Plasma α-Actin as an Early Marker of Muscle Damage After Repeated Bouts of Eccentric Cycling.

IF 1.4 4区教育学 Q3 HOSPITALITY, LEISURE, SPORT & TOURISM Research Quarterly for Exercise and Sport Pub Date : 2023-09-01 DOI:10.1080/02701367.2022.2060926

Sebastian Jannas-Vela, Alvaro Bustamante, Hermann Zbinden-Foncea, Luis Peñailillo

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引用次数: 1

Abstract

Purpose: This study aimed to examine the changes in skeletal muscle (SM) α-actin, myoglobin (Mb) and hydroxyproline (HP) in plasma and other indirect markers of muscle damage after repeated bouts of eccentric cycling. Methods: Ten healthy men (23.3 ± 2.8 years) performed two 30-min eccentric cycling bouts at 100% of maximal concentric power output (230.7 ± 36.9 W) separated by 2 weeks (ECC1 and ECC2). Maximal voluntary isometric contraction (MVIC) peak force of the knee extensor muscles, muscle soreness (SOR), pain pressure threshold (PPT) and plasma levels of SM α-actin, Mb, and HP were measured before, 0.5, 3, 24-168 h after each cycling bout. Results: MVIC peak force decreased on average 10.7 ± 13.1% more after ECC1 than ECC2. SOR was 80% greater and PPT was 12-14% lower after ECC1 than ECC2. Plasma SM α-actin levels increased at 0.5, 3, and 24-72 h after ECC1 (26.1-47.9%), and SM α-actin levels at 24 h after ECC1 were associated with muscle strength loss (r = -0.56, P = .04) and SOR (r = 0.88, P = .001). Mb levels increased at 0.5, 3, and 24 h after ECC1 (200-502%). However, Mb levels at 24 h after ECC1were not associated with muscle strength loss and SOR. HP levels remained unchanged after ECC1. ECC2 did not increase SM α-actin, Mb and HP levels. Conclusion: Our results indicate that α-actin could be used as a potential marker for the early identification of SM damage due to its early appearance in plasma and its association with other indirect markers of muscle damage.

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血浆α-肌动蛋白作为反复偏心循环后肌肉损伤的早期标志。

目的:研究反复偏心循环后血浆中骨骼肌(SM) α-肌动蛋白(α-actin)、肌红蛋白(Mb)、羟脯氨酸(HP)等间接肌肉损伤指标的变化。方法:10名健康男性(23.3±2.8岁)，以100%最大同心功率输出(230.7±36.9 W)进行两次30 min偏心骑行，间隔2周(ECC1和ECC2)。分别于每次骑行前、0.5、3、24 ~ 168 h测定膝关节伸肌最大自主等距收缩力(MVIC)峰值、肌肉酸痛(SOR)、痛压阈值(PPT)及血浆SM α-肌动蛋白、Mb、HP水平。结果:ECC1术后MVIC峰值力较ECC2平均下降10.7±13.1%。与ECC2相比，ECC1术后SOR升高80%，PPT降低12-14%。ECC1术后0.5、3和24-72 h血浆SM α-actin水平升高(26.1-47.9%)，且ECC1术后24 h SM α-actin水平与肌力损失(r = -0.56, P = 0.04)和SOR (r = 0.88, P = 0.001)相关。ECC1后0.5、3和24 h, Mb水平升高(200-502%)。然而，ecc1后24小时的Mb水平与肌肉力量损失和SOR无关。ECC1后HP水平保持不变。ECC2未增加SM α-actin、Mb和HP水平。结论:α-肌动蛋白较早出现在血浆中，且与其他肌肉损伤间接标志物有关联，可作为SM损伤早期识别的潜在标志物。

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来源期刊

Research Quarterly for Exercise and Sport 社会科学-心理学

CiteScore

4.30

自引率

0.00%

发文量

125

审稿时长

6-12 weeks

期刊介绍： Research Quarterly for Exercise and Sport publishes research in the art and science of human movement that contributes significantly to the knowledge base of the field as new information, reviews, substantiation or contradiction of previous findings, development of theory, or as application of new or improved techniques. The goals of RQES are to provide a scholarly outlet for knowledge that: (a) contributes to the study of human movement, particularly its cross-disciplinary and interdisciplinary nature; (b) impacts theory and practice regarding human movement; (c) stimulates research about human movement; and (d) provides theoretical reviews and tutorials related to the study of human movement. The editorial board, associate editors, and external reviewers assist the editor-in-chief. Qualified reviewers in the appropriate subdisciplines review manuscripts deemed suitable. Authors are usually advised of the decision on their papers within 75–90 days.