Oral ethinylestradiol–levonorgestrel therapy counteracts fructose-induced renal metabolic impairment in female rats

Q3 Medicine Endocrine and Metabolic Science Pub Date : 2021-09-30 DOI:10.1016/j.endmts.2021.100099

Kehinde Samuel Olaniyi , Isaiah Woru Sabinari , Lawrence Aderemi Olatunji

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Abstract

Background: The excessive intake of fructose (FRD) induces insulin resistance (IR)-associated renal impairment. Similarly, the use of estrogen-progestin oral contraceptive therapy (EEL) has been associated with cardiometabolic syndrome, and its non-contraceptive benefits particularly in metabolic pathologies remain inconclusive. Therefore, the present study investigated the effects of EEL on renal metabolic function in rats exposed to FRD.

Methods: Female rats received vehicle (po), EEL (1.0 µg ethinylestradiol+5.0 µg levonorgestrel.), fructose (10%; w/v) and EEL+FRD respectively for 8 weeks. All data were expressed as means ± SEM and significance were accepted at p<0.05.

Results: Data revealed that FRD/EEL caused IR with correspondent increased plasma/renal lipid, decreased glucose-6-dehydrogenase (G6PD), glutathione (GSH) and renal NO/adenosine. FRD but not EEL increased (p<0.05) renal glycogen and decreased (p<0.05) plasma NO/adenosine and pancreatic beta-cell function. These alterations were attenuated when EEL was administered with FRD.

Conclusion: The study demonstrates that FRD causes renal impairments accompanied by deficient NO/adenosine concentration and defective G6PD/GSH-dependent antioxidant defense. The findings also suggest that EEL blots the renal effects of FRD.

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口服炔雌醇-左炔诺孕酮治疗可抵消果糖诱导的雌性大鼠肾脏代谢损伤

背景:过量摄入果糖(FRD)可诱导胰岛素抵抗(IR)相关的肾脏损害。同样，使用雌激素-黄体酮口服避孕药治疗(EEL)与心脏代谢综合征有关，其非避孕益处，特别是在代谢病理方面仍不确定。因此，本研究探讨了EEL对FRD暴露大鼠肾脏代谢功能的影响。方法:雌性大鼠给药(po)、EEL(1.0µg炔雌醇+5.0µg左炔诺孕酮)、果糖(10%;w/v)和EEL+FRD分别治疗8周。所有数据以均数±SEM表示，显著性以p<0.05接受。结果:数据显示，FRD/EEL引起IR，相应的血浆/肾脏脂质升高，葡萄糖-6-脱氢酶(G6PD)、谷胱甘肽(GSH)和肾脏NO/腺苷降低。FRD升高了肾糖原(p < 0.05)，降低了血浆NO/腺苷和胰岛β细胞功能(p < 0.05)。当与FRD一起给药时，这些改变减弱。结论:研究表明，FRD引起肾损害，并伴有NO/腺苷浓度不足和G6PD/ gsh依赖性抗氧化防御缺陷。研究结果还表明，EEL可以抑制FRD对肾脏的影响。

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