Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) RNA in the Human Eye.

IF 2.6 4区医学 Q2 OPHTHALMOLOGY Ocular Immunology and Inflammation Pub Date : 2023-01-01 DOI:10.1080/09273948.2021.1980810

Alireza Kamalipour, Mohammad Ali Ashraf, Sasan Moghimi, Afagh Moattari, Mohammad Javad Ashraf, Farhad Abbasi, Farzan Azodi, Shadi Oboudi, Neda Pirbonyeh, Maryam Mokhtaryan, Amirhossein Roshanshad, Jiun L Do, Robert N Weinreb

{"title":"Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) RNA in the Human Eye.","authors":"Alireza Kamalipour, Mohammad Ali Ashraf, Sasan Moghimi, Afagh Moattari, Mohammad Javad Ashraf, Farhad Abbasi, Farzan Azodi, Shadi Oboudi, Neda Pirbonyeh, Maryam Mokhtaryan, Amirhossein Roshanshad, Jiun L Do, Robert N Weinreb","doi":"10.1080/09273948.2021.1980810","DOIUrl":null,"url":null,"abstract":"Purpose: To determine the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in postmortem ocular specimens of patients with severe COVID-19 disease.Patients and methods: Postmortem conjunctival (28 samples), aqueous humor (30 samples) and vitreous humor (30 samples) specimens were obtained bilaterally from the eyes of 15 deceased COVID-19 patients within one hour of death. The presence of viral RNA was evaluated in samples using Real-time reverse transcriptase-polymerase chain reaction (RT-PCR).Results: Positive RT-PCR SARS-COV-2 results were found in one conjunctival and 2 vitreous humor samples. All aqueous humor samples tested negative for the presence of SARS-COV-2 RNA. Of note, three positive samples were obtained from three different patients. The overall prevalence of positive RT-PCR ocular samples was 3.4% among all samples and 20% at the patient level.Conclusion: SARS-CoV-2 RNA is detectable in postmortem conjunctival and vitreous humor samples of patients with severe COVID-19.","PeriodicalId":19406,"journal":{"name":"Ocular Immunology and Inflammation","volume":"31 1","pages":"32-38"},"PeriodicalIF":2.6000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ocular Immunology and Inflammation","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/09273948.2021.1980810","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"OPHTHALMOLOGY","Score":null,"Total":0}

引用次数: 1

Abstract

Purpose: To determine the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in postmortem ocular specimens of patients with severe COVID-19 disease.

Patients and methods: Postmortem conjunctival (28 samples), aqueous humor (30 samples) and vitreous humor (30 samples) specimens were obtained bilaterally from the eyes of 15 deceased COVID-19 patients within one hour of death. The presence of viral RNA was evaluated in samples using Real-time reverse transcriptase-polymerase chain reaction (RT-PCR).

Results: Positive RT-PCR SARS-COV-2 results were found in one conjunctival and 2 vitreous humor samples. All aqueous humor samples tested negative for the presence of SARS-COV-2 RNA. Of note, three positive samples were obtained from three different patients. The overall prevalence of positive RT-PCR ocular samples was 3.4% among all samples and 20% at the patient level.

Conclusion: SARS-CoV-2 RNA is detectable in postmortem conjunctival and vitreous humor samples of patients with severe COVID-19.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

严重急性呼吸综合征冠状病毒2 (SARS-CoV-2) RNA在人眼中的检测

目的:检测重症COVID-19患者死后眼部标本中是否存在SARS-CoV-2 RNA。患者和方法:从15例COVID-19死亡患者死亡1小时内双侧眼采集死后结膜标本(28份)、房水标本(30份)和玻璃体标本(30份)。采用实时逆转录聚合酶链反应(RT-PCR)评估样品中病毒RNA的存在。结果:1例结膜标本和2例玻璃体标本RT-PCR检测SARS-COV-2阳性。所有房水样品的SARS-COV-2 RNA检测均为阴性。值得注意的是，三个阳性样本来自三个不同的患者。在所有样本中，RT-PCR阳性眼部样本的总体患病率为3.4%，在患者水平上为20%。结论:在重症COVID-19患者死后结膜和玻璃体样本中检测到SARS-CoV-2 RNA。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Ocular Immunology and Inflammation 医学-眼科学

CiteScore

6.20

自引率

15.20%

发文量

285

审稿时长

6-12 weeks

期刊介绍： Ocular Immunology & Inflammation ranks 18 out of 59 in the Ophthalmology Category.Ocular Immunology and Inflammation is a peer-reviewed, scientific publication that welcomes the submission of original, previously unpublished manuscripts directed to ophthalmologists and vision scientists. Published bimonthly, the journal provides an international medium for basic and clinical research reports on the ocular inflammatory response and its control by the immune system. The journal publishes original research papers, case reports, reviews, letters to the editor, meeting abstracts, and invited editorials.