Expression Analysis of miR393 and Target Gene Ve of Solanum torvum Swartz Infected by Verticillium dahliae Kleb

Abstract

In this study, Solanum torvum Swartz. was used as material and B-Actin gene and U6 were used as internal references. First, the expression characteristics analysis of miR393 and target gene Ve were performed on the root, stem, leaf, flower and fruit of Solanum torvum Swartz. during flowering and fruiting stage. The strong Verticillium dahliae Kleb. were inoculated into Solanum torvum Swartz. during 5-6 leaf stage, and the control group was treated with ultrapure water. The Solanum torvum Swartz. roots were taken at 0 h, 3 h, 6 h, 12 h, 24 h, 36 h, 48 h and 72 h for expression analysis of infection by Verticillium dahliae Kleb. . The results showed that under normal growth conditions, miR393 and target gene Ve had specific expression patterns in different tissues of Solanum torvum Swartz., and the differential expression was significant. Both miR393 and target gene Ve had the highest expression levels in roots, followed by stems, and had the lowest levels in fruits. After infection by Verticillium dahliae Kleb., both miR393 and target gene Ve had certain expression in different infection periods. The expression of target gene Ve showed an upward tendency with the extension of infection time, and the highest expression level appeared at 48 h, followed by 72 h. While the expression of miR393 showed a downward tendency with the extension of infection time, and the highest expression level appeared at 6 h. The expression of miR393 and target gene Ve showed opposite tendencies in different infection periods. These results provided a foundation for further research on the interaction mechanism between miR393 and target gene Ve and the molecular mechanism of resistance to Verticillium wilt.