Inhibition Test of Hot Water Extract of Rhizophora apiculata Leaves Against Edwardsiella tarda Bacteria in-Vitro

Jurnal Natur Indonesia Pub Date : 2022-04-12 DOI:10.31258/jnat.20.1.30-34

R. Kurniawan, S. Wahyuni, Fitrian Armando

引用次数: 1

Abstract

One of the plants that have the potential as antibacterial ingredients is Rhizophora apiculata leaf because it contains secondary metabolites, such as tannins, saponins, flavonoids, steroids, and terpenoids. This study aims to find out the inhibition of hot water extract of R. apiculata leaves in inhibiting the growth of Edwardsiella tarda. The research method used is an experimental method which includes sample preparation, extraction, antibacterial activity test using the Kirby-Bauer disc method and applying a Completely Randomized Design (CRD). The doses used were 100% (10000 ppm), 90% (9000 ppm), 80% (8000 ppm), 70% (7000 ppm), 60% (6000 ppm), 50% (5000 ppm), 40% (4000 ppm), 30% (3000 ppm), 20% (2000 ppm), 10% (1000 ppm) and control (Oxytetracycline). The results showed that the hot water extract of R. apiculata leaves at a dose of 1000-10000 ppm gave various inhibitory diameters ranging from 6.25-10.08 mm and the clear zone formed was classified as medium

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尖根参叶热水浸提液对迟缓爱德华菌体外抑制试验

其中一种具有潜在抗菌成分的植物是尖根草叶子，因为它含有次级代谢物，如单宁、皂苷、黄酮类化合物、类固醇和萜类化合物。本研究旨在研究荆芥叶热水提取物对迟缓爱德华菌生长的抑制作用。研究方法采用实验方法，包括样品制备、提取、Kirby-Bauer圆盘法抗菌活性测试，采用完全随机设计(CRD)。使用的剂量分别为100% (10000 ppm)、90% (9000 ppm)、80% (8000 ppm)、70% (7000 ppm)、60% (6000 ppm)、50% (5000 ppm)、40% (4000 ppm)、30% (3000 ppm)、20% (2000 ppm)、10% (1000 ppm)和对照(土霉素)。结果表明，在1000 ~ 10000 ppm的剂量下，尖叶参叶热水提取物的抑菌直径在6.25 ~ 10.08 mm之间，形成的透明区为中等

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Jurnal Natur Indonesia

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