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Reduction of Dispersant Pollutant (Linear Alkylbenzene Sulfonate) by Mangrove Plant (Rhizophora mucronata) 红树植物(Rhizophora mucronata)对分散剂污染物(线性烷基苯磺酸盐)的还原作用
Pub Date : 2023-11-13 DOI: 10.31258/jnat.21.2.162-166
S. Nedi, Irwan Effendi, A. Tanjung, E. Elizal
Linear alkylbenzene sulfonate (LAS) is the main component of dispersants that function as cleaners of various materials. The increasing use of LAS causes this compound to dominantly pollute coastal waters.  Phytoremediation is the most environmentally safe alternative to physical and chemical methods. One of the potential plants as a phytoremediator is Rhyzophora mucronata. This research is an experiment as an application of LAS pollutant control by Rhyzophora Mucronata (R. Mucronata) plants in waters.  After acclimatization and preliminary tests, the implementation of LAS phytoremediation tests by R. Mucronata plants in seawater media was carried out at concentrations of A1: 25 mg/L, A2: 100 mg/L, A3: 175 mg/L, A4: 250 mg/L, and A5: 325 mg/L and control (Control without plants) as a simulation of the presence of pollutants in coastal waters.   The method used was a completely randomized design (CRD) with one treatment and 3 replications. The reduction of LAS concentration in the test media and its effect on R Mucronata leaf chlorophyll and dissolved oxygen were analyzed at week 2 to week 4. The analysis results showed that LAS compounds could be reduced to 91.48% at week 2 and 97.40% at week 4. Plant Water quality parameters (dissolved oxygen, temperature and pH) were measured daily. Based on the study results, LAS can be reduced by R. Mucronata plants 87-90.4% in week 2 and 95.1-97.4% in week 4. LAS exclusion by plants had a significant impact on the reduction of leaf chlorophyll content until week 4. LAS reduction by R. Mucronata in the media can increase dissolved oxygen content as an indication of improved water quality. R. Mucronata plants can be used as an alternative to control LAS pollution in waters
线性烷基苯磺酸盐(LAS)是用作各种材料清洁剂的分散剂的主要成分。线性烷基苯磺酸盐(LAS)的使用量不断增加,导致这种化合物对沿海水域造成了严重污染。 植物修复是物理和化学方法之外对环境最安全的替代方法。Rhyzophora mucronata 是一种潜在的植物修复剂。本研究是一项在水域中应用 Rhyzophora Mucronata(R. Mucronata)植物控制 LAS 污染物的实验。 经过适应性测试和初步测试后,在海水介质中进行了褐藻酸植物修复试验,浓度分别为 A1:25 毫克/升、A2:100 毫克/升、A3:175 毫克/升、A4:250 毫克/升、A5:325 毫克/升和对照(无植物对照),以模拟沿海水域污染物的存在。 采用的方法是完全随机设计(CRD),一个处理 3 次重复。在第 2 周至第 4 周分析了试验介质中 LAS 浓度的降低及其对 R Mucronata 叶片叶绿素和溶解氧的影响。分析结果表明,在第 2 周和第 4 周,LAS 化合物的浓度分别降低了 91.48% 和 97.40%。每天测量植物水质参数(溶解氧、温度和 pH 值)。研究结果表明,在第 2 周,R. Mucronata 植物可减少 87-90.4% 的 LAS,在第 4 周可减少 95.1-97.4% 的 LAS。植物排除 LAS 对叶片叶绿素含量的降低有显著影响,直到第 4 周。介质中的 R. Mucronata 能减少 LAS,从而增加溶解氧含量,这表明水质得到了改善。R. Mucronata 植物可用作控制水体中 LAS 污染的替代品
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引用次数: 0
The Addition of Guava Leaves in Feed to the Blood Glucose of Carp reared in Brackish Water and Infected with Aeromonas hydrophila 在饲料中添加番石榴叶对在咸水中饲养并感染嗜水气单胞菌的鲤鱼血糖的影响
Pub Date : 2023-11-13 DOI: 10.31258/jnat.21.2.156-161
Irwan Effendi, W. Windarti, Heri Masjudi, M. Razman, Ahmed Al-Harbi, Nisfi Maulidia Nasution, Tomi Syahputra, R. Kurniawan
