RAPD analysis on genetic diversity of typical tea populations in Hunan Province

Abstract

Genetic diversity and genetic variation of 240 adult plants of four tea populations,Camellia sinensis var.sinensis,C.sinensis var.assamica cv.Duntsa,C.ptilophylla and C.sinensis var.assamica cv.Jianghua in Hunan,were studied by using RAPD markers.The results showed that 226 loci were found by using 21 random primers(10 bp),of which 201 loci were polymorphic(88.9%).The analyzing result of genetic diversity indicated that Shannon's index was 0.43,74.7% of which was within population genetic diversity while 25.3% was among population variation;the gene diversity of total populations(HT)of Nei's index was 0.37,gene diversity within populations(HS)was 0.28,gene diversity among populations(HST)was 0.09 and the coefficient of gene differentiation(GST)among the populations was 0.23,indicating that 76.7% of variation was within populations while 23.3% was among populations,which showed that there was rich genetic variation within population variation,just the same as Shannon's diversity index.Gene flow(Nm)from inter-population was 0.74,which indicated the limited genetic exchange between populations.