Identification of Aquatic Macro-invertebrates and Determination of E. coli and Heavy Metals in Rawal Lake, Pakistan

M. Ayub, G. Joshua, S. Nadeem, S. Awan, Z. B. Mirza, Anis-ur-Rahman
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Abstract

Water is an important constituent of life’s support system. However, industrial growth, urbanization and anthropogenic activities have affected the quality of water bodies mostly in the world. The aim of the study was to assess the water quality of Rawal Lake, Pakistan, which is source of drinking water for the residents of Islamabad and Rawalpindi, using macro-invertebrates as pollution indicators, and through microbial and heavy metal analysis. For this purpose, macro-invertebrates and water samples were collected from Rawal Lake (Korang Nala Entrance), Rawal Lake (Centre) and from Rawal Lake (Spillway) points. The collected samples were preserved and transported to the Kinnaird College laboratory, where macro-invertebrates like caddisfly, water boatman, water bugs, gilled snails, mayflies etc. were identified with the help of identification guides and keys, while the presence of E. coli and heavy metals: Cadmium, Copper, Chromium, Cobalt, Nickel, Manganese and Zinc in the water samples was assessed. The results showed that pollution sensitive macro-invertebrates were mostly found in Rawal Lake (Centre), indicating that the water undergoes a self-cleaning process, whereas, water samples of Rawal Lake (Korang Nala Entrance), Rawal Lake (Spillway) had mostly pollution tolerant species, which signified that the water of these areas was moderately polluted. Simpsons’ Index of Diversity for Rawal Lake indicated a high level of diversity in the area (0.85). Microbial analysis indicated the presence of E. coli in all the three sampling points of the lake. The levels of Cadmium (Korang Nala 0.007 ppm, Spillway 0.014 ppm) were found toexceed the World Health Organization permissible limits (0.005 ppm), in the water samples, however, the concentration values of other metals were within the permissible limits. Thus, it was concluded that the water of Rawal Lake was considered fit for drinking and other purposes in residential and commercial areas after being processed through a treatment plant.
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巴基斯坦拉瓦尔湖水生大型无脊椎动物的鉴定及大肠杆菌和重金属的测定
水是生命维持系统的重要组成部分。然而,在世界范围内,工业增长、城市化和人为活动对水体质量的影响最大。该研究的目的是利用大型无脊椎动物作为污染指标,并通过微生物和重金属分析,评估巴基斯坦拉瓦尔湖的水质。拉瓦尔湖是伊斯兰堡和拉瓦尔品第居民的饮用水来源。为此,从拉瓦尔湖(Korang Nala入口)、拉瓦尔湖(中心)和拉瓦尔湖(溢洪道)各点采集了大型无脊椎动物和水样。收集到的样品被保存并运送到Kinnaird学院实验室,在那里使用鉴定指南和钥匙鉴定了大型无脊椎动物,如caddisfly, water boatman, water bugs,鳃螺,mayflies等,同时评估了大肠杆菌和重金属:镉,铜,铬,钴,镍,锰和锌在水样中的存在。结果表明:罗瓦尔湖(中部)以污染敏感型大型无脊椎动物居多,表明水体经历了一个自清洁过程;而罗瓦尔湖(Korang Nala入口)、罗瓦尔湖(溢洪道)水样以耐污染型物种居多,表明这些地区的水体受到中度污染。拉瓦尔湖的simpson多样性指数(0.85)表明该地区生物多样性水平较高。微生物分析表明,三个采样点均存在大肠杆菌。在水样中发现镉的含量(Korang Nala为0.007 ppm, Spillway为0.014 ppm)超过了世界卫生组织允许的限值(0.005 ppm),但其他金属的浓度值在允许的范围内。因此,得出的结论是,Rawal湖的水经过处理厂的处理后,被认为适合住宅和商业区的饮用和其他用途。
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