{"title":"Labeled free Malachite Green immunosensor based on chitosan and gold nano particles","authors":"Min-Fu Wu, Min-hua Wu, Sha Li, X. Li","doi":"10.1109/ICEDME50972.2020.00119","DOIUrl":null,"url":null,"abstract":"An immunosensor for the detection of malachite green in water and fish samples was developed. GNPs with good biocompatibility and good electroconductivity were fabricated onto the surface of a gold electrode (GE). The polyclonal antibody was immobilized on the electrode. Under optimal conditions, the proposed immunosensor exhibited a sensitive response to malachite green in the range of 0.032 ~100 ng/mL, with a detection limit of 10.7 /mL in buffer. Recoveries of malachite green from spiked water and fish samples ranged from 84.2% to 113.8%, indicating that the proposed immunosensor potential can be used for the detection of real samples.","PeriodicalId":155375,"journal":{"name":"2020 3rd International Conference on Electron Device and Mechanical Engineering (ICEDME)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2020-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"2020 3rd International Conference on Electron Device and Mechanical Engineering (ICEDME)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/ICEDME50972.2020.00119","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
An immunosensor for the detection of malachite green in water and fish samples was developed. GNPs with good biocompatibility and good electroconductivity were fabricated onto the surface of a gold electrode (GE). The polyclonal antibody was immobilized on the electrode. Under optimal conditions, the proposed immunosensor exhibited a sensitive response to malachite green in the range of 0.032 ~100 ng/mL, with a detection limit of 10.7 /mL in buffer. Recoveries of malachite green from spiked water and fish samples ranged from 84.2% to 113.8%, indicating that the proposed immunosensor potential can be used for the detection of real samples.
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