Anti-migratory effect of curcumin on A-549 lung cancer cells via epigenetic reprogramming of RECK/ matrix metalloproteinase axis.

IF 1.1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Hormone Molecular Biology and Clinical Investigation Pub Date : 2022-12-01 DOI:10.1515/hmbci-2021-0100

Shabnam Mostofi, Dariush Shanehbandi, Seyyed Ali Rahmani, Milad Asadi

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引用次数: 2

Abstract

Objectives: The aim of this study was to investigate the effects of curcumin on the viability, migration, and apoptosis of A549 lung cancer cells. Furthermore, RECK/MMPs axis as a probable regulator of cancer cell migration was assessed.

Methods: In this study, effect of curcumin on viability changes, cell migration, and percentage of apoptosis of A549 non-small cell lung carcinoma was examined. The methylation status of RECK gene was investigated using MS-HRM technique. Moreover, expression changes of genes involved in apoptosis and migration (including CASP3, CASP8, CASP9, BAX, BCL2, MMP9, MMP2, and RECK) were investigated by quantitative Real-Time PCR.

Results: The results of MTT assay showed that the cytotoxic effect of curcumin was in a dose dependent manner. Flow cytometry results demonstrated a significant increase in the percentage of apoptotic cells in curcumin treated group. In addition, curcumin inhibited migration rate in lung cancer cells. qRT-PCR revealed that expression of the candidate genes was in line with suppressed growth and migration. This could be due to, decreased methylation of the RECK gene promoter after curcumin treatment.

Conclusions: Curcumin inhibited lung cancer cells through various molecular pathways. RECK/MMPs axis as a regulator of cancer cell migration was modulated after curcumin treatment and invasion of lung cancer cells was decreased.

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姜黄素通过rek /基质金属蛋白酶轴的表观遗传重编程对A-549肺癌细胞的抗迁移作用

目的:研究姜黄素对肺癌A549细胞的活力、迁移和凋亡的影响。此外，RECK/MMPs轴作为癌细胞迁移的可能调节因子被评估。方法:观察姜黄素对A549非小细胞肺癌细胞活力变化、细胞迁移及凋亡率的影响。采用MS-HRM技术研究了RECK基因的甲基化状态。通过实时荧光定量PCR检测细胞凋亡和迁移相关基因(CASP3、CASP8、CASP9、BAX、BCL2、MMP9、MMP2、RECK)的表达变化。结果:MTT法显示姜黄素的细胞毒作用呈剂量依赖性。流式细胞术结果显示姜黄素处理组凋亡细胞百分比明显增加。此外，姜黄素抑制肺癌细胞的迁移速度。qRT-PCR结果显示，候选基因的表达与抑制生长和迁移一致。这可能是由于姜黄素治疗后，降低了RECK基因启动子的甲基化。结论:姜黄素通过多种分子途径抑制肺癌细胞。在姜黄素治疗后，作为癌细胞迁移调节因子的RECK/MMPs轴被调节，肺癌细胞的侵袭减少。

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来源期刊

Hormone Molecular Biology and Clinical Investigation BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

2.60

自引率

0.00%

发文量

期刊介绍： Hormone Molecular Biology and Clinical Investigation (HMBCI) is dedicated to the provision of basic data on molecular aspects of hormones in physiology and pathophysiology. The journal covers the treatment of major diseases, such as endocrine cancers (breast, prostate, endometrium, ovary), renal and lymphoid carcinoma, hypertension, cardiovascular systems, osteoporosis, hormone deficiency in menopause and andropause, obesity, diabetes, brain and related diseases, metabolic syndrome, sexual dysfunction, fetal and pregnancy diseases, as well as the treatment of dysfunctions and deficiencies. HMBCI covers new data on the different steps and factors involved in the mechanism of hormone action. It will equally examine the relation of hormones with the immune system and its environment, as well as new developments in hormone measurements. HMBCI is a blind peer reviewed journal and publishes in English: Original articles, Reviews, Mini Reviews, Short Communications, Case Reports, Letters to the Editor and Opinion papers. Ahead-of-print publishing ensures faster processing of fully proof-read, DOI-citable articles.