Guava leaves can be utilized as feed additives and medicine for aquaculture. Limiting factors in aquaculture, including stocking density, and salinity can cause stress in fish, so they are easily infected with disease. This study was conducted to determine the effect of guava on the blood glucose of carp reared in brackish water media. This research was conducted from August to September 2023 at the Marine Biotechnology Laboratory, Faculty of Fisheries and Marine, Universitas Riau. The method used in this study is an experimental method using a completely randomized design with 5 (five) treatments and 3 (three) replicates, the treatments are T0 (control), T1 (1.0g), T2 (1.5g), T3 (2 g), and T4 (2.5g). Carp measuring 5.00 ± 1.00 cm with a weight of 4.00 ± 0.50 g were obtained from farmers in the Koto Panjang hydroelectric reservoir, Sumatra, Indonesia. Fish rearing was carried out for 60 days. The results showed that the provision of guava leaves in the feed affected the blood glucose levels of carp. The dose of 1.5 g/100g feed (T2) was the optimal dose in increasing the blood glucose of carp reared in brackish water media and infected with A. hydrophila bacteria
番石榴叶可用作水产养殖的饲料添加剂和药物。水产养殖中的限制性因素,包括放养密度和盐度,会导致鱼类产生应激反应,因此很容易感染疾病。本研究旨在确定番石榴对在咸水介质中饲养的鲤鱼血糖的影响。这项研究于 2023 年 8 月至 9 月在廖内大学渔业与海洋学院海洋生物技术实验室进行。本研究采用的方法是完全随机设计的实验法,有 5 个处理和 3 个重复,处理为 T0(对照)、T1(1.0 克)、T2(1.5 克)、T3(2 克)和 T4(2.5 克)。鲤鱼体长 5.00 ± 1.00 厘米,体重 4.00 ± 0.50 克,来自印度尼西亚苏门答腊岛 Koto Panjang 水电站水库的养殖户。鱼类饲养期为 60 天。结果表明,在饲料中添加番石榴叶会影响鲤鱼的血糖水平。每 100 克饲料中添加 1.5 克番石榴叶的剂量(T2)是提高在咸水培养基中饲养的鲤鱼血糖的最佳剂量。
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引用次数: 0
Diversity and Abundance of Epiphytic Diatoms on Seagrass Leaves (Enhalus acoroides) in Poncan Gadang Island Sibolga City, North Sumatra 北苏门答腊西博尔加市庞坎加当岛海草叶片(Enhalus acoroides)上附生硅藻的多样性和丰度
Pub Date : 2023-11-01 DOI: 10.31258/jnat.21.2.149-155
Dinda Trie K Hayati, S. H. Siregar, R. Rifardi
The seagrass ecosystem is one of the marine ecosystems that has an important role, namely as a source of life for organisms in the sea. Diatoms are microalgae widely distributed throughout the aquatic environment and are even found in plants, including seagrass leaves. Diatoms greatly influence life in the waters because they play an essential role as a food source for various marine organisms and play a role in the transfer of carbon, nitrogen, and phosphate. This research was conducted in February 2023 on Poncan Gadang Island, Sibolga City, North Sumatra. The study aims to determine the density, relative abundance, abundance, and diversity of epiphytic diatom species on seagrass (Enhalus acoroides) leaves in Poncan Gadang Island. The method used is the survey method, using three stations to take samples. The determination of 3 stations is based on the density of the seagrass beds ecosystem. Sampling of diatoms was carried out by brushing the surface of seagrass leaves about 5x2 cm on the front and back of the leaves. The samples obtained were then analyzed in the laboratory. There were 12 types of diatoms found, consisting of Cocconeis sp., Rhizosolenia sp., Biddulphia sp., Amphipluera sp., Thalassiotrix sp., Nitzchia sp., Synedra sp., Melosira sp., Pleurogosigma sp., Skeletonema sp., Licmiphora sp., and Thalassionema sp. the average value of epiphytic diatom abundance in seagrass leaves ranged from 2160.85 – 4649.94 ind/cm². The epiphytic diatom diversity (H') values ranged from 2.53 to 2.72
