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{"title":"Immunophenotyping of Paucicellular Samples","authors":"Alessandra Stacchini, Anna Demurtas, Sabrina Aliberti","doi":"10.1002/0471142956.cy0946s68","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Immunophenotyping of paucicellular samples may represent a diagnostic challenge in the flow cytometry (FC) laboratory routine, as the scarcity of cells limits the number of tests that can be performed. Specimens such as fine needle aspirates (FNA), human body fluids (BF), cerebrospinal fluid (CSF), or ocular fluid (OF) sent for FC investigations in the case of suspicion of lymphoma, or for lymphoma monitoring, may contain very low numbers of cells. In these cases, it is mandatory to obtain the largest amount possible of useful information from a single tube. The basic protocol described in this unit provides a method that combines the use of multiple monoclonal antibodies (MAbs) with a Boolean gating strategy to identify and quantify the main lymphocyte populations, as well as to detect lymphomatous B cells or any aberrant T cell expression, if present, in paucicellular samples. <i>Curr. Protoc. Cytom</i>. 68:9.46.1-9.46.14. © 2014 by John Wiley & Sons, Inc.</p></div>","PeriodicalId":11020,"journal":{"name":"Current Protocols in Cytometry","volume":"68 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2018-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/0471142956.cy0946s68","citationCount":"8","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Cytometry","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/0471142956.cy0946s68","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Health Professions","Score":null,"Total":0}
引用次数: 8
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Abstract
Immunophenotyping of paucicellular samples may represent a diagnostic challenge in the flow cytometry (FC) laboratory routine, as the scarcity of cells limits the number of tests that can be performed. Specimens such as fine needle aspirates (FNA), human body fluids (BF), cerebrospinal fluid (CSF), or ocular fluid (OF) sent for FC investigations in the case of suspicion of lymphoma, or for lymphoma monitoring, may contain very low numbers of cells. In these cases, it is mandatory to obtain the largest amount possible of useful information from a single tube. The basic protocol described in this unit provides a method that combines the use of multiple monoclonal antibodies (MAbs) with a Boolean gating strategy to identify and quantify the main lymphocyte populations, as well as to detect lymphomatous B cells or any aberrant T cell expression, if present, in paucicellular samples. Curr. Protoc. Cytom . 68:9.46.1-9.46.14. © 2014 by John Wiley & Sons, Inc.
少细胞标本的免疫表型分析
由于细胞的稀缺性限制了可进行的检测的数量，因此对细胞少的样本进行免疫分型可能是流式细胞术(FC)实验室常规诊断的一个挑战。在怀疑淋巴瘤或淋巴瘤监测的情况下，将细针抽吸液(FNA)、体液(BF)、脑脊液(CSF)或眼液(OF)等标本送往FC调查，可能含有非常少的细胞。在这些情况下，必须从单个管道中获取尽可能多的有用信息。本单元描述的基本方案提供了一种方法，该方法结合使用多种单克隆抗体(mab)和布尔门控策略来识别和量化主要淋巴细胞群，以及检测淋巴瘤B细胞或任何异常T细胞表达，如果存在，在少细胞样本中。咕咕叫。Protoc。Cytom 68:9.46.1-9.46.14。©2014 by John Wiley &儿子,Inc。
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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