Cellular toxicity profile of a new ophthalmic sponge.

IF 1.6 4区 医学 Q3 OPHTHALMOLOGY Cutaneous and Ocular Toxicology Pub Date : 2023-12-01 Epub Date: 2023-09-07 DOI:10.1080/15569527.2023.2253472
Keon Woo Park, Ji Woong Chun, Seong Won Yang, Seon Joo Park, Da Eun Jeong, Jae Woong Koh
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Abstract

Introduction: Ophthalmic sponges are used for cleaning the eye surface and absorbing fluids during ophthalmic procedures. This study compared the biological safety and stability of a new ophthalmic sponge, Occucell® (OccuTech Inc, Seongnam, Korea), on the human conjunctival epithelial cells with those of preexisting products to evaluate its clinical application.Materials and Methods: The cytotoxicity of four products, Occucell, a new product, Ultracell®, Eyetec-1, and Eyetec-2, on conjunctival epithelial cells, was evaluated using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) analysis. Additionally, human conjunctival epithelial cells were stained with a Live & Dead marker and observed using a fluorescence microscope. To evaluate the effect of the ophthalmic sponges on the secretion of IL-1β and TNF-α, cultured conjunctival epithelial cells were treated with 0.5% DMSO eluates of the ophthalmic sponges, and IL-1β and TNF-α mRNA levels were estimated using real-time polymerase chain reaction assays.Results: Cells treated with Occucell showed comparable viability to those treated with other preexisting products. Conjunctival epithelial cells showed more than 90% viability when treated with the ophthalmic sponge extracts, as determined by the MTT assay. No significant differences in the number of live & dead cells were observed between the control and treatment groups. Cells treated with all four ophthalmic sponge eluates showed similar IL-1β and TNF-α mRNA levels.Discussion: Occucell, an eye sponge used during ophthalmic surgery in clinical practice, did not affect the viability of conjunctival epithelial cells, and more than 90% of the cells were viable after the treatment. Further, Occucell showed similar effects on IL-1β and TNF-α secretion as that of other ophthalmic sponges used in the clinic. This suggested that Occucell is a safe product comparable to the preexisting products.

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一种新型眼科海绵的细胞毒性特征。
简介:在眼科手术中,眼科海绵用于清洁眼睛表面和吸收液体。本研究比较了新型眼科海绵Ococell®(韩国Seongnam,OccTech Inc)对人结膜上皮细胞的生物安全性和稳定性,并对其临床应用进行了评估。材料和方法:使用MTT(3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四唑溴化物)分析评估新产品Ultracell®、Eyetec-1和Eyetec-2对结膜上皮细胞的细胞毒性。此外,用Live&Dead标记物对人结膜上皮细胞进行染色,并使用荧光显微镜进行观察。为了评估眼用海绵对IL-1β和TNF-α分泌的影响,用眼用海绵的0.5%二甲基亚砜洗脱液处理培养的结膜上皮细胞,并使用实时聚合酶链式反应测定IL-1β、TNF-αmRNA水平。结果:用Occcell处理的细胞显示出与用其他预先存在的产物处理的细胞相当的活力。MTT法测定,当用眼海绵提取物处理时,结膜上皮细胞显示出90%以上的活力。对照组和治疗组之间未观察到活细胞和死细胞数量的显著差异。用所有四种眼部海绵洗脱液处理的细胞显示出相似的IL-1β和TNF-αmRNA水平。讨论:Occcell是临床上眼科手术中使用的一种眼部海绵,它不会影响结膜上皮细胞的生存能力,治疗后90%以上的细胞是存活的。此外,Occcell对IL-1β和TNF-α分泌的影响与临床上使用的其他眼用海绵相似。这表明Occcell是一种与现有产品相当的安全产品。
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来源期刊
CiteScore
3.30
自引率
6.20%
发文量
40
审稿时长
1 months
期刊介绍: Cutaneous and Ocular Toxicology is an international, peer-reviewed journal that covers all types of harm to cutaneous and ocular systems. Areas of particular interest include pharmaceutical and medical products; consumer, personal care, and household products; and issues in environmental and occupational exposures. In addition to original research papers, reviews and short communications are invited, as well as concise, relevant, and critical reviews of topics of contemporary significance.
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