海草生态系统是海洋生态系统之一,具有重要作用,即作为海洋生物的生命之源。硅藻是一种微藻类,广泛分布于水生环境中,甚至在植物(包括海草叶片)中也能发现。硅藻对水域中的生物有很大影响,因为它们是各种海洋生物的重要食物来源,并在碳、氮和磷酸盐的转移过程中发挥着重要作用。这项研究于 2023 年 2 月在北苏门答腊锡伯加市的庞坎加当岛上进行。该研究旨在确定 Poncan Gadang 岛海草(Enhalus acoroides)叶片上附生硅藻物种的密度、相对丰度、数量和多样性。采用的方法是调查法,在三个站点取样。确定三个站点的依据是海草床生态系统的密度。硅藻采样是通过刷洗海草叶片正面和背面约 5x2 厘米的表面进行的。获得的样本随后在实验室进行分析。共发现 12 种硅藻,包括 Cocconeis sp.、Rhizosolenia sp.、Biddulphia sp.、Amphipluera sp.、Thalassiotrix sp.、Nitzchia sp.、Synedra sp.、Melosira sp、海草叶片中附生硅藻丰度的平均值介于 2160.85 - 4649.94 ind/cm² 之间。附生硅藻多样性(H')值介于 2.53 和 2.72 之间。
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引用次数: 0
Stomatal Characteristics in Three Groups of Mangrove Plants: Major, Minor, and Associate 三类红树植物的气孔特征:主要红树植物、次要红树植物和辅助红树植物
Pub Date : 2023-10-30 DOI: 10.31258/jnat.21.2.144-148
Febrina Artauli Siahaan, Dewi Lestari
Three types of mangroves inhabit different environmental gradients and zones within the mangrove ecosystem. These three mangrove types exhibit distinct adaptation strategies. Stomata, as a crucial organ of the plant system, play a significant role in the adaptability of plants. Understanding stomata is essential for comprehending the physiological conditions and responses of plants to environmental conditions. This research aimed to study the stomatal characteristics of three different mangrove types and gain a deeper understanding of their potential adaptive strategies. This research was conducted at the Mangrove Botanical Garden Gunung Anyar, Surabaya, in November 2022. We selected two representative mangrove species from the major, associate, and minor types. Stomata of each mangrove species were observed to identify the stomatal type, density, and size using an Olympus microscope at 100 xs and 400 x magnifications. The results showed there were variations in stomatal type, density, and stomatal length and width among studied mangrove types. Major mangrove species exhibited the lowest stomatal density and the largest size of stomata compared to minor and associated mangroves. The diversity of stomatal traits among major, associate, and minor mangrove types underscores their adaptations and ecological strategies.
在红树林生态系统中,有三种类型的红树林栖息在不同的环境梯度和区域。这三种类型的红树林表现出不同的适应策略。气孔作为植物系统的重要器官,在植物的适应性方面发挥着重要作用。了解气孔对于理解植物的生理状况和对环境条件的反应至关重要。本研究旨在研究三种不同类型红树林的气孔特征,深入了解其潜在的适应策略。这项研究于 2022 年 11 月在泗水古农安亚尔红树林植物园进行。我们从主要类型、副类型和次要类型中选择了两个具有代表性的红树林物种。使用奥林巴斯显微镜在 100 xs 和 400 x 放大倍率下观察每个红树林物种的气孔,以确定气孔类型、密度和大小。结果表明,所研究的红树林类型在气孔类型、密度、气孔长度和宽度方面存在差异。与次红树林和伴生红树林相比,主要红树林物种的气孔密度最低,气孔尺寸最大。主要红树林、伴生红树林和次要红树林类型气孔特征的多样性凸显了它们的适应性和生态策略。
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引用次数: 0
Inventory of Large Mammals in Ujung Kulon National Park, Banten Province 万丹省乌戎库隆国家公园大型哺乳动物清单
Pub Date : 2023-10-30 DOI: 10.31258/jnat.21.2.134-143
Zulia Pratiwi, Diva Novi Sandrian, Ayu Octavia, Nisa Luthfiah, Riska Rahmawati, Silvia Riskina Said, Neneng Nuraliah
The National Park has a diverse ecosystem for the preservation of flora and fauna, making Ujung Kulon National Park a habitat for endemic and protected fauna. Large mammals are one of the many animals found in TNUK, especially those with protected status. This study aims to determine the diversity of large mammal species in Ujung Kulon National Park. The research was conducted on September 27–29, 2022, in Ujung Kulon National Park, which is located at the western tip of Java Island, precisely in Sumur and Cimanggu Districts, Pandeglang Regency, Banten Province. The method used was