Mycobacterium tuberculosis

Case Studies in Infectious Disease Pub Date : 1900-01-01 DOI:10.4324/9780203856871-23

P. Lydyard, Michael F. Cole, J. Holton, W. Irving, N. Porakishvili, P. Venkatesan, K. N. Ward

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Abstract

(TB) Mycobacterium tuberculosis (Mtb) that still constitutes a public health problem in Paraguay. This cross-sectional, descriptive, and observational pilot study aimed to characterize Mtbstrains isolated by the molecular technique “MycobacterialIn terspersedRepetitiveUnits-Variable NumberTandemRepeats” (MIRU-VNTR). It has been used 19 isolatedstrainscollected during 2008 at Hospital Km 81, Department of Cordillera. The samples have been analyzed in the Department of Molecular Biology and Biotechnology of the Research Institute in Health Sciences (hereinafter IICS, by its acronym in Spanish) of the National University of Asuncion where they were subjected to amplification of 12 MIRU-VNTR loci, followed by electrophoresis of polyacrylamide gel. Genetic patterns were classified using the MIRU-VNTRplus bioinformatics tool. The Hunter Gaston Diversity Index (HGDI) determined that the MIRU 10 and MIRU 40 loci had the highest discriminatory power. Four strains were found in 2 clades with 100% similarity. In addition, 12/19 strains were found in 4 clades with 80% similarity. Additional studies are necessary to determine epidemiological associations. MIRU-VNTR grouped the strains studied with members of the LAM, Haarlem and X families with a predominance of the LAM family (79%).

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结核分枝杆菌

(TB)结核分枝杆菌(Mtb)仍然是巴拉圭的一个公共卫生问题。这项横断面、描述性和观察性的初步研究旨在描述通过分子技术“MycobacterialIn terspersedrepetiveunits - variable NumberTandemRepeats”(MIRU-VNTR)分离的mt菌株。采用2008年在科迪勒拉县81号医院采集的19株分离菌株。这些样品在亚松森国立大学健康科学研究所(以下简称IICS，其西班牙语首字母缩写)的分子生物学和生物技术系进行了分析，并对12个MIRU-VNTR位点进行了扩增，然后进行聚丙烯酰胺凝胶电泳。使用MIRU-VNTRplus生物信息学工具对遗传模式进行分类。亨特加斯顿多样性指数(HGDI)确定MIRU 10和MIRU 40位点具有最高的歧视能力。在2个进化支中发现4株，相似性为100%。在4个支系中发现了12/19株相似度为80%的菌株。需要进一步的研究来确定流行病学关联。MIRU-VNTR将所研究的菌株分为LAM、Haarlem和X家族，其中LAM家族占优势(79%)。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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