Novel Lactobacillus Fermentation Prediction Using Deep Learning

Jain-Shing Wu, Chien-Chang Wu, Chien-Sen Liao
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引用次数: 0

Abstract

In recent years, due to the vigorous development of artificial intelligence in various fields, many various applications have appeared. However, due to the biological uncertainty, only a few research apply artificial intelligence to manage the biological production process. The fermentation process of lactic acid bacteria has biologically uncertain, and the parameters in the fermentation process are difficult to set with fixed values to be automatically executed. Therefore, the current fermentation process is carried out manually. Due to the uncertainty in the production process, once human error occurs, it often causes hundreds of thousands or even millions dollars of losses. Therefore, if the fermentation effect can be improved, the subsequent production efficiency can be directly improved. In order to automate the fermentation process, in this project, we hope that by combining artificial intelligence (AI) with the background of lactic acid bacteria cultivation, the current complicated manual fermentation process can be transformed into automation as the goal of Industry 4.0. Based on the logs of the experiments of Lactobacillus fermentation, we use Long Shorten-Memory (LSTM) to predict the output amount of fermentation results. In the experimental results, we collects 9 trials of experimental results (4 case for over 3*109, 5 cases for approaching 3*109 and 7 cases for 0 output). And then, all the results are randomly separated into training and testing datasets for 20 different runs. The training dataset average accuracy of 20 runs is 100%. And the testing dataset average accuracy of 20 runs is 95%. Hence, according to the experimental results, we can know the proposed methods really can predicted the amount of the fermentation products.
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基于深度学习的新型乳酸杆菌发酵预测
近年来,由于人工智能在各个领域的蓬勃发展,出现了许多不同的应用。然而,由于生物的不确定性,应用人工智能管理生物生产过程的研究很少。乳酸菌发酵过程具有生物学上的不确定性,发酵过程中的参数难以设定固定值并自动执行。因此,目前的发酵过程是手工进行的。由于生产过程中的不确定性,一旦发生人为失误,往往会造成数十万甚至数百万美元的损失。因此,如果能提高发酵效果,就能直接提高后续的生产效率。为了实现发酵过程的自动化,在这个项目中,我们希望通过将人工智能(AI)与乳酸菌培养的背景相结合,将目前复杂的人工发酵过程转化为自动化,作为工业4.0的目标。根据乳酸菌发酵实验日志,采用长短时记忆法(LSTM)预测发酵结果的输出量。在实验结果中,我们收集了9例实验结果(超过3*109 4例,接近3*109 5例,0输出7例)。然后,所有的结果被随机分为20个不同运行的训练和测试数据集。20次运行的训练数据集平均准确率为100%。测试数据集20次运行的平均准确率为95%。因此,根据实验结果,我们可以知道所提出的方法确实可以预测发酵产物的数量。
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