Influence of amino acids on the activity of cellulolytic enzymes obtained from spent medium of Fusarium cultures and on the "Cellulase S" Novo.

C Szajer
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Abstract

Comparative studies on the influence of 4 amino acids (cysteine, cystine, methionine and alanine) on the activity of enzymatic preparation were carried out. The action of the amino acids was shown to depend on pH, incubation time and the temperature of incubation. Attempts were made to find a correlation between the amino acids introduced and redox potential (Eh), pH and the activity of the cellulolytic enzymes. It was found that the addition of some amino acids to the reaction mixtures at different pH values affected the value of Eh -- which enhanced the cellulase activity.

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氨基酸对镰刀菌废培养基纤维素酶活性的影响及对“纤维素酶S”Novo的影响。
比较研究了半胱氨酸、胱氨酸、蛋氨酸和丙氨酸4种氨基酸对酶促制剂活性的影响。氨基酸的作用取决于pH值、孵育时间和孵育温度。试图找出氨基酸引入与氧化还原电位(Eh)、pH和纤维素水解酶活性之间的关系。研究发现,在不同pH值的反应混合物中加入一些氨基酸会影响Eh值,从而提高纤维素酶的活性。
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