Therapeutic Effect of Sinapic Acid against 5-Fluorouracil-Induced Oxidative Stress and Inflammation in Rat Ovarium: An Experimental Approach

Abstract

Tissue toxicity caused by 5-fluorouracil (5-FU) is associated with increased reactive oxygen species and inflammatory cytokines. Sinapic acid (SA) has both antioxidant and anti-inflammatory activities. Although SA has been shown to ameliorate chemical-induced tissue damage in various experimental models, its effects against 5-FU-induced ovarian damage have not yet been investigated. It was therefore aimed to evaluate the therapeutic potential of SA against 5-FU-induced ovarian damage in rats, together with the mechanisms of oxidative stress and inflammation for the first time in this study. Thirty rats were distributed into five groups: control, 5-FU (100 mg/kg) 5-FU+SA (2.5 and 5 mg/kg) and SA (5 mg/kg). 5-FU was applied to rats intraperitoneally on the 1st day of experiments and then SA was administrated for 3 successive days. Ovarian levels of lipid peroxidation [malondialdehyde (MDA)], oxidative stress (total oxidant status (TOS) and oxidative stress index (OSI)] antioxidant system [total antioxidant status (TAS), and catalase (CAT)], DNA damage [8-hydroxy-2'-deoxyguanosine (8-OHdG)] and inflammatory [interleukin-6 (IL-6)] markers in ovarian tissues were determined using spectrophotometric methods. It was determined that a single dose of 5-FU administration in rats significantly increased oxidative stress and inflammation in the ovarian tissue and suppressed the antioxidant system compared to the control group (p