Effect of fermentation pH on the structure, rheological properties, and antioxidant activities of exopolysaccharides produced by Alteromonas australica QD.

IF 2.7 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Glycoconjugate Journal Pub Date : 2022-12-01 DOI:10.1007/s10719-022-10087-3

Fengshu Li, Xin Hu, Xiaojun Sun, Haoshuai Li, Jinren Lu, Yiming Li, Mutai Bao

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引用次数: 2

Abstract

The pH value was essential for the growth and metabolism of microorganisms. Acidic pH exopolysaccharide (AC-EPS) and alkaline pH exopolysaccharide (AL-EPS) secreted by A. australica QD mediated by pH were studied in this paper. The total carbohydrate content and molecular weight of AC-EPS (79.59% ± 2.24% (w/w), 8.374 × 10⁵ Da) and AL-EPS (82.48% ± 1.46% (w/w), 6.182 × 10⁵ Da) were estimated and compared. In AC-EPS, mannose (3.78%) and galactose (3.24%) content was more, while the proportion of glucuronic acid was less in comparison to AL-EPS. The scanning electron microscopy revealed the structural differences among the AC-EPS and AL-EPS. Thermogravimetric analysis showed degradation temperatures of 272.8 °C and 244.9 °C for AC-EPS and AL-EPS, respectively. AC-EPS was found to exhibit better rheological properties and emulsifying capabilities, while AL-EPS had superior antioxidant activities. Overall, both AC-EPS and AL-EPS have the potential to be used as emulsifiers and biological antioxidants.

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发酵pH对澳大利亚异单胞菌胞外多糖结构、流变学特性及抗氧化活性的影响

pH值是微生物生长和代谢所必需的。研究了酸碱度介导的澳洲木质素QD分泌的酸性pH外多糖(AC-EPS)和碱性pH外多糖(AL-EPS)。比较了AC-EPS的总碳水化合物含量(79.59%±2.24% (w/w)， 8.374 × 105 Da)和AL-EPS的总碳水化合物含量(82.48%±1.46% (w/w)， 6.182 × 105 Da)。AC-EPS中甘露糖(3.78%)和半乳糖(3.24%)的含量高于AL-EPS，葡萄糖醛酸的含量低于AL-EPS。扫描电镜显示了AC-EPS和AL-EPS的结构差异。热重分析表明，AC-EPS和AL-EPS的降解温度分别为272.8℃和244.9℃。AC-EPS具有较好的流变性能和乳化性能，而AL-EPS具有较好的抗氧化活性。综上所述，AC-EPS和AL-EPS都具有作为乳化剂和生物抗氧化剂的潜力。

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来源期刊

Glycoconjugate Journal 生物-生化与分子生物学

CiteScore

6.00

自引率

3.30%

发文量

审稿时长

1 months

期刊介绍： Glycoconjugate Journal publishes articles and reviews on all areas concerned with: function, composition, structure, biosynthesis, degradation, interactions, recognition and chemo-enzymatic synthesis of glycoconjugates (glycoproteins, glycolipids, oligosaccharides, polysaccharides and proteoglycans), biochemistry, molecular biology, biotechnology, immunology and cell biology of glycoconjugates, aspects related to disease processes (immunological, inflammatory, arthritic infections, metabolic disorders, malignancy, neurological disorders), structural and functional glycomics, glycoimmunology, glycovaccines, organic synthesis of glycoconjugates and the development of methodologies if biologically relevant, glycosylation changes in disease if focused on either the discovery of a novel disease marker or the improved understanding of some basic pathological mechanism, articles on the effects of toxicological agents (alcohol, tobacco, narcotics, environmental agents) on glycosylation, and the use of glycotherapeutics. Glycoconjugate Journal is the official journal of the International Glycoconjugate Organization, which is responsible for organizing the biennial International Symposia on Glycoconjugates.