Kinetic evaluation on cell growth and biosynthesis of polyhydroxybutyrate (PHB) by Bacillus safensis EBT1 from sugarcane bagasse

Q2 Engineering Engineering in Agriculture, Environment and Food Pub Date : 2018-07-01 DOI:10.1016/j.eaef.2018.03.003

Punniavan Sakthiselvan , Ramasamy Madhumathi

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引用次数: 18

Abstract

Polyhydroxybutyrate (PHB), a biodegradable polymer (an intracellular product) was produced using Bacillus safensis EBT1. A kinetic model that describes microbial growth, biopolymer production and substrate consumption was used to predict the performance of batch fermentation of Bacillus safensis EBT1. The experimental data were fitted with modified logistic equation. The specific growth rate (μ_max) and half saturation constant (K_s) values for the models such as Monod, Contois and Herbert were found to be 0.16, 0.15, 0.13 h⁻¹ and 79.51, 68.19, 43.39 g/L respectively. Leudeking-Piret kinetics indicated that PHB formation in this study as growth associated. The recovered polymer was characterized using Transmission Electron Microscopy, Differential Scanning Calorimetry, Thermogravimetry analysis and Nuclear Magnetic Resonance. The PHB produced was used to synthesize nanoparticles using modified nano-precipitation method.

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甘蔗渣中萨菲芽孢杆菌EBT1细胞生长及合成聚羟基丁酸酯(PHB)动力学评价

利用萨菲芽孢杆菌EBT1制备了一种可降解聚合物(胞内产物)聚羟基丁酸酯(PHB)。采用描述微生物生长、生物聚合物生产和底物消耗的动力学模型来预测萨芬芽孢杆菌EBT1分批发酵的性能。实验数据用修正logistic方程拟合。Monod、Contois和Herbert模型的比生长率μmax和半饱和常数Ks分别为0.16、0.15、0.13 h−1和79.51、68.19、43.39 g/L。Leudeking-Piret动力学表明PHB的形成与生长有关。利用透射电子显微镜、差示扫描量热法、热重法和核磁共振对所获聚合物进行了表征。将所得的PHB用改性纳米沉淀法合成纳米颗粒。

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来源期刊

Engineering in Agriculture, Environment and Food Engineering-Industrial and Manufacturing Engineering

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1.00

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期刊介绍： Engineering in Agriculture, Environment and Food (EAEF) is devoted to the advancement and dissemination of scientific and technical knowledge concerning agricultural machinery, tillage, terramechanics, precision farming, agricultural instrumentation, sensors, bio-robotics, systems automation, processing of agricultural products and foods, quality evaluation and food safety, waste treatment and management, environmental control, energy utilization agricultural systems engineering, bio-informatics, computer simulation, computational mechanics, farm work systems and mechanized cropping. It is an international English E-journal published and distributed by the Asian Agricultural and Biological Engineering Association (AABEA). Authors should submit the manuscript file written by MS Word through a web site. The manuscript must be approved by the author''s organization prior to submission if required. Contact the societies which you belong to, if you have any question on manuscript submission or on the Journal EAEF.