the cruising method using exploratory descriptive data analysis. The results showed that there were two types of large mammals, namely ungulate mammals and primate mammals. The number obtained was 10 types of mammal species belonging to 7 different families. Large mammals found include Rhinoceros sondaicus, Muntiacus muntjak, Cervus timorensis, Hyolobates moloch, Presbytis comata, Trachypitecus auratus, Macaca fascicularis, Sus scrofa, Tragulus javanicus, and Bubalus bubalis. Most of the large mammals found are endemic mammals with protected status. Of the large mammals found, the most common were hoofed mammals. With this study, the public has an awareness of the importance of maintaining the diversity of fauna and flora in Ujung Kulon National Park
国家公园拥有保护动植物的多样化生态系统,使乌戎库隆国家公园成为特有动物和受保护动物的栖息地。大型哺乳动物是乌戎库隆国家公园发现的众多动物之一,尤其是那些受保护的动物。本研究旨在确定乌戎库隆国家公园大型哺乳动物物种的多样性。研究于 2022 年 9 月 27-29 日在乌戎库隆国家公园进行,该国家公园位于爪哇岛西端,准确地说是万丹省潘德格朗县苏木尔区和西芒古区。采用的方法是探索性描述数据分析的巡游法。结果显示,大型哺乳动物有两类,即有蹄类哺乳动物和灵长类哺乳动物。获得的哺乳动物种类有 10 种,隶属于 7 个不同的科。发现的大型哺乳动物包括犀牛(Rhinoceros sondaicus)、驼鹿(Muntiacus muntjak)、马鹿(Cervus timorensis)、驼鸟(Hyolobates moloch)、驼鸟(Presbytis comata)、驼鸟(Trachypitecus auratus)、猕猴(Macaca fascicularis)、苏门羚(Sus scrofa)、獐子(Tragulus javanicus)和小鹿(Bubalus bubalis)。发现的大多数大型哺乳动物都是受保护的特有哺乳动物。在发现的大型哺乳动物中,最常见的是有蹄类哺乳动物。通过这项研究,公众认识到了维护乌戎库隆国家公园动植物多样性的重要性。
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引用次数: 0
Study of Gastropod Community Structure After Development of Tourism Activities on the Senjoyo River 森乔约河旅游活动开发后的腹足类群落结构研究
Pub Date : 2023-10-30 DOI: 10.31258/jnat.21.2.124-133
Marsahanda Astri Ramagita, Sucahyo Sucahyo, Desti Christian Cahyaningrum
It is thought that the development of the Senjoyo River as a tourist attraction in Semarang Regency will cause changes to the natural ecosystem in the area, one of which is changing the structure of the existing gastropod community. This research is a quantitative analysis research using a survey method that aims to examine the structure of the gastropod community after the development of tourism activities on the Senjoyo River. Gastropod samples were taken from 10 different observation stations along the Senjoyo River, which are located at 7˚22'23"S and 110˚31'37"E. Observation station locations were determined by the purposive random sampling method, based on tourism activity density. The result showed types of gastropods that were found in the Senjoyo River namely, Sulcospira testudinaria, Tarebia granifera, Brotia costula, Thiara scraba, and Melanoides tuberculata with clustered distribution patterns (Id>1). Statistical analysis showed that location affected gastropod abundance (Asymp. Sig of 0.043), with the highest abundance found at station seven (45,11 individuals/m2). So station seven, with sandy and muddy substrate conditions, moderate current speed (0.257 m/s), and rarely found human activity, is the most suitable location to support the survival of gastropods in the Senjoyo River. Furthermore, the gastropod community structure indicates environmental changes at observation stations nine and ten, the locations where the most activities and construction of tourism facilities were found. That was indicated by the low diversity (H’= 0.00-0.01) and evenness (E = 0.00) index of gastropods, and there is dominance by Sulcospira testudinaria (C= 0.98-1.00).
