Evaluation of Therapeutic Potential of Selected Plant-Derived Homeopathic Medicines for their Action against Cervical Cancer.

IF 1.2 4区医学 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Homeopathy Pub Date : 2023-11-01 Epub Date: 2023-03-01 DOI:10.1055/s-0042-1756436

Tejveer Singh, Nikita Aggarwal, Kulbhushan Thakur, Arun Chhokar, Joni Yadav, Tanya Tripathi, Mohit Jadli, Anjali Bhat, Arun Kumar, Ritika Hasija Narula, Pankaj Gupta, Anil Khurana, Alok Chandra Bharti

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Abstract

Background: Plant-derived homeopathic medicines (HMs) are cheap and commercially available but are mechanistically less explored entities than conventional medicines.

Purpose: The aim of our study was to evaluate the impact of selected plant-derived HMs derived from Berberis aquifolium (BA), Berberis vulgaris (BV), Mentha piperita (MP), Curcuma longa (CL), Cinchona officinalis (CO), Thuja occidentalis (TO) and Hydrastis canadensis (HC) on cervical cancer (CaCx) cells in vitro.

Methods: We screened the mother tincture (MT) and 30C potencies of the above-mentioned HMs for anti-proliferative and cytotoxic activity on human papillomavirus (HPV)-negative (C33a) and HPV-positive CaCx cells (SiHa and HeLa) by MTT assay. Total phenolic content (TPC) and the free-radical scavenging activity of each HM was also determined using standard assays. Phytochemicals reportedly available in these HMs were examined for their potential inhibitory action on HPV16 E6 by in silico molecular docking.

Results: All tested MTs induced a differential dose-dependent cytotoxic response that varied with cell line. For C33a cells, the order of response was TO > CL > BA > BV > HC > MP > CO, whereas for SiHa and HeLa cells the order was HC > MP > TO > CO > BA > BV > CL and CL > BA > CO, respectively. 30C potencies of all HMs showed an inconsistent response. Further, anti-CaCx responses displayed by MTs did not follow the order of an HM's phenolic content or free radical scavenging activity. Analysis revealed anti-oxidant content of BA, BV and HC had the lowest contribution to their anti-CaCx activity. Using in silico modeling of molecular docking between the HPV16 E6 protein crystallographic structures (6SJA and 4XR8) and main phytochemical components of BV, BA, HC, CL and TO, their potential to inhibit the HPV16 E6 protein carcinogenic interactions was identified.

Conclusion: The study has shown a comparative evaluation of the potential of several plant-derived MTs and HMs to affect CaCx cell line survival in vitro (through cytotoxicity and free radical scavenging) and their theoretical molecular targets in silico for the first time. Data demonstrated that MTs of BA and BV are likely to be the most potent HMs that strongly inhibited CaCx growth and have a strong anti-HPV phytochemical constitution.

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选定的植物衍生顺势疗法药物对癌症的治疗潜力评估。

背景：植物来源的顺势疗法药物（HM）价格低廉，可在商业上获得，但在机制上比传统药物探索较少。目的：本研究的目的是评价从小檗（BA）、小檗属（BV）、薄荷属（MP）、姜黄属（CL）、金鸡纳（CO）、洋地黄属（to）和加拿大水草属（HC）中提取的植物来源的HMs对体外培养的癌症（CaCx）细胞的影响。方法：我们通过MTT法筛选了上述HMs的母酊（MT）和30C对人乳头瘤病毒（HPV）阴性（C33a）和HPV阳性CaCx细胞（SiHa和HeLa）的抗增殖和细胞毒性活性。还使用标准测定法测定了每种HM的总酚含量（TPC）和自由基清除活性。据报道，这些HM中可用的植物化学物质通过硅分子对接检测了其对HPV16 E6的潜在抑制作用。结果：所有测试的MT都诱导了不同的剂量依赖性细胞毒性反应，该反应随细胞系的不同而变化。对于C33a细胞，反应顺序为TO > CL > BA > BV > HC > MP > CO，而对于SiHa和HeLa细胞，顺序为HC > MP > 至 > CO > BA > BV > CL和CL > BA > CO。所有HM的30C潜能显示出不一致的反应。此外，MT显示的抗CaCx反应不遵循HM的酚类含量或自由基清除活性的顺序。分析表明，BA、BV和HC的抗氧化含量对其抗CaCx活性的贡献最小。利用HPV16 E6蛋白结晶结构（6SJA和4XR8）与BV、BA、HC、CL和TO的主要植物化学成分之间的分子对接的计算机模拟，确定了它们抑制HPV16 E6蛋白致癌相互作用的潜力。结论：该研究首次在计算机上对几种植物来源的MT和HM影响CaCx细胞系体外存活的潜力（通过细胞毒性和自由基清除）及其理论分子靶标进行了比较评估。数据表明，BA和BV的MT可能是最有效的HM，它们强烈抑制CaCx生长，并具有强烈的抗HPV植物化学成分。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Homeopathy 医学-全科医学与补充医学

CiteScore

3.40

自引率

70.60%

发文量

审稿时长

20.1 weeks

期刊介绍： Homeopathy is an international peer-reviewed journal aimed at improving the fundamental understanding and clinical practice of homeopathy by publishing relevant high-quality original research articles, reviews, and case reports. It also promotes commentary and debate on matters of topical interest in homeopathy.