The genome of the mustard hill coral, Porites astreoides.

GigaByte (Hong Kong, China) Pub Date : 2022-07-29 eCollection Date: 2022-01-01 DOI:10.46471/gigabyte.65
Kevin H Wong, Hollie M Putnam
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Abstract

Anthropogenic effects have contributed to substantial declines in coral reefs worldwide. However, some corals are more resilient to environmental changes and have increased in relative abundance, thus these species may shape future reef communities. Here, we provide the first draft reference genome for the mustard hill coral, Porites astreoides, collected in Bermuda. DNA was sequenced via Pacific Biosciences (PacBio) HiFi long-read technology. PacBio read assembly with FALCON UnZip resulted in a 678-Mbp assembly with 3051 contigs with an N50 of 412,256 and the BUSCO completeness analysis resulted in 90.9% of the metazoan gene set. An ab initio transcriptome was also produced with 64,636 gene models with a transcriptome BUSCO completeness analysis of 77.5% versus the metazoan gene set. Functional annotation was completed for 86.6% of proteins. These data are valuable resources for improving biological knowledge of P. astreoides, facilitating comparative genomics for corals, and supporting evidence-based restoration and human-assisted evolution of corals.

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芥子山珊瑚(Porites astreoides)的基因组。
人类活动造成全球珊瑚礁数量大幅减少。然而,一些珊瑚对环境变化的适应能力更强,相对数量也有所增加,因此这些物种可能会塑造未来的珊瑚礁群落。在此,我们提供了在百慕大采集的芥子山珊瑚(Porites astreoides)的第一个参考基因组草案。DNA 测序采用太平洋生物科学公司(PacBio)的 HiFi 长读取技术。使用 FALCON UnZip 对 PacBio 读数进行组装后,得到了 678-Mbp 的组装结果,其中包含 3051 个 N50 为 412,256 的等位基因,BUSCO 完整性分析结果显示,元古代基因组占 90.9%。此外,还产生了一个包含 64,636 个基因模型的 ab initio 转录组,其转录组 BUSCO 完整性分析结果为元动物基因组的 77.5%。86.6%的蛋白质完成了功能注释。这些数据是宝贵的资源,有助于提高对 P. astreoides 的生物学认识,促进珊瑚的比较基因组学研究,并支持珊瑚的循证修复和人类辅助进化。
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来源期刊
CiteScore
2.60
自引率
0.00%
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0
审稿时长
5 weeks
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