Inhibitory effects of vorinostat (SAHA) against food-borne pathogen Salmonella enterica serotype Kentucky mixed culture biofilm with virulence and quorum-sensing relative expression.

IF 2 3区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biofouling Pub Date : 2023-05-01 Epub Date: 2023-08-14 DOI:10.1080/08927014.2023.2242263

Pantu Kumar Roy, Angela Ji-Won Ha, Shamsun Nahar, Md Iqbal Hossain, Md Ashrafudoulla, Sazzad Hossen Toushik, Md Furkanur Rahaman Mizan, Iksoon Kang, Sang-Do Ha

{"title":"Inhibitory effects of vorinostat (SAHA) against food-borne pathogen Salmonella enterica serotype Kentucky mixed culture biofilm with virulence and quorum-sensing relative expression.","authors":"Pantu Kumar Roy, Angela Ji-Won Ha, Shamsun Nahar, Md Iqbal Hossain, Md Ashrafudoulla, Sazzad Hossen Toushik, Md Furkanur Rahaman Mizan, Iksoon Kang, Sang-Do Ha","doi":"10.1080/08927014.2023.2242263","DOIUrl":null,"url":null,"abstract":"Salmonella is a food-borne microorganism that is also a zoonotic bacterial hazard in the food sector. This study determined how well a mixed culture of Salmonella Kentucky formed biofilms on plastic (PLA), silicon rubber (SR), rubber gloves (RG), chicken skin and eggshell surfaces. In vitro interactions between the histone deacetylase inhibitor-vorinostat (SAHA)-and S. enterica serotype Kentucky were examined utilizing biofilms. The minimum inhibitory concentration (MIC) of SAHA was 120 µg mL-1. The addition of sub-MIC (60 µg mL-1) of SAHA decreased biofilm formation for 24 h on PLA, SR, RG, Chicken skin, and eggshell by 3.98, 3.84, 4.11, 2.86 and 3.01 log (p < 0.05), respectively. In addition, the initial rate of bacterial biofilm formation was higher on chicken skin than on other surfaces, but the inhibitory effect was reduced. Consistent with this conclusion, virulence genes expression (avrA, rpoS and hilA) and quorum-sensing (QS) gene (luxS) was considerably downregulated at sub-MIC of SAHA. SAHA has potential as an anti-biofilm agent against S. enterica serotype Kentucky biofilm, mostly by inhibiting virulence and quorum-sensing gene expression, proving the histone deacetylase inhibitor could be used to control food-borne biofilms in the food industry.","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":"39 6","pages":"617-628"},"PeriodicalIF":2.0000,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biofouling","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/08927014.2023.2242263","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/8/14 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Salmonella is a food-borne microorganism that is also a zoonotic bacterial hazard in the food sector. This study determined how well a mixed culture of Salmonella Kentucky formed biofilms on plastic (PLA), silicon rubber (SR), rubber gloves (RG), chicken skin and eggshell surfaces. In vitro interactions between the histone deacetylase inhibitor-vorinostat (SAHA)-and S. enterica serotype Kentucky were examined utilizing biofilms. The minimum inhibitory concentration (MIC) of SAHA was 120 µg mL^-1. The addition of sub-MIC (60 µg mL^-1) of SAHA decreased biofilm formation for 24 h on PLA, SR, RG, Chicken skin, and eggshell by 3.98, 3.84, 4.11, 2.86 and 3.01 log (p < 0.05), respectively. In addition, the initial rate of bacterial biofilm formation was higher on chicken skin than on other surfaces, but the inhibitory effect was reduced. Consistent with this conclusion, virulence genes expression (avrA, rpoS and hilA) and quorum-sensing (QS) gene (luxS) was considerably downregulated at sub-MIC of SAHA. SAHA has potential as an anti-biofilm agent against S. enterica serotype Kentucky biofilm, mostly by inhibiting virulence and quorum-sensing gene expression, proving the histone deacetylase inhibitor could be used to control food-borne biofilms in the food industry.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

伏立诺司他（SAHA）对具有毒力和群体感应相对表达的食源性病原体肠道沙门氏菌血清型肯塔基混合培养生物膜的抑制作用。

沙门氏菌是一种食源性微生物，也是食品行业的人畜共患细菌危害。这项研究确定了混合培养的肯塔基沙门氏菌在塑料（PLA）、硅橡胶（SR）、橡胶手套（RG）、鸡皮和蛋壳表面形成生物膜的情况。利用生物膜检测组蛋白去乙酰化酶抑制剂伏立诺他（SAHA）-和肯塔基州血清型肠炎沙门氏菌之间的体外相互作用。SAHA的最低抑制浓度（MIC）为120 µg mL-1。添加sub-MIC（60 µg mL-1）的SAHA降低生物膜形成24 h对PLA、SR、RG、鸡皮和蛋壳的影响分别为3.98、3.84、4.11、2.86和3.01 log（p avrA、rpoS和hilA）和群体感应（QS）基因（luxS）在SAHA的亚MIC下显著下调。SAHA作为一种对抗肠炎血清型肯塔基州生物膜的抗生物膜剂具有潜力，主要通过抑制毒力和群体感应基因表达，证明组蛋白脱乙酰酶抑制剂可用于控制食品工业中的食源性生物膜。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Biofouling 生物-海洋与淡水生物学

CiteScore

5.00

自引率

7.40%

发文量

审稿时长

1.7 months

期刊介绍： Biofouling is an international, peer-reviewed, multi-discliplinary journal which publishes original articles and mini-reviews and provides a forum for publication of pure and applied work on protein, microbial, fungal, plant and animal fouling and its control, as well as studies of all kinds on biofilms and bioadhesion. Papers may be based on studies relating to characterisation, attachment, growth and control on any natural (living) or man-made surface in the freshwater, marine or aerial environments, including fouling, biofilms and bioadhesion in the medical, dental, and industrial context. Specific areas of interest include antifouling technologies and coatings including transmission of invasive species, antimicrobial agents, biological interfaces, biomaterials, microbiologically influenced corrosion, membrane biofouling, food industry biofilms, biofilm based diseases and indwelling biomedical devices as substrata for fouling and biofilm growth, including papers based on clinically-relevant work using models that mimic the realistic environment in which they are intended to be used.