人们认为,将仙乐瑶河开发为三宝垄地区的旅游景点将导致该地区的自然生态系统发生变化,其中之一就是改变现有腹足类动物群落的结构。本研究是一项采用调查方法的定量分析研究,旨在考察仙乐悠河旅游活动开发后腹足类动物群落的结构。研究人员在位于南纬7˚22'23 "和东经110˚31'37 "的森乔沃河沿岸的10个不同观察站采集了腹足类样本。结果显示,在森乔约河发现的腹足类动物种类有:Sulcospira testudinaria、Tarebia granifera、Brotia costula、Thiara scraba 和 Melanoides tuberculata,它们呈群集分布模式(同上>1)。统计分析显示,地点影响腹足类动物的丰度(Asymp.因此,底质为沙质和泥质、水流速度适中(0.257 米/秒)、人类活动稀少的七号站是森乔约河中最适合腹足类生存的地点。此外,腹足类群落结构表明第九和第十观测站的环境发生了变化,这两个观测站是活动和 旅游设施建设最多的地方。这表现在腹足类生物的多样性(H'= 0.00-0.01)和均匀度(E=0.00)指数较低,而且 Sulcospira testudinaria(C= 0.98-1.00)占优势。
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引用次数: 0
Pancreas Histology of Hyperglycemia of Mice (Mus musculus L.) After Treatment Basil (Ocimum x africanum Lour.) Leaf Extract and Papaya (Carica papaya L.) 罗勒(Ocimum x africanum Lour.)叶提取物和木瓜(Carica papaya L.)处理高血糖小鼠(Mus musculus L.)后的胰腺组织学研究
Pub Date : 2023-10-26 DOI: 10.31258/jnat.21.2.118-123
Elsa Safitri, Sutyarso Sutyarso, Sri Wahyuningsih, R. Agustrina
Hyperglycemia is a condition in which the level of glucose in the blood is abnormally high. Hyperglycemia is caused by disturbances in the metabolic system in secreting insulin. Hyperglycemia if left unchecked can cause a disease which is usually called diabetes. Basil (Ocimum x africanum Lour.) and papaya (Carica papaya L.) leaf extracts can reduce blood glucose levels in people with diabetes mellitus because they contain active compounds of flavonoids, alkaloids and tannins which function as anti-diabetics. The aim of this study was to examine the effectiveness of the effects of basil and papaya leaf in affecting the level of islet damage, and repairing pancreatic histological damage in hyperglycemic mice. This study used a completely randomized design with 5 treatment groups and 5 replications. Group K(N) as normal control, group K(+) as positive control, group K (-) as negative control, group P 1 (alloxan induced and treated with ethanol extract of basil leaves 24.5 mg/35g BW mice) 14 days oral) , P 2 group (induced by alloxan and treated with papaya leaf ethanol extract 24.5 mg/35 g BW mice (14 days oral). Data on the level of histological damage to the pancreas of hyperglycemic mice were analyzed using the Kruskal-Wallis method and the Wilcoxon-MannWhitney test at a significant level of 5%. Data histological appearance of the Langerhans were analyzed descriptively.The results showed basil leaf extract and papaya had an effect on the level of damage to the pancreas of mice, basil leaf extract was effective compared to papaya in histological repair of the mice pancreas
高血糖症是指血液中葡萄糖水平异常升高。高血糖是由于新陈代谢系统分泌胰岛素的功能紊乱引起的。如果不加以控制,高血糖会导致疾病,通常称为糖尿病。罗勒(Ocimum x africanum Lour.)和木瓜(Carica papaya L.)叶提取物可以降低糖尿病患者的血糖水平,因为它们含有黄酮类、生物碱和单宁酸等活性化合物,具有抗糖尿病的作用。本研究的目的是检测罗勒和木瓜叶在影响高血糖小鼠胰岛损伤程度和修复胰腺组织损伤方面的效果。本研究采用完全随机设计,设 5 个治疗组,5 次重复。K(N)组为正常对照组,K(+)组为阳性对照组,K(-)组为阴性对照组,P 1 组(阿脲诱导并用罗勒叶乙醇提取物 24.5 毫克/35 克体重小鼠治疗,口服 14 天),P 2 组(阿脲诱导并用木瓜叶乙醇提取物 24.5 毫克/35 克体重小鼠治疗,口服 14 天)。采用 Kruskal-Wallis 法和 Wilcoxon-MannWhitney 检验法对高血糖小鼠胰腺组织学损伤程度的数据进行分析,显著水平为 5%。结果表明,罗勒叶提取物和木瓜对小鼠胰腺的损伤程度有影响,罗勒叶提取物对小鼠胰腺组织学修复的效果优于木瓜。
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引用次数: 0
Optimalization of Water for Nursery and Rearing of Asian Redtail Catfish (Mystus nemurus C.V) 优化亚洲红尾鲶鱼(Mystus nemurus C.V)的育苗和饲养水质
Pub Date : 2023-10-24 DOI: 10.31258/jnat.21.2.109-117
Mulyadi Mulyadi, N. A. Pamukas
