Preparation and Characterization of Microbial Growth Media and its Application for the Production of Microbial Feed Protein

Abstract

Cereal and grains have been using as a source of food molecules for centuries. However, the preparation of microbial growth media from a particular cereal and grain for the growth of microorganisms has not been considered. In this study, preparation of microbial growth media for the cultivation of Bacillus subtilis strain st-103 was done using rye pellets. The rye pellets, obtained after the hydrolysis of rye grains were enriched using Bacillus subtilis strain st-103 producer of protein derived by the ecological selection.The cultivation process of Bacillus subtilis strain st-103 was carried out under aerobic conditions in an incubator shaker (ES-20) at 28 ℃ at 250 rpm. The total viable microorganisms are grown on the media were determined according to standard test method for automated colony forming unit (CFU) assay. According to the result of the experiment, the total viable count of Bacillus subtilis strain st-103 was 1.2 X 108 CFU/ml. Based on the result obtained in the course of scientific work suggest the prospects for further steps of the enrichment of rye grain ( pellets) with Bacillus subtilis strain st-103 to produce microbial feed protein.