{"title":"L-Glutamate-glyoxylate aminotransferase in Lactobacillus plantarum.","authors":"E Galas, T Florianowicz","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Glutamate-glyoxylate aminotransferase which mediates the reaction of glyoxylic acid with glutamic acid to yield glycine and alpha-oxoglutaric acid has been isolated and purified 84-fold from extracts of Lactobacillus plantarum. Purified enzyme requires the addition of pyridoxal phosphate and magnesium ions for its activity. The molecular weight of the enzyme estimated by Sepharose 4B gel filtration amounts to 37.000. Micaelis constants for glyoxylate and glutamate are corresponding to 6.25 X 10(-3) M and 2.75 X 10(-3) M, respectively. Optimal pH in phosphate and veronal buffers is 8.0 and optimal temperature 35--37 degrees C.</p>","PeriodicalId":75390,"journal":{"name":"Acta microbiologica Polonica. Series B: Microbiologia applicata","volume":"7 4","pages":"243-52"},"PeriodicalIF":0.0000,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta microbiologica Polonica. Series B: Microbiologia applicata","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Glutamate-glyoxylate aminotransferase which mediates the reaction of glyoxylic acid with glutamic acid to yield glycine and alpha-oxoglutaric acid has been isolated and purified 84-fold from extracts of Lactobacillus plantarum. Purified enzyme requires the addition of pyridoxal phosphate and magnesium ions for its activity. The molecular weight of the enzyme estimated by Sepharose 4B gel filtration amounts to 37.000. Micaelis constants for glyoxylate and glutamate are corresponding to 6.25 X 10(-3) M and 2.75 X 10(-3) M, respectively. Optimal pH in phosphate and veronal buffers is 8.0 and optimal temperature 35--37 degrees C.
从植物乳杆菌提取物中分离得到了介导乙醛酸与谷氨酸反应生成甘氨酸和α -氧戊二酸的谷氨酸-乙醛酸转氨酶，并进行了84倍纯化。纯化后的酶需要磷酸吡哆醛和镁离子的加入才能发挥活性。Sepharose 4B凝胶过滤酶的分子量为37.000。glyoxylate和glutamate的Micaelis常数分别为6.25 X 10(-3) M和2.75 X 10(-3) M。磷酸盐和横向缓冲液的最佳pH值为8.0，最佳温度为35- 37℃。
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