The complete genome sequence of Pseudomonas chengduensis BC1815 for genome mining of PET degrading enzymes

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2023-02-01 DOI:10.1016/j.margen.2022.101008
Zhengguang Shi , Xue Yu , Jingjing Duan , Wenbin Guo
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引用次数: 2

Abstract

Pseudomonas chengduensis BC1815, isolated from a marine sediment sample of the Pacific Ocean, can grow in mineral medium with PET plastic as sole carbon source. Here, we present the complete genome of Pseudomonas chengduensis BC1815, which will facilitate the genome mining of PET degrading enzymes. The total length of the sequenced genome consists of 5,578,440 bases, with mean G + C content of 62.65%. A total of 5150 coding genes including 65 tRNAs and 12 rRNAs were predicted in the genome. Thirteen proteins of esterase, lipase and α/β hydrolase were taken as candidates for PET degrading enzymes, in which eight were membrane bounded and the others were secretory.

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成都假单胞菌BC1815的全基因组序列,用于PET降解酶的基因组挖掘
从太平洋海洋沉积物样品中分离出的成都假单胞菌BC1815可在以PET塑料为唯一碳源的矿物介质中生长。在此,我们提出了成都假单胞菌BC1815的完整基因组,这将有助于PET降解酶的基因组挖掘。测序的基因组总长度为5,578,440个碱基,平均G + C含量为62.65%。在基因组中共预测到5150个编码基因,包括65个trna和12个rnas。选取了酯酶、脂肪酶和α/β水解酶的13种蛋白作为PET降解酶的候选蛋白,其中8种为膜结合蛋白,其余为分泌蛋白。
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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