C. Xiaodan, Zhu Liquan, W. Yong, Rong Xiaoying, Lu Jun, Wang Xiao-jia
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引用次数: 0
Abstract
In exploring an effective and reliable karyotyping method in Brassica crop plants, Brassica oleracea was successfully karyotyped using genomic in situ hybridization (GISH). B. oleracea genomic DNA was labelled as probe using DIG-high prime mix kit, with B. rapa genomic DNA acting as blocking agent. Specific fluorescent signals were detected on each pair of homologous chromosomes, and nine pairs of chromosomes of B. oleracea were clearly identified according to the signal characteristics. A practical and accurate method for conducting karyotyping of small chromosomes has been demonstrated.
为探索一种有效、可靠的芸苔属作物核型方法，利用基因组原位杂交(GISH)技术成功地对芸苔属植物进行了核型分析。采用digi -high prime mix kit标记甘蓝基因组DNA作为探针，以甘蓝基因组DNA作为阻断剂。在每对同源染色体上检测特异性荧光信号，根据信号特征明确鉴定出甘蓝的9对染色体。已经证明了一种实用而准确的方法来进行小染色体的核型。
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