Stability of Plasma Amyloid-β 1-40, Amyloid-β 1-42, and Total Tau Protein over Repeated Freeze/Thaw Cycles.

IF 1.4 Q4 CLINICAL NEUROLOGY Dementia and Geriatric Cognitive Disorders Extra Pub Date : 2020-01-01 DOI:10.1159/000506278

Huei-Chun Liu, Ming-Jang Chiu, Chin-Hsien Lin, Shieh-Yueh Yang

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引用次数: 13

Abstract

Introduction: Blood biomarkers of Alzheimer's disease (AD) have attracted much attention of researchers in recent years. In clinical studies, repeated freeze/thaw cycles often occur and may influence the stability of biomarkers. This study aims to investigate the stability of amyloid-β 1-40 (Aβ_1-40), amyloid-β 1-42 (Aβ_1-42), and total tau protein (T-tau) in plasma over freeze/thaw cycles.

Methods: Plasma samples from healthy controls (n = 2), AD patients (AD, n =3) and Parkinson's disease patients (PD, n = 3) were collected by standardized procedure and immediately frozen at -80°C. Samples underwent 5 freeze/thaw (-80°C/room temperature) cycles. The concentrations of Aβ_1-40, Aβ_1-42, and T-tau were monitored during the freeze/thaw tests using an immunomagnetic reduction (IMR) assay. The relative percentage of concentrations after every freeze/thaw cycle was calculated for each biomarker.

Results: A tendency of decrease in the averaged relative percentages over samples through the freeze and thaw cycles for Aβ_1-40 (100 to 97.11%), Aβ_1-42 (100 to 94.99%), and T-tau (100 to 95.65%) was found. However, the decreases were less than 6%. For all three biomarkers, no statistical significance was found between the levels of fresh plasma and those of the plasma experiencing 5 freeze/thaw cycles (p > 0.1).

Conclusions: Plasma Aβ_1-40, Aβ_1-42, and T-tau are stable through 5 freeze/thaw cycles measured with IMR.

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血浆淀粉样蛋白-β 1-40、淀粉样蛋白-β 1-42和总Tau蛋白在反复冻融循环中的稳定性

近年来，阿尔茨海默病(Alzheimer's disease, AD)血液生物标志物的研究备受关注。在临床研究中，反复的冷冻/解冻循环经常发生，并可能影响生物标志物的稳定性。本研究旨在研究血浆中淀粉样蛋白-β 1-40 (Aβ1-40)、淀粉样蛋白-β 1-42 (Aβ1-42)和总tau蛋白(T-tau)在冻融循环中的稳定性。方法:按标准化程序收集健康对照(n = 2)、AD患者(n =3)和帕金森病患者(n =3)的血浆样本，并立即在-80°C冷冻。样品经过5次冷冻/解冻(-80°C/室温)循环。采用免疫磁还原(IMR)法监测冻融试验期间Aβ1-40、Aβ1-42和T-tau蛋白的浓度。计算每个生物标志物在每次冷冻/解冻循环后的相对浓度百分比。结果:通过冻融循环，Aβ1-40、Aβ1-42和T-tau的平均相对百分比呈下降趋势，分别为100 ~ 97.11%、100 ~ 94.99%和100 ~ 95.65%。然而，降幅不到6%。对于所有三种生物标志物，新鲜血浆水平与经历5次冷冻/解冻循环的血浆水平之间无统计学意义(p > 0.1)。结论:血浆a - β1-40、a - β1-42和T-tau蛋白在IMR检测的5个冻融循环中保持稳定。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Dementia and Geriatric Cognitive Disorders Extra Medicine-Psychiatry and Mental Health

CiteScore

4.30

自引率

0.00%

发文量

审稿时长

9 weeks

期刊介绍： This open access and online-only journal publishes original articles covering the entire spectrum of cognitive dysfunction such as Alzheimer’s and Parkinson’s disease, Huntington’s chorea and other neurodegenerative diseases. The journal draws from diverse related research disciplines such as psychogeriatrics, neuropsychology, clinical neurology, morphology, physiology, genetic molecular biology, pathology, biochemistry, immunology, pharmacology and pharmaceutics. Strong emphasis is placed on the publication of research findings from animal studies which are complemented by clinical and therapeutic experience to give an overall appreciation of the field. Dementia and Geriatric Cognitive Disorders Extra provides additional contents based on reviewed and accepted submissions to the main journal Dementia and Geriatric Cognitive Disorders Extra .