Chromosomal damage in occupationally exposed health professionals assessed by two cytogenetic methods.

IF 1.4 4区医学 Q4 ENVIRONMENTAL SCIENCES Archives of Environmental & Occupational Health Pub Date : 2023-01-01 DOI:10.1080/19338244.2022.2118213

Dita Kadlcikova, Petra Musilova, Hana Hradska, Miluse Vozdova, Marketa Petrovova, Marek Svoboda, Jiri Rubes

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Abstract

The study assessed occupationally induced chromosomal damage in hospital personnel at risk of exposure to antineoplastic drugs and/or low doses of ionizing radiation by two cytogenetic methods. Cultured peripheral blood lymphocytes of eighty-five hospital workers were examined twice over 2 to 3 years by classical chromosomal aberration analysis and fluorescence in situ hybridization. The comparison of the 1^st and the 2^nd sampling of hospital workers showed a significant increase in chromatid and chromosomal aberrations (all p < .05) examined by classical chromosomal aberration analysis, and in unstable aberrations (all p < .05) detected by fluorescence in situ hybridization. Both cytogenetic methods were able to detect an increase of unstable aberrations in the 2^nd sampling. The raised frequency of unstable cytogenetic parameters suggested higher recent exposure to genotoxic agents.

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用两种细胞遗传学方法评估职业暴露卫生专业人员的染色体损伤。

本研究通过两种细胞遗传学方法评估了有暴露于抗肿瘤药物和/或低剂量电离辐射风险的医院工作人员的职业性染色体损伤。本文采用经典染色体畸变法和荧光原位杂交法对85例医院职工体外培养的外周血淋巴细胞进行了2 ~ 3年的检测。第1次和第2次抽样比较，医务工作者的染色单体和染色体畸变明显增加(均为原位杂交)。两种细胞遗传学方法都能够在第二次采样中检测到不稳定畸变的增加。不稳定细胞遗传学参数的频率增加表明最近暴露于遗传毒性物质较多。

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来源期刊

Archives of Environmental & Occupational Health 环境科学-公共卫生、环境卫生与职业卫生

CiteScore

3.70

自引率

0.00%

发文量

审稿时长

>36 weeks

期刊介绍： Archives of Environmental & Occupational Health , originally founded in 1919 as the Journal of Industrial Hygiene, and perhaps most well-known as the Archives of Environmental Health, reports, integrates, and consolidates the latest research, both nationally and internationally, from fields germane to environmental health, including epidemiology, toxicology, exposure assessment, modeling and biostatistics, risk science and biochemistry. Publishing new research based on the most rigorous methods and discussion to put this work in perspective for public health, public policy, and sustainability, the Archives addresses such topics of current concern as health significance of chemical exposure, toxic waste, new and old energy technologies, industrial processes, and the environmental causation of disease such as neurotoxicity, birth defects, cancer, and chronic degenerative diseases. For more than 90 years, this noted journal has provided objective documentation of the effects of environmental agents on human and, in some cases, animal populations and information of practical importance on which decisions are based.