Polydactyly-derived allogeneic chondrocyte cell-sheet transplantation with high tibial osteotomy as regenerative therapy for knee osteoarthritis.

IF 6.4 1区医学 Q1 CELL & TISSUE ENGINEERING npj Regenerative Medicine Pub Date : 2022-12-16 DOI:10.1038/s41536-022-00272-1

Kosuke Hamahashi, Eriko Toyoda, Miya Ishihara, Genya Mitani, Tomonori Takagaki, Nagatoshi Kaneshiro, Miki Maehara, Takumi Takahashi, Eri Okada, Ayako Watanabe, Yoshihiko Nakamura, Reiko Kato, Ryo Matoba, Takehiko Takagi, Hidenori Akutsu, Akihiro Umezawa, Hiroyuki Kobayashi, Tadashi Akamatsu, Masayuki Yamato, Teruo Okano, Masahiko Watanabe, Masato Sato

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引用次数: 5

Abstract

Allogeneic cell therapies are not fully effective in treating osteoarthritis of the knee (OAK). We recently reported that transplantation of autologous chondrocyte cell-sheets along with open-wedge high tibial osteotomy promoted hyaline cartilage repair in humans. Here we describe our regenerative therapy for OAK using polydactyly-derived allogeneic chondrocyte cell-sheets (PD sheets) and temperature-responsive culture inserts. Ten patients with OAK and cartilage defects categorized arthroscopically as Outerbridge grade III or IV received the therapy. Cartilage viscoelasticity and thickness were assessed before and after transplantation. Arthroscopic biopsies obtained 12 months after transplantation were analyzed histologically. Gene expression was analyzed to evaluate the PD sheets. In this small initial longitudinal series, PD sheet transplantation was effective in treating OAK, as indicated by changes in cartilage properties. Gene marker sets in PD sheets may predict outcomes after therapy and provide markers for the selection of donor cells. This combined surgery may be an ideal regenerative therapy with disease-modifying effects in OAK patients.

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多趾异体软骨细胞片移植联合胫骨高位截骨作为膝关节骨关节炎的再生治疗。

同种异体细胞疗法在治疗膝关节骨关节炎(OAK)方面并不完全有效。我们最近报道了自体软骨细胞片移植和开楔胫骨高位截骨术促进了人类透明软骨的修复。在这里，我们描述了我们使用多指衍生异体软骨细胞片(PD片)和温度响应培养插入物对OAK的再生治疗。10例经关节镜分类为Outerbridge III级或IV级的OAK和软骨缺损患者接受了治疗。观察移植前后软骨的粘弹性和厚度。移植后12个月的关节镜活检进行组织学分析。通过分析基因表达来评价PD片。在这个小的初始纵向系列中，PD片移植治疗OAK是有效的，正如软骨特性的改变所表明的那样。PD表中的基因标记集可以预测治疗后的结果，并为供体细胞的选择提供标记。这种联合手术可能是一种理想的再生治疗，对OAK患者具有改善疾病的作用。

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来源期刊

npj Regenerative Medicine Engineering-Biomedical Engineering

CiteScore

10.00

自引率

1.40%

发文量

审稿时长

12 weeks

期刊介绍： Regenerative Medicine, an innovative online-only journal, aims to advance research in the field of repairing and regenerating damaged tissues and organs within the human body. As a part of the prestigious Nature Partner Journals series and in partnership with ARMI, this high-quality, open access journal serves as a platform for scientists to explore effective therapies that harness the body's natural regenerative capabilities. With a focus on understanding the fundamental mechanisms of tissue damage and regeneration, npj Regenerative Medicine actively encourages studies that bridge the gap between basic research and clinical tissue repair strategies.