Fish culture activities usually release wastes to environments such as faeces, urine, uneaten food and other by-product metabolism activities. For increased water quality, ideally in aquaculture media, various methods could be used, such as filtering techniques and sinking and dissolving methods. Various filter materials could be used to increase water quality, such as silt, gravel, charcoal, coconut shell, palm fiber, and zeoliteThe study on the effect of recirculation systems using various filter materials and aquaponic systems on water quality has been carried out. Several filter materials were used in four kinds of treatment,  namely aquarium using aerators (control), aquarium using silt, gravels and palm fiber (recirculation), aquarium using mustard greens (aquaponic system) and aquarium using spoons (recirculation). The study results showed that different filter materials significantly affected nitrate and nitrite concentration in the catfish culture media. It was invented that the treatment of a recirculation system combined with an aquaponic system could increase water quality parameters such as  CO2 (7.99 - 11.98 mg.L-1), NH3 (0.02 - 0.07 mg.L-1), NO2 (2.43 - 0.02 mg.L-1), NO3 (4.32 - 0.04 mg.L-1) respectively. Still, the other water quality parameters were similar. The best results were achieved at the same treatment, namely absolute growth rates  (4.01 g), daily growth rates  (1.96 %) and survival rates (91.11 %), respectively
鱼类养殖活动通常会向环境排放废物,如粪便、尿液、未吃完的食物和其他新陈代谢活动的副产品。为了提高水质,最好是在水产养殖媒介中,可以使用各种方法,如过滤技术、沉降和溶解方法。研究了使用各种过滤材料的循环系统和水产养殖系统对水质的影响。在四种处理方式中使用了几种过滤材料,即使用充气机的水族箱(对照)、使用淤泥、砾石和棕榈纤维的水族箱(再循环)、使用芥菜的水族箱(水生植物系统)和使用勺子的水族箱(再循环)。研究结果表明,不同的过滤材料对鲶鱼养殖介质中硝酸盐和亚硝酸盐的浓度有明显影响。研究发现,将循环系统与水产养殖系统结合使用可提高水质参数,如 CO2(7.99 - 11.98 mg.L-1)、NH3(0.02 - 0.07 mg.L-1)、NO2(2.43 - 0.02 mg.L-1)、NO3(4.32 - 0.04 mg.L-1)。其他水质参数仍然相似。在相同的处理条件下取得的效果最好,即绝对生长率(4.01 克)、日生长率(1.96 %)和存活率(91.11 %)分别为
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引用次数: 0
Bioremediation, Biosorption and Biodegradation of the Textile Dye Reactive Black 5 by Life Cultures of Trichoderma asperellum LBKURCC1 毛霉 LBKURCC1 生命培养物对纺织染料活性黑 5 的生物修复、生物吸附和生物降解作用
Pub Date : 2023-10-18 DOI: 10.31258/jnat.21.2.93-99
Titania Tjandrawati Nugroho, Putri Nabilah, Nuria Wulandari, Y. Nurulita, A. Dahliaty, Yanti Yanti
Reactive black 5 (RB5) is an azo dye widely used in the textile industry for dyeing fabrics. It is categorized as a recalcitrant dye that is hard to degrade and an environmental pollutant. Therefore, textile waste effluents containing this dye must be treated to remove or degrade the dye, before being released into the environment. One method that can be used to degrade synthetic dyes such as RB5 is to use biological methods, by directly using live fungal cells or using laccase enzymes. Trichoderma asperellum LBKURCC1 is a filamentous fungus isolated from cacao plantation soil in Riau, Indonesia, and it is a laccase enzyme producer. To be able to determine the ability of T. asperellum LBKURCC1 life cultures to decolorize RB5 dye, several RB5 dye removal tests were carried out. Incubation of 50 ppm RB5 with life cultures of T. asperellum LBKURCC1 at room temperature (30°C, pH 6.5) for 24 hours resulted in 22% bioremediation, 3.2% biosorption and 19.1% biodegradation of the RB5 dye.  The results of this study show that the live culture of T.asperellum LBKURCC1 is capable of biodegrading RB5. This is indicated by the degradation of RB5 by extracellular enzymes produced by these filamentous fungi.
