Influence of high hydrostatic pressure on the formation of keto and amino acids by a barotolerant strain of Pseudomonas aeruginosa.

Abstract

The peculiarities of the growth and extracellular accumulation of free keto and amino cids by a barotolerant culture (strain 0798) in culturing on Ran's glucose-mineral medium conditions of 1, 200, 300, and 500 atm were investigated. At a high pressure the culture possesses the ability for extracellular accumulation of free keto and amino acids, pyruvic, alpha-ketoglutaric, and oxaloacetic acids, alanine, and glutamic acid, while under conditions of atmospheric pressure, chiefly valine is accumulated in the medium, and there are no extracellular keto acids. The established specificity of the extracellular accumulation of keto and amino acids under conditions of high hydrostatic and atmospheric pressure is due to the different sensititivy of the enzyme systems participating in the biosynthesis of free keto and amino acids to the influence of high hydrostatic pressure.