LncRNA colorectal neoplasia differentially expressed promotes glycolysis of liver cancer cells by regulating hypoxia-inducible factor 1α.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2022-11-01 DOI:10.4103/0304-4920.365458

Dan Tang, Lijin Zhao, Rui Mu, Yu Ao, Xuyang Zhang, Xiongxiong Li

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Abstract

LncRNAs are associated with tumorigenesis of liver cancer. LncRNA Colorectal Neoplasia Differentially Expressed (CRNDE) was identified as an oncogenic lncRNA and involved in tumor growth and metastasis. The role of CRNDE in liver cancer was investigated. CRNDE was elevated in liver cancer cells. Knockdown of CRNDE decreased cell viability and inhibited proliferation of liver cancer. Moreover, knockdown of CRNDE reduced levels of extracellular acidification rate, glucose consumption, and lactate production to repress glycolysis of liver cancer. Silence of CRNDE enhanced the expression of miR-142 and reduced enhancer of zeste homolog 2 (EZH2) and hypoxia-inducible factor 1α (HIF-1α). Over-expression of HIF-1α attenuated CRNDE silence-induced decrease of glucose consumption and lactate production. Injection with sh-CRNDE virus reduced in vivo tumor growth of liver cancer through up-regulation of miR-142 and down-regulation of EZH2 and HIF-1α. In conclusion, knockdown of CRNDE suppressed cell proliferation, glycolysis, and tumor growth of liver cancer through EZH2/miR-142/HIF-1α.

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LncRNA结直肠癌差异表达通过调控缺氧诱导因子1α促进肝癌细胞糖酵解。

lncrna与肝癌的肿瘤发生有关。结直肠癌差异表达LncRNA (Colorectal Neoplasia Differentially expression, CRNDE)是一种致癌LncRNA，参与肿瘤的生长和转移。研究了CRNDE在肝癌中的作用。肝癌细胞中CRNDE升高。下调CRNDE可降低肝癌细胞活力，抑制肝癌细胞增殖。此外，敲低CRNDE可降低细胞外酸化速率、葡萄糖消耗和乳酸生成水平，从而抑制肝癌的糖酵解。CRNDE的沉默增强了miR-142的表达，降低了zeste同源物2 (EZH2)和缺氧诱导因子1α (HIF-1α)的表达。过表达HIF-1α可减弱CRNDE沉默诱导的葡萄糖消耗和乳酸生成的减少。注射sh-CRNDE病毒可通过上调miR-142、下调EZH2和HIF-1α抑制肝癌的体内肿瘤生长。综上所述，CRNDE的下调通过EZH2/miR-142/HIF-1α抑制肝癌细胞增殖、糖酵解和肿瘤生长。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.