活性黑 5(RB5)是一种偶氮染料,广泛用于纺织业的织物染色。它被归类为难降解染料和环境污染物。因此,含有这种染料的纺织废水必须经过处理,去除或降解染料后才能排放到环境中。降解 RB5 等合成染料的一种方法是使用生物方法,即直接使用活的真菌细胞或使用漆酶。毛霉 LBKURCC1 是一种从印度尼西亚廖内省可可种植园土壤中分离出来的丝状真菌,它是一种漆酶生产者。为了确定 T. asperellum LBKURCC1 生命培养物对 RB5 染料的脱色能力,我们进行了多项 RB5 染料去除试验。在室温(30°C,pH 6.5)下,将 50 ppm RB5 与 T. asperellum LBKURCC1 生命培养物培养 24 小时后,RB5 染料的生物修复率为 22%,生物吸附率为 3.2%,生物降解率为 19.1%。 研究结果表明,T.asperellum LBKURCC1 的活培养物能够生物降解 RB5。这些丝状真菌产生的胞外酶降解了 RB5。
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引用次数: 0
The Effect of Different Substrates on Laboratory Scale Cultivation of Kappaphycus alvarezii 不同基质对实验室规模培养阿尔瓦雷茨卡帕藻的影响
Pub Date : 2023-10-18 DOI: 10.31258/jnat.21.2.100-108
Muhammad Heri Hariadi, N. Cokrowati, Mukammad Marzuki
Seaweed is a leading commodity in the Indonesian fisheries sector. Indonesia is the second largest seaweed-producing country in the world after China. K.alvarezii is a type of seaweed that produces kappa carrageenan which can be used as a raw material in industry, making K.alvarezii seaweed very popular for cultivation. Cultivation of K.alvarezii seaweed has developed widely in Indonesian waters. The success of cultivating K.alvarezii seaweed can be achieved if it is supported by a suitable environment for its growth. One of the environmental aspects that influences the growth of K.alvarezii seaweed is the bottom substrate of the waters. This research aims to analyze the best substrate for the survival rate of brown K.alvarezii seaweed cultivated on a laboratory scale. This research used an experimental method using a Completely Randomized Design (CRD) consisting of 4 treatments and each treatment was carried out 3 times to obtain 12 experimental units. The treatments tested were different substrates consisting of sand, coral, volcanic rock, and coral sand. The results of the research show that different types of substrate have a real influence on the survival rate of brown K.alvarezii seaweed cultivated on a laboratory scale. Coral substrate gave the best results with a survival rate of 100%, final weight of 20 g, and carrageenan yield value of 26%.
海藻是印度尼西亚渔业部门的主要商品。印尼是仅次于中国的世界第二大海藻生产国。K.alvarezii 是一种能产生卡帕卡拉胶的海藻,这种卡拉胶可用作工业原料,因此 K.alvarezii 海藻的种植非常受欢迎。K.alvarezii 海藻的种植已在印度尼西亚海域广泛开展。如果有适合海藻生长的环境支持,就能成功培育 K.alvarezii 海藻。影响 K.alvarezii 海藻生长的环境因素之一是水域的底质。本研究旨在分析在实验室规模上培育的棕色 K.alvarezii 海藻存活率的最佳底质。本研究采用完全随机设计(CRD)的实验方法,包括 4 个处理,每个处理进行 3 次,共 12 个实验单元。测试的处理是由沙子、珊瑚、火山岩和珊瑚沙组成的不同基质。研究结果表明,不同类型的基质对实验室规模培育的棕色 K.alvarezii 海藻的存活率有实际影响。珊瑚基质的效果最好,存活率为 100%,最终重量为 20 克,卡拉胶产量值为 26%。
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Jurnal Natur Indonesia
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