼠膝关节外张性关节挛缩的治疗作用及其对关节囊纤维化的作用机制。将32只SD大鼠随机分为空白对照组、固定组、自然恢复组和ESW干预组。除对照组外，其余大鼠左膝关节充分伸直形成关节挛缩后，采用外固定支架固定4周。通过观察关节挛缩、关节囊内总细胞计数、胶原沉积、关节囊内TGF-β1、p-Smad2/3、Smad2/3、p-JNK、JNK、I、III胶原蛋白表达水平来评价干预效果。ESW能有效减轻关节源性挛缩，改善关节前囊组织病理改变，抑制靶蛋白高表达和TGF-β1/Smad2/3/JNK信号通路的过度激活。抑制TGF-β1/Smad2/3/JNK通路的过度激活可能是体外冲击波发挥作用的潜在分子机制之一。

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来源期刊

Connective Tissue Research 生物-细胞生物学

CiteScore

6.60

自引率

3.40%

发文量

审稿时长

2 months

期刊介绍： The aim of Connective Tissue Research is to present original and significant research in all basic areas of connective tissue and matrix biology. The journal also provides topical reviews and, on occasion, the proceedings of conferences in areas of special interest at which original work is presented. The journal supports an interdisciplinary approach; we present a variety of perspectives from different disciplines, including Biochemistry Cell and Molecular Biology Immunology Structural Biology Biophysics Biomechanics Regenerative Medicine The interests of the Editorial Board are to understand, mechanistically, the structure-function relationships in connective tissue extracellular matrix, and its associated cells, through interpretation of sophisticated experimentation using state-of-the-art technologies that include molecular genetics, imaging, immunology, biomechanics and tissue engineering.